Random amplified polymorphic DNA and genetic diversity in IndianMusa germplasm

Springer Science and Business Media LLC - Tập 42 Số 2 - Trang 107-118 - 1995
Bhat, K. V.1, Jarret, R. L.2
1NBPGR, Pusa Campus, New Delhi, India
2USDA/ARS, Griffin, USA

Tóm tắt

Fifty-seven accessions ofMusa including cultivated clones of 6 genomic groups (AA, AB, AAA, AAB, ABB, ABBB),M. balbisiana Colla (BB),M. acuminata Colla ssp.banksii F. Muell. (AA),M. acuminata Colla ssp.malaccensis Ridl. (AA) andM. velutina Wendl. & Drude were examined for random amplified polymorphic DNA (RAPD) genetic markers using PCR with sixty 10-mer random primers. Forty-nine of 60 tested primers gave reproducible DNA amplification patterns. The number of bands resolved per amplification was primer dependent and varied from 1 to a maximum of 24. The size range of the amplification products also differed with the selected primer sequence/genotype and ranged from 0.29 to 3.0 kb. RAPD data were used to generate Jaccard's similarity coefficients which were analyzed phenetically. Phenetic analysis separated clones into distinct groupings that were in agreement with clusterings revealed when data were subsequently analyzed by principal coordinate analysis (PCO). In both the phenetic and the PCO analyses, previously unclassified cultivars grouped with cultivars previously classified for their genomic group based on morphological keys. The implications of RAPD analysis forMusa germplasm classification, clonal identification, and management are discussed.

