Real time monitoring and quantification of reactive oxygen species in breast cancer cell line MCF-7 by 2′,7′–dichlorofluorescin diacetate (DCFDA) assay

Daniela Figueroa1, Mohammad Asaduzzaman1, Fiona Young1
1Department of Medical Biotechnology, College of Medicine and Public Health, Flinders University, Adelaide, SA 5052, Australia

Tài liệu tham khảo

Alia, 2005, Response of the antioxidant defense system to tert-butyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2), Journal of Biochemical and Molecular Toxicology, 19, 119, 10.1002/jbt.20061 Andoh, 2006, The antioxidant role of a reagent, 2′, 7′-dichlorodihydrofluorescin diacetate, detecting reactive-oxygen species and blocking the induction of Heme Oxygenase-1 and preventing cytotoxicity, Journal of Biochemistry, 140, 10.1093/jb/mvj187 Bass, 1983, Flow cytometric studies of oxidative product formation by neutrophils: A graded response to membrane stimulation, The Journal of Immunology, 130, 1910, 10.4049/jimmunol.130.4.1910 Bechtel, 2001, Inhibitory effects of 17β-estradiol and progesterone on ovarian carcinoma cell proliferation: A potential role for inducible nitric oxide synthase, Gynecologic Oncology, 82, 127, 10.1006/gyno.2001.6221 Benhar, 2002, ROS, stress-activated kinases and stress signaling in cancer, EMBO Reports, 3, 420, 10.1093/embo-reports/kvf094 Brandt, 1965, Synthesis of diacetyldichlorofluorescin: A stable reagent for fluorometric analysis, Analytical Biochemistry, 11, 6, 10.1016/0003-2697(65)90035-7 Brubacher, 2001, Chemically de-acetylated 2′,7′-dichlorodihydrofluorescein diacetate as a probe of respiratory burst activity in mononuclear phagocytes, Journal of Immunological Methods, 251, 81, 10.1016/S0022-1759(01)00308-8 Cadenas, 2004, Mitochondrial free radical production and cell signaling, Molecular Aspects of Medicine, 25, 17, 10.1016/j.mam.2004.02.005 Chen, 2010, 2′, 7′-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy, Free Radical Research, 44, 587, 10.3109/10715761003709802 Cooke, 2003, Oxidative DNA damage: Mechanisms, mutation, and disease, The FASEB Journal, 17, 1195, 10.1096/fj.02-0752rev Dröge, 2002, Free radicals in the physiological control of cell function, Physiological Reviews, 82, 47, 10.1152/physrev.00018.2001 Epling, 1992, Neutrophil function screenlng in patients with chronic granulomatous disease by a flow cytometric method, Cytometry, 13, 615, 10.1002/cyto.990130609 Fan, 2014, Evaluation of methods of detecting cell reactive oxygen species production for drug screening and cell cycle studies, Journal of Pharmacological and Toxicological Methods, 70, 40, 10.1016/j.vascn.2014.03.173 Fink, 2002, Role of reactive oxygen and nitrogen species in acute respiratory distress syndrome, Current Opinion in Critical Care, 8, 6, 10.1097/00075198-200202000-00002 Finkel, 2011, Signal transduction by reactive oxygen species, The Journal of Cell Biology, 194, 7, 10.1083/jcb.201102095 Forkink, 2010, Detection and manipulation of mitochondrial reactive oxygen species in mammalian cells, Biochimica et Biophysica acta (BBa)-Bioenergetics, 1797, 1034, 10.1016/j.bbabio.2010.01.022 Giorgio, 2007, Hydrogen peroxide: A metabolic by-product or a common mediator of ageing signals?, Nature Reviews Molecular Cell Biology, 8, 722, 10.1038/nrm2240 Gomes, 2005, Fluorescence probes used for detection of reactive oxygen species, Journal of Biochemical and Biophysical Methods, 65, 45, 10.1016/j.jbbm.2005.10.003 Gong, 2016, Phosphorus, and nitrogen co-doped carbon dots as a fluorescent probe for real-time measurement of reactive oxygen and nitrogen species inside macrophages, Biosensors and Bioelectronics, 79, 822, 10.1016/j.bios.2016.01.022 