Đo độ huỳnh quang tỷ lệ cho troponin-I tim sử dụng chấm carbon và nanocube palladium-iridium có hoạt tính mô phỏng peroxidase

Microchimica Acta - Tập 186 - Trang 1-8 - 2019
Xiaofeng Tan1, Lianhua Zhang2, Qiaorong Tang1, Gengxiu Zheng1, He Li1,3
1School of Chemistry and Chemical Engineering, and Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan, China
2Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
3College of Optoelectronics Technology, Chengdu University of Information Technology, Chengdu, China

Tóm tắt

Một phương pháp xét nghiệm miễn dịch liên kết nanozyme được mô tả cho troponin I tim, biomarker cho nhồi máu cơ tim. Phương pháp này dựa trên việc sử dụng các nanocube Pd-Ir với hoạt tính giống như peroxidase xuất sắc. Các nanocube này xúc tác quá trình oxi hóa o-phenylenediamine (OPD) không huỳnh quang bằng H2O2 để tạo thành một sản phẩm huỳnh quang màu vàng (oxOPD) với các đỉnh hấp thụ/phát xạ lần lượt tại 400/570 nm. Các chấm carbon được thêm vào như một fluorophore tham chiếu. Dưới cùng một bước sóng kích thích, chúng phát huỳnh quang màu xanh (450 nm). ELISA sử dụng các nanocube Pd-Ir làm nhãn cho kháng thể thứ cấp và OPD làm chất nền. Tỷ lệ cường độ huỳnh quang tại 570 và 450 nm tăng lên trong khoảng nồng độ troponin I tim từ 1 pg·mL−1 đến 1 ng·mL−1, và giới hạn phát hiện là 0.31 pg·mL−1. Phương pháp đã được áp dụng để phân tích các mẫu huyết thanh đã được tăng cường, và kết quả thu được có sự tương đồng tốt với những kết quả đạt được từ một xét nghiệm hóa phát quang thương mại.

Từ khóa

#troponin I #nhồi máu cơ tim #xét nghiệm miễn dịch #nanocube #palladium #iridium #peroxidase #huỳnh quang