Tài liệu tham khảo

citation_journal_title=Genetics; citation_title=Length polymorphisms of simple sequence repeat DNA in soybean; citation_author=M. S. Akkaya, A. A. Bhagwat, P. B. Cregan; citation_volume=132; citation_publication_date=1992; citation_pages=1131-1139; citation_id=CR1 citation_journal_title=J. Hort. Sci.; citation_title=Survey of isozyme polymorphism for clonal identification inMusa. I. Esterase, acid phosphatase and catalase; citation_author=K. V. Bhat, S. R. Bhat, K. P. S. Chandel; citation_volume=67; citation_publication_date=1992; citation_pages=501-507; citation_id=CR2 citation_journal_title=J. Hort. Sci.; citation_title=Survey of isozyme polymorphism for clonal identification inMusa. II. Peroxidase, superoxide dismurase, shikimate dehydrogenase and malate dehydrogenase; citation_author=K. V. Bhat, S. R. Bhat, K. P. S. Chandel; citation_volume=67; citation_publication_date=1992; citation_pages=737-744; citation_id=CR3 citation_journal_title=HortScience; citation_title=A chemosystematic study ofMusa cultivars; citation_author=J. W. Bonner, R. M. Warner, J. L. Brewbaker; citation_volume=9; citation_publication_date=1974; citation_pages=325-328; citation_id=CR4 citation_journal_title=Anal. Biochem.; citation_title=Assay for nanogram quantities of DNA in cellular homogenates; citation_author=C. F. Brunk, K. C. Jones, T. W. James; citation_volume=92; citation_publication_date=1979; citation_pages=497-500; citation_doi=10.1016/0003-2697(79)90690-0; citation_id=CR5 citation_journal_title=Indian J. Genet.; citation_title=Origin of the cultivated bananas of Southeast Asia; citation_author=A. K. Chakravorty; citation_volume=11; citation_publication_date=1951; citation_pages=34-46; citation_id=CR6 citation_title=The Cavendish subgroup: Distinct and less distinct cultivars; citation_inbook_title=Identification of Genetic Diversity in the Genus ; citation_publication_date=1990; citation_pages=29-35; citation_id=CR7; citation_author=J. W. Daniells; citation_publisher=INIBAP citation_journal_title=Theor. Appl. Genet.; citation_title=Potential taxonomic use of random amplified polymorphic DNA (RAPD): a case study inBrassica ; citation_author=T. Demeke, R. P. Adams, R. Chibbar; citation_volume=84; citation_publication_date=1992; citation_pages=990-994; citation_doi=10.1007/BF00227415; citation_id=CR8 citation_journal_title=Euphytica; citation_title=Cytoplasmic genetic diversity in bananas and plantains; citation_author=N. Gawel, R. L. Jarret; citation_volume=52; citation_publication_date=1991; citation_pages=19-23; citation_id=CR9 citation_journal_title=Theor. Appl. Genet.; citation_title=Restriction fragment length polymorphism (RFLP)-based phylogenetic analysis ofMusa ; citation_author=N. Gawel, R.L. Jarret, A. Whittemore; citation_volume=84; citation_publication_date=1992; citation_pages=286-290; citation_doi=10.1007/BF00229484; citation_id=CR10 citation_journal_title=Phytochem.; citation_title=Distribution of anthocyanin in wild and cultivated banana varieties; citation_author=J. P. Horry, M. Jay; citation_volume=27; citation_publication_date=1988; citation_pages=2667-2672; citation_doi=10.1016/0031-9422(88)87040-7; citation_id=CR11 citation_title=Revised banana descriptors; citation_publication_date=1991; citation_id=CR12; citation_publisher=IBPGR citation_journal_title=Bull. Soc. Vaud. Sci. Nat.; citation_title=Nouvelles recherches sur la distribution florale; citation_author=P. Jaccard; citation_volume=44; citation_publication_date=1908; citation_pages=223-270; citation_id=CR13 citation_journal_title=Euphytica; citation_title=Isozymes as genetic markers in bananas and plantains; citation_author=R. L. Jarret, R. E. Litz; citation_volume=35; citation_publication_date=1986; citation_pages=539-549; citation_doi=10.1007/BF00021863; citation_id=CR14 citation_journal_title=J. Hered.; citation_title=Enzyme polymorphism inMusa acuminata Colla; citation_author=R. L. Jarret, R. E. Litz; citation_volume=77; citation_publication_date=1986; citation_pages=183-186; citation_id=CR15 citation_journal_title=Theor. Appl. Genet.; citation_title=RFLP-based phylogeny ofMusa species in Papua New Guinea; citation_author=R. L. Jarret, N. Gawel, A. Whittemore; citation_volume=84; citation_publication_date=1992; citation_pages=579-584; citation_doi=10.1007/BF00224155; citation_id=CR16 citation_journal_title=Euphytica; citation_title=Detecting genetic diversity in diploid bananas using PCR and primers from a highly repetitive DNA sequence; citation_author=R. L. Jarret, D. Vuylsteke, N. Gawel, R. Pimentel, L. Dunbar; citation_volume=68; citation_publication_date=1993; citation_pages=69-76; citation_doi=10.1007/BF00024156; citation_id=CR17 citation_journal_title=BioTechnology; citation_title=Oligonucleotide and amplification fingerprinting of wild species and cultivars of banana (Musa spp.); citation_author=D. Kaemmer, A. Afza, K. Weising, G. Kahl, F. J. Novak; citation_volume=10; citation_publication_date=1993; citation_pages=1030-1035; citation_id=CR18 citation_journal_title=Theor. Appl. Genet.; citation_title=Identification of apple cultivars using RAPD markers; citation_author=B. Koller, A. Lehmann, J. M. McDermott, C. Gessler; citation_volume=85; citation_publication_date=1993; citation_pages=901-904; citation_doi=10.1007/BF00225036; citation_id=CR19 citation_journal_title=Heredity; citation_title=Variation of ribosomal gene spacer length among wild and cultivated