Guidarelli, 1997, Alternative mechanisms for hydroperoxide-induced DNA single strand breakage, Free Radical Research, 26, 537, 10.3109/10715769709097825 Gutteridge, 2010, Antioxidants: Molecules, medicines, and myths, Biochemical and Biophysical Research Communications, 393, 561, 10.1016/j.bbrc.2010.02.071 Halliwell, 1991, Drug antioxidant effects, Drugs, 42, 569, 10.2165/00003495-199142040-00003 Halliwell, 2004, Measuring reactive species and oxidative damage in vivo and in cell culture: How should you do it and what do the results mean?, British Journal of Pharmacology, 142, 231, 10.1038/sj.bjp.0705776 Hawkes, 2010, Regulation of redox signaling by selenoproteins, Biological Trace Element Research, 134, 235, 10.1007/s12011-010-8656-7 Hoshi, 2001, Regulation of cell function by methionine oxidation and reduction, The Journal of Physiology, 531, 1, 10.1111/j.1469-7793.2001.0001j.x Jackson, 2001, The contribution of endogenous sources of DNA damage to the multiple mutations in cancer, Mutation Research, Fundamental and Molecular Mechanisms of Mutagenesis, 477, 7, 10.1016/S0027-5107(01)00091-4 Kim, 1998, Role of Ca^ 2^+ influx in the tert-butyl hydroperoxide-induced apoptosis of HepG2 human hepatoblastoma cells, Experimental and Molecular Medicine, 30, 137, 10.1038/emm.1998.20 Kim, 2005, The isolation and antioxidative effects of vitexin fromAcer palmatum, Archives of Pharmacal Research, 28, 195, 10.1007/BF02977715 Kučera, 2014, The effect of tert-butyl hydroperoxide-induced oxidative stress on lean and steatotic rat hepatocytes in vitro, Oxidative Medicine and Cellular Longevity, 2014, 10.1155/2014/752506 Kweon, 2004, Cytoprotective effects of heme oxygenase-1 induction by 3-O-caffeoyl-1-methylquinic acid, Free Radical Biology and Medicine, 36, 40, 10.1016/j.freeradbiomed.2003.09.023 Lautraite, 2003, Optimisation of cell-based assays for medium throughput screening of oxidative stress, Toxicology In Vitro, 17, 207, 10.1016/S0887-2333(03)00005-5 Lee, 2006, The PerR transcription factor senses H2O2 by metal-catalysed histidine oxidation, Nature, 440, 363, 10.1038/nature04537 Li, 2004, Endothelial cell superoxide generation: Regulation and relevance for cardiovascular pathophysiology, American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 287, R1014, 10.1152/ajpregu.00124.2004 Liou, 2010, Reactive oxygen species in cancer, Free Radical Research, 44, 479, 10.3109/10715761003667554 Maneesh, 2006, Role of reactive oxygen species and antioxidants on pathophysiology of male reproduction, Indian Journal of Clinical Biochemistry, 21, 80, 10.1007/BF02912918 Martin, 2001, Tert-butyl hydroperoxide-induced lipid signaling in hepatocytes: Involvement of glutathione and free radicals, Biochemical Pharmacology, 62, 705 Mehanna, 2011, Spectrofluorometry assays for oxidative stress and apoptosis, with cell viability on the same microplates: A multiparametric analysis and quality control, Toxicology In Vitro, 25, 1089, 10.1016/j.tiv.2011.03.007 Menon, 2007, A redox cycle within the cell cycle: Ring in the old with the new, Oncogene, 26, 1101, 10.1038/sj.onc.1209895 Miki, 2012, Regulation of intracellular signalling through cysteine oxidation by reactive oxygen species, Journal of Biochemistry, 151, 255, 10.1093/jb/mvs006 Murrant, 2001, Detection of reactive oxygen and reactive nitrogen species in skeletal muscle, Microscopy Research and Technique, 55, 236, 10.1002/jemt.1173 Oparka, 2016, Quantifying ROS levels using CM-H2DCFDA and HyPer, Methods, 109, 3, 10.1016/j.ymeth.2016.06.008 Reid, 2015, Undifferentiated murine embryonic stem cells used to model the effects of the blue–green algal toxin cylindrospermopsin on preimplantation