Rapid induction of 7H6 tight junction-associated protein and paracellular barrier function in capillary endothelial cells of porcine brain in vitro by treatment with astrocyte conditioned medium and cAMP

Medical Electron Microscopy - Tập 30 - Trang 8-14 - 1997
Yumiko Yamada-Sasamori1, Takeo Baba2, Norimasa Sawada1, Hirotoshi Tobioka1, Hiroshi Isomura1, Kazuo Hashi2, Michio Mori1
1Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan
2Department of Neurosurgery, Sapporo Medical University, School of Medicine, Sapporo, Japan

Tóm tắt

The capillary endothelium of the central nervous system functions as a highly selective permeability barrier, corresponding to the blood-brain barrier (BBB). In the present study, we assessed the roles of the tight junction-associated proteins ZO-1 and 7H6 in the development of brain endothelial barrier function. Capillary endothelial cells (ECs) of porcine brain were cultured and treated with a combination of astrocyte conditioned medium (CM), 8-(4-chlorophenylthio) cyclic AMP (cAMP), and phosphodiesterase inhibitor. Barrier function induced within 10h was more than 10 fold higher in terms of transendothelial electrical resistance (TER) and permeability to inulin and mannitol. Concomitantly, 7H6 antigen was significantly induced at the cell border, but the expression of ZO-1 did not change significantly between the control and treated cells. These results showed the importance of astrocyte CM and cAMP for the induction of the BBB and suggested that astrocyte CM and cAMP may function differently in the induction of 7H6 antigen in brain capillary ECs.

Tài liệu tham khảo

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