Rapid marker-assisted development of advanced recombinant lines from barley starch mutants
Tóm tắt
The comparative phenotypic analysis of mutants is often hampered by their diverse and poorly characterised genetic backgrounds. To overcome this problem, a suite of recombinant spring barley lines was developed for four starch biosynthesis genes in a common elite background. Rapid breeding progress was made by combining foreground and background selection with the screening of bulked families. A toolkit of perfect co-dominant PCR assays was developed for the four target genes, based on the causative single nucleotide polymorphisms underlying their starch phenotypes. These were used for foreground selection during backcrossing and selfing, and may be applied to bulks of up to ten plants. Screening bulks meant that large numbers of individuals with known family structure were rapidly assessed and that breeding effort was accurately targeted. These markers were also used for quality control during field multiplication and should be readily transferable to any crosses involving these four mutations. Background selection amongst BC1 progeny known to be heterozygous for the target starch alleles identified individuals which were relatively enriched for the recurrent parent across the rest of the genome. These were further advanced and true-breeding recombinants were selected which carry the target starch mutations in a largely recurrent parent background. The resulting set of BC2F5 pre-breeding lines should enable meaningful analysis of the starch phenotypes and facilitate their transfer into commercial breeding programmes.
Tài liệu tham khảo
Biyashev RM, Netsvetaev VP, Sozinov AA (1986) Genetic control of some morphological markers for qualitative and biochemical characters and location of three genetic factors on chromosomes 1 and 5 of barley, Hordeum vulgare L. Soviet Genet 22:226–232
Burton RA, Jenner H, Carrangis L, Fahy B, Fincher GB, Hylton C, Laurie DA, Parker M, Waite D, van Wegen S, Verhoeven T, Denyer K (2002) Starch granule initiation and growth are altered in barley mutants that lack isoamylase activity. Plant J 31:97–112
Cook FR (2011) Control of the size and composition of the embryo in cereals. PhD dissertation, University of East Anglia
Diab AA (2006) Construction of barley consensus map showing chromosomal regions associated with economically important traits. Afr J Biotech 5:235–248
Doll H (1983) Barley seed proteins and possibilities for their improvement. In: Gottschalk W, Muller HP (eds) Seed proteins: biochemistry, genetics, nutritive value. Martinus Nijhoff, The Hague, pp 207–223
Dubreuil P, Charcosset A (1998) Genetic diversity within and among maize populations: a comparison between isozyme and nuclear RFLP loci. Theor Appl Genet 96:577–587
Fincher GB (1989) Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains. Annu Rev Plant Physiol Plant Mol Bio 40:305–346
Ishikawa N, Ishihara J, Itoh M (1995) Artificial induction and characterization of amylose-free mutants of barley. Barley Genet Newsl 24:49–53
Karakousis A, Gustafson JP, Chalmers KJ, Barr AR, Langridge P (2003) A consensus map of barley integrating SSR, RFLP and AFLP markers. Aust J Agric Res 54:1173–1185
Lorieux M (2012) MapDisto: fast and efficient computation of genetic linkage maps. Mol Breed 30:1231–1235
Mackay IJ, Caligari PDS (2000) Efficiencies of F2 and backcross generations for bulked segregant analysis using dominant markers. Crop Sci 40:626–630
Mayer KF, Waugh R, Langridge P et al (2012) A physical, genetic and functional sequence assembly of the barley genome. Nature 491:711–716
Morell MK, Kosar-Hashemi B, Cmiel M, Samuel MS, Chandler P, Rahman S, Buléon A, Batey IA, Li Z (2003) Barley sex6 mutants lack starch synthase IIa activity and contain a starch with novel properties. Plant J 34:173–185
Patron NJ, Smith AM, Fahy BF, Hylton CM, Naldrett MJ, Rossnagel BG, Denyer K (2002) The altered pattern of amylase accumulation in the endosperm of low-amylose barley cultivars is attributable to a single mutant allele of granule-bound starch synthase I with a deletion in the 5′ non-coding region. Plant Physiol 130:190–198
Patron NJ, Greber B, Fahy BF, Laurie DA, Parker ML, Denyer K (2004) The lys5 mutations of barley reveal the nature and importance of plastidial ADP-Glc transporters for starch synthesis in cereal endosperm. Plant Physiol 135:2088–2097
Wenzl P, Li H, Carling J et al (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits. BMC Genomics 7:206