Rapid method for identification of chemopreventive compounds using multiplex RT-PCR for cyclooxygenase mRNA expression

Prescott, 2000, Cyclooxygenase-2 and carcinogenesis, Biochim. Biophys. Acta, 1470, M69 Steele, 2003, Mechanisms and applications of the non-steroidal anti-inflammatory drugs in the chemoprevention of cancer, Mutat. Res., 523/524, 137, 10.1016/S0027-5107(02)00329-9 Crofford, 1997, COX-1 and COX-2 tissue expression: implications and predictions, J. Rheumatol., 24, 5 Yang, 2001, Inhibition of carcinogenesis by dietary polyphenolic compounds, Annu. Rev. Nutr., 21, 381, 10.1146/annurev.nutr.21.1.381 Surh, 2001, Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals downregulation of COX-2 and iNOS through suppression of NF-κBV activation, Mutat. Res., 480/481, 243, 10.1016/S0027-5107(01)00183-X Surh, 2002, Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review, Food Chem. Toxicol., 20, 1091, 10.1016/S0278-6915(02)00037-6 Malik, 2003, Anthocyanin-rich extract from Aronia meloncarpa E. induces a cell cycle block in colon cancer but not normal colonic cells, Nutr. Cancer, 46, 186, 10.1207/S15327914NC4602_12 Dommels, 2003, The role of cyclooxygenase in n-6 and n-3 polyunsaturated fatty acid mediated effects on cell proliferation, PGE(2) synthesis and cytotoxicity in human colorectal carcinoma cell lines, Carcinogenesis, 24, 385, 10.1093/carcin/24.3.385 Grosch, 2001, COX-2 independent induction of cell cycle arrest and apoptosis in colon cancer cells by the selective COX-2 inhibitor celecoxib, FASEB J., 15, 2742, 10.1096/fj.01-0299fje