Rapid nanopore discrimination between single polynucleotide molecules

Proceedings of the National Academy of Sciences of the United States of America - Tập 97 Số 3 - Trang 1079-1084 - 2000
A. Meller1, Lucas G. Nivón1, Eric Brandin1, J. A. Golovchenko1, Daniel Branton1
1Departments of Molecular and Cellular Biology and Physics, Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138; Center for Advanced Biotechnology, Biomedical Engineering, Boston University, Boston, MA 02215; and The Rowland Institute for Science, Cambridge, MA 02142-1297

Tóm tắt

A variety of different DNA polymers were electrophoretically driven through the nanopore of an α-hemolysin channel in a lipid bilayer. Single-channel recording of the translocation duration and current flow during traversal of individual polynucleotides yielded a unique pattern of events for each of the several polymers tested. Statistical data derived from this pattern of events demonstrate that in several cases a nanopore can distinguish between polynucleotides of similar length and composition that differ only in sequence. Studies of temperature effects on the translocation process show that translocation duration scales as ∼ T −2 . A strong correlation exists between the temperature dependence of the event characteristics and the tendency of some polymers to form secondary structure. Because nanopores can rapidly discriminate and characterize unlabeled DNA molecules at low copy number, refinements of the experimental approach demonstrated here could eventually provide a low-cost high-throughput method of analyzing DNA polynucleotides.

Từ khóa


Tài liệu tham khảo

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Thông tin xuất bản

Proceedings of the National Academy of Sciences of the United States of America

Tập 97 Số 3

1079-1084

Thông tin tác giả