Tài liệu tham khảo

Han S, Zhou T, Yin B, He P (2018) Gold nanoparticle-based colorimetric ELISA for quantification of ractopamine. Microchim Acta 185(4):210 Zeng K, Tian S, Wang Z, Shen C, Luo J, Yang M, Liu Y-N (2017) An ELISA for the determination of human IgG based on the formation of a colored iron (II) complex and photometric or visual read-out. Microchim Acta 184(8):2791–2796 Nasir M, Nawaz MH, Latif U, Yaqub M, Hayat A, Rahim A (2016) An overview on enzyme-mimicking nanomaterials for use in electrochemical and optical assays. Microchim Acta 184(2):1–20 Sun J, Ge J, Liu W, Lan M, Zhang H, Wang P, Wang Y, Niu Z (2014) Multi-enzyme co-embedded organic–inorganic hybrid nanoflowers: synthesis and application as a colorimetric sensor. Nanoscale 6(1):255–262 Huang L, Zhang W, Chen K, Zhu W, Liu X, Wang R, Zhang X, Hu N, Suo Y, Wang J (2017) Facet-selective response of trigger molecule to CeO2 {1 1 0} for up-regulating oxidase-like activity. Chem Eng J 330:746–752 Ye R, Zhu C, Song Y, Lu Q, Ge X, Yang X, Zhu MJ, Du D, Li H, Lin Y (2016) Bioinspired synthesis of all-in-one organic–inorganic hybrid Nanoflowers combined with a handheld pH meter for on-site detection of food pathogen. Small 12(23):3094–3100 Ye R, Zhu C, Song Y, Song J, Fu S, Lu Q, Yang X, Zhu M-J, Du D, Li H (2016) One-pot bioinspired synthesis of all-inclusive protein–protein nanoflowers for point-of-care bioassay: detection of E. Coli O157: H7 from milk. Nanoscale 8(45):18980–18986 Hou C, Wang Y, Ding Q, Jiang L, Li M, Zhu W, Pan D, Zhu H, Liu M (2015) Facile synthesis of enzyme-embedded magnetic metal–organic frameworks as a reusable mimic multi-enzyme system: mimetic peroxidase properties and colorimetric sensor. Nanoscale 7(44):18770–18779 Cheng Y-J, Luo G-F, Zhu J-Y, Xu X-D, Zeng X, Cheng D-B, Li Y-M, Wu Y, Zhang X-Z, Zhuo R-X (2015) Enzyme-induced and tumor-targeted drug delivery system based on multifunctional mesoporous silica nanoparticles. ACS Appl Mater Interfaces 7(17):9078–9087 Farka ZK, Čunderlová V, HoráČková V, Pastucha MJ, Mikušová Z, HlaváČek AN, Skládal P (2018) Prussian blue nanoparticles as a catalytic label in a Sandwich Nanozyme-linked immunosorbent assay. Anal Chem 90(3):2348–2354 Xia X, Zhang J, Lu N, Kim MJ, Ghale K, Xu Y, McKenzie E, Liu J, Ye H (2015) Pd–Ir core–shell nanocubes: a type of highly efficient and versatile peroxidase mimic. ACS Nano 9(10):9994–10004 Wu Q, Li S, Sun Y, Wang J (2017) Hollow gold nanoparticle-enhanced SPR based sandwich immunoassay for human cardiac troponin I. Microchim Acta 184(7):2395–2402 Tao Y, Ju E, Ren J, Qu X (2015) Bifunctionalized mesoporous silica-supported gold nanoparticles: intrinsic oxidase and peroxidase catalytic activities for antibacterial applications. Adv Mater 27(6):1097–1104 Lan J, Xu W, Wan Q, Zhang X, Lin J, Chen J, Chen J (2014) Colorimetric determination of sarcosine in urine samples of prostatic carcinoma by mimic enzyme palladium nanoparticles. Anal Chim Acta 825:63–68 Chen M, Shu J, Wang Z, Ren C (2017) Porous surface MnO2 microspheres as oxidase mimetics for colorimetric detection of sulfite. J Porous Mater 24(4):973–977 He W, Liu Y, Yuan J, Yin J-J, Wu X, Hu X, Zhang K, Liu J, Chen C, Ji Y (2011) Au@ Pt nanostructures as oxidase and peroxidase mimetics for use in immunoassays. Biomaterials 32(4):1139–1147 Gao Z, Ye H, Tang D, Tao J, Habibi S, Minerick A, Tang D, Xia X (2017) Platinum-decorated gold nanoparticles with dual functionalities for ultrasensitive colorimetric in vitro diagnostics. Nano Lett 17(9):5572–5579 Benuzzi MLS, Pereira SV, Raba J, Messina GA (2016) Screening for cystic fibrosis via a magnetic and microfluidic immunoassay format with electrochemical detection using a copper nanoparticle-modified gold electrode. Microchim Acta 183(1):397–405 Liang Y, Huang X, Yu R, Zhou Y, Xiong Y (2016) Fluorescence ELISA for sensitive detection of ochratoxin a based on glucose oxidase-mediated fluorescence quenching of CdTe QDs. Anal Chim Acta 936:195–201 Tao L, Zhang C, Zhang J, Sun Y, Li X, Yan K, Jin B, Zhang Z, Yang K (2016) Sensitive chemiluminescence immunoassay for staphylococcal enterotoxin C1 based on the use of dye-encapsulated mesoporous