Rat pro-inflammatory cytokine and cytokine related mRNA quantification by real-time polymerase chain reaction using SYBR green

Giulietti A, Overbergh L, Valckx D, Decallonne B, Bouillon R, Mathieu C: An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods. 2001, 25: 386-401. 10.1006/meth.2001.1261.

Melchior B, Remy S, Nerriere-Daguin V, Heslan JM, Soulillou JP, Brachet P: Temporal analysis of cytokine gene expression during infiltration of porcine neuronal grafts implanted into the rat brain. J Neurosci Res. 2002, 68: 284-292. 10.1002/jnr.10216.

Walch L, Morris PL: Cyclooxygenase 2 pathway mediates IL-1beta regulation of IL-1alpha, -1beta, and IL-6 mRNA levels in Leydig cell progenitors. Endocrinology. 2002, 143: 3276-3283. 10.1210/en.2002-220410.

Li X, Wang X: Application of real-time polymerase chain reaction for the quantitation of interleukin-1beta mRNA upregulation in brain ischemic tolerance. Brain Res Brain Res Protoc. 2000, 5: 211-217. 10.1016/S1385-299X(00)00015-5.

Krook H, Wennberg L, Hagberg A, Song Z, Groth CG, Korsgren O: Immunosuppressive drugs in islet xenotransplantation: a tool for gaining further insights in the mechanisms of the rejection process. Transplantation. 2002, 74: 1084-1089. 10.1097/00007890-200210270-00005.

Harness J, Pender MP, McCombe PA: Cyclosporin A treatment modulates cytokine mRNA expression by inflammatory cells extracted from the spinal cord of rats with experimental autoimmune encephalomyelitis induced by inoculation with myelin basic protein. J Neurol Sci. 2001, 187: 7-16. 10.1016/S0022-510X(01)00505-6.

Muhallab S, Lundberg C, Gielen AW, Lidman O, Svenningsson A, Piehl F, Olsson T: Differential expression of neurotrophic factors and inflammatory cytokines by myelin basic protein-specific and other recruited T cells infiltrating the central nervous system during experimental autoimmune encephalomyelitis. Scand J Immunol. 2002, 55: 264-273. 10.1046/j.0300-9475.2002.01038.x.

Stordeur P, Poulin LF, Craciun L, Zhou L, Schandene L, de Lavareille A, Goriely S, Goldman M: Cytokine mRNA quantification by real-time PCR. J Immunol Methods. 2002, 259: 55-64. 10.1016/S0022-1759(01)00489-6.

Overbergh L, Valckx D, Waer M, Mathieu C: Quantification of murine cytokine mRNAs using real-time quantitative reverse transcriptase PCR. Cytokine. 1999, 11: 305-312. 10.1006/cyto.1998.0426.

Yin JL, Shackel NA, Zekry A, McGuinness PH, Richards C, Putten KV, McCaughan GW, Eris JM, Bishop GA: Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR Green I. Immunol Cell Biol. 2001, 79: 213-221. 10.1046/j.1440-1711.2001.01002.x.

Lekanne Deprez RH, Fijnvandraat AC, Ruijter JM, Moorman AF: Sensitivity and accuracy of quantitative real-time polymerase chain reaction using SYBR green I depends on cDNA synthesis conditions. Anal Biochem. 2002, 307: 63-69. 10.1016/S0003-2697(02)00021-0.

Lestaevel P, Agay D, Peinnequin A, Cruz C, Cespuglio R, Clarencon D, Multon E, Chancerelle Y: Effects of a thermal injury on brain and blood nitric oxide (NO) content in the rat. Burns. 2003, 29: 557-562. 10.1016/S0305-4179(03)00152-9.

Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001, 25: 402-408. 10.1006/meth.2001.1262.

Leutenegger CM, Mislin CN, Sigrist B, Ehrengruber MU, Hofmann-Lehmann R, Lutz H: Quantitative real-time PCR for the measurement of feline cytokine mRNA. Vet Immunol Immunopathol. 1999, 71: 291-305. 10.1016/S0165-2427(99)00100-2.

Schmittgen TD, Zakrajsek BA, Mills AG, Gorn V, Singer MJ, Reed MW: Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Anal Biochem. 2000, 285: 194-204. 10.1006/abio.2000.4753.

Teo IA, Choi JW, Morlese J, Taylor G, Shaunak S: LightCycler qPCR optimisation for low copy number target DNA. J Immunol Methods. 2002, 270: 119-133. 10.1016/S0022-1759(02)00218-1.

Birot OJ, Koulmann N, Peinnequin A, Bigard XA: Exercise-induced expression of vascular endothelial growth factor mRNA in rat skeletal muscle is dependent on fibre type. J Physiol. 2003, 552: 213-221. 10.1113/jphysiol.2003.043026.

Norol F, Merlet P, Isnard R, Sebilion P, Bonnet N, Cailliot C, Carrion C, Ribeiro M, Charlotte F, Pradeau P, Mayol JF, Peinnequin A, Drouet M, Safsafi K, Vernant JP, Herodin F: Influence of mobilized stem cells on myocardial infarct repair in a nonhuman primate model. Blood. 2003, 102: 4361-4368. 10.1182/blood-2003-03-0685.

Gilliland G, Perrin S, Blanchard K, Bunn HF: Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci U S A. 1990, 87: 2725-2729.

Gisselbrecht S: The CIS/SOCS proteins: a family of cytokine-inducible regulators of signaling. Eur Cytokine Netw. 1999, 10: 463-470.

Lang R, Pauleau AL, Parganas E, Takahashi Y, Mages J, Ihle JN, Rutschman R, Murray PJ: SOCS3 regulates the plasticity of gp130 signaling. Nat Immunol. 2003, 4: 546-550. 10.1038/ni932.

Lestaevel P, Clarençon D, Gharib A, Peinnequin A, Cespuglio R, Gourmelon P, Alonso A, J.D. Laval, Multon E: Nitric oxide voltammetric measurements in the rat brain after Gamma irradiation. Radiation Research. 2003, 160: 631-636.

Igwe OJ, Murray JN, Moolwaney AS: Interleukin 1-induced cyclooxygenase and nitric oxide synthase gene expression in the rat dorsal root ganglia is modulated by antioxidants. Neuroscience. 2001, 105: 971-985. 10.1016/S0306-4522(01)00253-6.

Akira S, Kishimoto T: IL-6 and NF-IL6 in acute-phase response and viral infection. Immunol Rev. 1992, 127: 25-50.

Yang Y, Pares-Matos EI, Tesmer VM, Dai C, Ashworth S, Huai J, Bina M: Organization of the promoter region of the human NF-IL6 gene. Biochim Biophys Acta. 2002, 1577: 102-108. 10.1016/S0167-4781(02)00401-3.

Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987, 162: 156-159. 10.1006/abio.1987.9999.