embryonic cell proliferation, Toxicon, 106, 79, 10.1016/j.toxicon.2015.09.022 Rosenkranz, 1992, A microplate assay for the detection of oxidative products using 2′, 7′-dichlorofluorescin-diacetate, Journal of Immunological Methods, 156, 39, 10.1016/0022-1759(92)90008-H Rota, 1999, Evidence for free radical formation during the oxidation of 2′-7′-dichlorofluorescin to the fluorescent dye 2′-7′-dichlorofluorescein by horseradish peroxidase: Possible implications for oxidative stress measurements, Free Radical Biology and Medicine, 27, 873, 10.1016/S0891-5849(99)00137-9 Rota, 1999, Phenoxyl free radical formation during the oxidation of the fluorescent dye 2′, 7′-Dichlorofluorescein by horseradish peroxidase possible consequences for oxidative stress measurements, Journal of Biological Chemistry, 274, 28161, 10.1074/jbc.274.40.28161 Royall, 1993, Evaluation of 2′, 7′-dichlorofluorescin and dihydrorhodamine 123 as fluorescent probes for intracellular H2O2 in cultured endothelial cells, Archives of Biochemistry and Biophysics, 302, 348, 10.1006/abbi.1993.1222 Sandström, 1991, Induction and rejoining of DNA single-strand breaks in relation to cellular growth in human cells exposed to three hydroperoxides at 0 C and 37 C, Free Radical Research Communications, 15, 79, 10.3109/10715769109049128 Saotome, 1989, Cytotoxicity test with simplified crystal violet staining method using microtitre plates and its application to injection drugs, Toxicology In Vitro, 3, 317, 10.1016/0887-2333(89)90039-8 Siddiqui, 2006, The effect of serotonin and serotonin antagonists on bladder cancer cell proliferation, BJU International, 97, 634, 10.1111/j.1464-410X.2006.06056.x Simon, 2000, Role of reactive oxygen species (ROS) in apoptosis induction, Apoptosis, 5, 415, 10.1023/A:1009616228304 Storz, 2005, Reactive oxygen species in tumor progression, Frontiers in Bioscience, 10, 1881, 10.2741/1667 Tarpey, 2001, Methods of detection of vascular reactive species, Circulation Research, 89, 224, 10.1161/hh1501.094365 Valko, 2007, Free radicals and antioxidants in normal physiological functions and human disease, The International Journal of Biochemistry & Cell Biology, 39, 44, 10.1016/j.biocel.2006.07.001 Verbon, 2012, The influence of reactive oxygen species on cell cycle progression in mammalian cells, Gene, 511, 1, 10.1016/j.gene.2012.08.038 Vurusaner, 2012, Tumor suppressor genes and ROS: Complex networks of interactions, Free Radical Biology and Medicine, 52, 7, 10.1016/j.freeradbiomed.2011.09.035 Wan, 2003, Adaptation of the dichlorofluorescein assay for detection of radiation-induced oxidative stress in cultured cells, Radiation Research, 160, 622, 10.1667/3099 Wan, 2005, Standardization of a fluorometric assay for measuring oxidative stress in irradiated cells, Radiation Research, 163, 232, 10.1667/RR3299 Winterbourn, 2014, The challenges of using fluorescent probes to detect and quantify specific reactive oxygen species in living cells, Biochimica et Biophysica Acta (BBA) - General Subjects, 1840, 730, 10.1016/j.bbagen.2013.05.004 Woolley, 2013, Recent advances in reactive oxygen species measurement in biological systems, Trends in Biochemical Sciences, 38, 556, 10.1016/j.tibs.2013.08.009 Wrona, 2005, Reactivity of 2′, 7′-dichlorodihydrofluorescein and dihydrorhodamine 123 and their oxidized forms toward carbonate, nitrogen dioxide, and hydroxyl radicals, Free Radical Biology and Medicine, 38, 262, 10.1016/j.freeradbiomed.2004.10.022 Zhou, 1997, A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: Applications in detecting the activity of phagocyte NADPH oxidase and other oxidases, Analytical Biochemistry, 253, 162, 10.1006/abio.1997.2391