bananas; citation_author=C. Lanaud, H. Tezenas du Montcel, M. P. Jolivot, J. C. Glaszmann, D. Gonzalez de Leon; citation_volume=68; citation_publication_date=1992; citation_pages=147-156; citation_id=CR20 citation_journal_title=Euphytica; citation_title=Genetic relationships among cultivated bananas and plantains from Asia and the Pacific; citation_author=V. Lebot, K. M. Aradhya, R. Manshardt, B. Meilleur; citation_volume=67; citation_publication_date=1993; citation_pages=163-176; citation_doi=10.1007/BF00040618; citation_id=CR21 citation_journal_title=PCR: Methods and Applications; citation_title=The random amplification of polymorphic DNA for fingerprinting plants; citation_author=M. Munthali, B. Ford-Lloyd, H. J. Newbury; citation_volume=1; citation_publication_date=1992; citation_pages=274-276; citation_id=CR22 citation_journal_title=Scientia. Hortic.; citation_title=The use of random amplified polymorphic DNAs to fingerprint apple genotypes; citation_author=D. L. Mulcahy, M. Cresti, S. Sansavini, G. C. Douglas, H. F. Linskens, G. B. Mulcahy, R. Vignani, M. Pancaldi; citation_volume=54; citation_publication_date=1993; citation_pages=89-96; citation_doi=10.1016/0304-4238(93)90057-W; citation_id=CR23 citation_journal_title=Plant Growth Reg.; citation_title=The use of RAPD for assessing variation in plants; citation_author=H. J. Newbury, B. Ford-Lloyd; citation_volume=12; citation_publication_date=1993; citation_pages=43-51; citation_doi=10.1007/BF00144581; citation_id=CR24 citation_journal_title=Proc. Natl. Acad. Sci. (USA); citation_title=Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics; citation_author=M. A. Saghai-Maroof, K. M. Soliman, R. A. Jorgenson, R. W. Allard; citation_volume=81; citation_publication_date=1984; citation_pages=8014-8018; citation_doi=10.1073/pnas.81.24.8014; citation_id=CR25 Sambrook, J., E. F. Fritsch & T. Maniatis, 1989. Molecular cloning — a laboratory manual. 2nd ed., Cold Spring Harbor, NY, USA. citation_title=Banana taxonomy, breeding and production technology; citation_publication_date=1992; citation_id=CR27; citation_author=K. G. Shanmugavelu; citation_author=K. Aravindakshan; citation_author=S. Sathiamoorthy; citation_publisher=Metropolitan Book Co. citation_title=Collecting in Papua New Guinea; citation_inbook_title=Identification of genetic diversity in the genus ; citation_publication_date=1990; citation_pages=140-157; citation_id=CR28; citation_author=S. Sharrock; citation_publisher=INIBAP Montpellier citation_journal_title=Pacific Sci.; citation_title=Notes on banana varieties in Hawaii; citation_author=N. W. Simmonds; citation_volume=8; citation_publication_date=1954; citation_pages=226-229; citation_id=CR29 citation_journal_title=J. Hort. Sci.; citation_title=Varietal identification in the Cavendish subgroup of bananas; citation_author=N. W. Simmonds; citation_volume=29; citation_publication_date=1954; citation_pages=81-87; citation_id=CR30 citation_title=The evolution of the bananas; citation_publication_date=1962; citation_id=CR31; citation_author=N. W. Simmonds; citation_publisher=Longman citation_title=Bananas; citation_publication_date=1966; citation_id=CR32; citation_author=N. W. Simmonds; citation_publisher=Longman citation_journal_title=J. Linn. Soc. (Bot.) London.; citation_title=The taxonomy and origins of the cultivated bananas; citation_author=N. W. Simmonds, K. Shepherd; citation_volume=55; citation_publication_date=1955; citation_pages=302-312; citation_doi=10.1111/j.1095-8339.1955.tb00015.x; citation_id=CR33 citation_journal_title=Theor. Appl. Genet.; citation_title=Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars; citation_author=J. I. Stiles, C. Lemme, S. Sonder, M. B. Morshidi, R. Manshardt; citation_volume=85; citation_publication_date=1993; citation_pages=697-701; citation_doi=10.1007/BF00225007; citation_id=CR34 citation_title=Bananas; citation_publication_date=1989; citation_id=CR35; citation_author=R. H. Stover; citation_author=N. W. Simmonds; citation_publisher=Longman citation_title=Limits of morphotaxonomy: Names and synonyms of plantain in Africa and elsewhere; citation_inbook_title=Identification of Genetic Diversity in the Genus ; citation_publication_date=1990; citation_pages=172-210; citation_id=CR36; citation_author=R. Swennen; citation_publisher=INIBAP citation_title= subspecies: Status and diversity; citation_inbook_title=Identification of Genetic Diversity in the Genus ; citation_publication_date=1990; citation_pages=211-218; citation_id=CR37; citation_author=H. Tezenas du Montcel; citation_publisher=INIBAP citation_journal_title=IITA Research; citation_title=Somaclonal variation in African plantains; citation_author=D. Vuylsteke, R. Swennen; citation_volume=1; citation_publication_date=1990; citation_pages=4-10; citation_id=CR38 citation_journal_title=Electrophoresis; citation_title=Plant DNA fingerprinting with radioactive and digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences; citation_author=K. Weising, B. Beyermann, J. Ramser, G. Kahl; citation_volume=12; citation_publication_date=1991; citation_pages=159-169; citation_doi=10.1002/elps.1150120211; citation_id=CR39 citation_journal_title=Theor. Appl. Genet.; citation_title=Identification and classification of celery cultivars with RAPD markers; citation_author=X. Yang, C. Quiros; citation_volume=86; citation_publication_date=1993; citation_pages=205-212; citation_doi=10.1007/BF00222080; citation_id=CR40