Radiation Therapy Reduced Blood Levels of LDH, HIF-1α, and miR-210 in OSCC

Springer Science and Business Media LLC - Tập 26 - Trang 433-442 - 2018
Marcela Gonçalves de Souza1, Sabrina Ferreira de Jesus1, Eloá Mangabeira Santos1, Emisael Stenio Batista Gomes1, Arlen de Paulo Santiago Filho2, Eliane Macedo Sobrinho Santos3, Luiz Henrique da Silveira1, Sérgio Henrique Sousa Santos4, Alfredo Maurício Batista de Paula1, Lucyana Conceição Farias1, André Luiz Sena Guimarães1,2,5
1Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil
2Hospital Dilson Godinho, Montes Claros, Brazil
3Instituto Federal de Educação, Ciência e Tecnologia do Norte de Minas Gerais (IFNMG), Araçuaí, Brazil
4Institute of Agricultural Sciences. Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Brazil
5Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, Montes Claros, Brazil

Tóm tắt

Radiation Therapy (RT) is a treatment option for a large number of neoplasias. However, the effect of RT on the level of hypoxia markers is poorly understood. The present study aimed to investigate the effect of RT on the levels of hypoxic markers in Oral squamous cell carcinoma (OSCC). Evaluation of HIF-1α and miR-210 levels in OSCC was performed. Then a proteomic analysis was performed to identify candidate hypoxic targets of RT. To validate proteomic studies, the effect of RT on HIF-1α, miR-210, PDH-A and LDH-A levels under hypoxia was assessed by qRT-PCR. The impact of RT in hypoxia markers was evaluated in patients to confirm in vitro results. An increase in the HIF-1α levels was observed in OSCC. RT reduced OSCC cell proliferation and migration. Interestingly, hypoxia could revert the effect of radiation on OSCC phenotype. However, proteomics analyses suggested that LDH is one of the critical targets of RT even in hypoxia. Moreover, RT decreased HIF-1α, miR-210, and LDH even in hypoxia. The current study demonstrated that hypoxia could revert the effects of RT in the OSCC context. However, RT reduces the levels HIF-1α, miR-210 and LDH in vivo and in vitro. The consequences of RT in blood should be carefully investigated.

Tài liệu tham khảo

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