silica nanoparticles. Microchim Acta 183(7):2163–2168 Zhang W-H, Ma W, Long Y-T (2016) Redox-mediated indirect fluorescence immunoassay for the detection of disease biomarkers using dopamine-functionalized quantum dots. Anal Chem 88(10):5131–5136 Guo X, Wu F, Ni Y, Kokot S (2016) Synthesizing a nano-composite of BSA-capped au nanoclusters/graphitic carbon nitride nanosheets as a new fluorescent probe for dopamine detection. Anal Chim Acta 942:112–120 Wu Y, Wei P, Pengpumkiat S, Schumacher EA, Remcho VT (2015) Development of a carbon dot (C-dot)-linked immunosorbent assay for the detection of human α-fetoprotein. Anal Chem 87(16):8510–8516 Beloglazova N, Speranskaya E, Wu A, Wang Z, Sanders M, Goftman V, Zhang D, Goryacheva IY, De Saeger S (2014) Novel multiplex fluorescent immunoassays based on quantum dot nanolabels for mycotoxins determination. Biosens Bioelectron 62:59–65 Wang L, Xu M, Huang R, Chang X, Chen C, Li L, Zhang Z, Han Y (2017) A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of cardiac troponin T and myoglobin. Slas Technol 22(2):130–135 Cheng H, Lin S, Muhammad F, Lin Y-W, Wei H (2016) Rationally modulate the oxidase-like activity of nanoceria for self-regulated bioassays. ACS Sensors 1(11):1336–1343 Seo S-M, Kim S-W, Park J-N, Cho J-H, Kim H-S, Paek S-H (2016) A fluorescent immunosensor for high-sensitivity cardiac troponin I using a spatially-controlled polymeric, nano-scale tracer to prevent quenching. Biosens Bioelectron 83:19–26 Lee MH, Kim JS, Sessler JL (2015) Small molecule-based ratiometric fluorescence probes for cations, anions, and biomolecules. Chem Soc Rev 44(13):4185–4191 Ma F, Sun M, Zhang K, Wang S (2015) A ratiometric fluorescence sensor for highly selective and sensitive detection of mercuric ion. Sensors Actuators B Chem 209:377–383 Zhu S, Meng Q, Wang L, Zhang J, Song Y, Jin H, Zhang K, Sun H, Wang H, Yang B (2013) Highly photoluminescent carbon dots for multicolor patterning, sensors, and bioimaging. Angew Chem 125(14):4045–4049 Wang N, Duan J, Shi W, Zhai X, Guan F, Yang L, Hou B (2018) A 3-dimensional C/CeO 2 hollow nanostructure framework as a peroxidase mimetic, and its application to the colorimetric determination of hydrogen peroxide. Microchim Acta 185(9):417 Zarif F, Rauf S, Qureshi MZ, Shah NS, Hayat A, Muhammad N, Rahim A, Nawaz MH, Nasir M (2018) Ionic liquid coated iron nanoparticles are promising peroxidase mimics for optical determination of H 2 O 2. Microchim Acta 185(6):302 Dehghani Z, Hosseini M, Mohammadnejad J, Bakhshi B, Rezayan AH (2018) Colorimetric aptasensor for campylobacter jejuni cells by exploiting the peroxidase like activity of au@ Pd nanoparticles. Microchim Acta 185(10):448 Peng Y, Shen H, Tang S, Huang Z, Hao Y, Luo Z, Zhou F, Wang T, Feng W (2018) Colorimetric determination of BCR/ABL fusion genes using a nanocomposite consisting of au@ Pt nanoparticles covered with a PAMAM dendrimer and acting as a peroxidase mimic. Microchim Acta 185(8):401 Khataee A, Irani-Nezhad MH, Hassanzadeh J (2018) Improved peroxidase mimetic activity of a mixture of WS 2 nanosheets and silver nanoclusters for chemiluminescent quantification of H 2 O 2 and glucose. Microchim Acta 185(3):190 Nirala NR, Prakash R (2018) Quick colorimetric determination of choline in milk and serum based on the use of MoS 2 nanosheets as a highly active enzyme mimetic. Microchim Acta 185(4):224 Wang G-L, Hu X-L, Wu X-M, Li Z-J (2014) Quantum dots-based glucose sensing through fluorescence quenching by bienzyme-catalyzed chromogenic substrate oxidation. Sensors Actuators B Chem 205:61–66 Zhang F, Liu H, Liu Q, Su X (2018) An enzymatic ratiometric fluorescence assay for 6-mercaptopurine by using MoS 2 quantum dots. Microchim Acta 185(12):540 Lakowicz JR (2006) Principles of fluorescence spectroscopy, 3rd edn. Springer, Baltimore Zong C, Zhang D, Yang H, Wang S, Chu M, Li P (2017) Chemiluminescence immunoassay for cardiac troponin T by using silver nanoparticles functionalized with hemin/G-quadruplex DNAzyme on a glass chip array. Microchim Acta 184(9):3197–3204 Harris D C (1995) Quantitative chemical analysis, 4th edn. In: Freeman W H (ed) Company Press, pp 123–153