Quantitative Trait Loci Analysis of Endosperm Color and Carotenoid Content in Sorghum Grain

Wiley - Tập 48 Số 5 - Trang 1732-1743 - 2008
Maria G. Salas Fernandez1, Martha T. Hamblin2, Li Li3, William L. Rooney4, Mitchell R. Tuinstra5, Stephen Kresovich6
1157 Biotechnology Bldg., Dep. of Plant Breeding and Genetics Cornell Univ. Ithaca NY 14853
2Institute for Genomic Diversity, 156 Biotechnology Bldg. Cornell Univ. Ithaca NY 14853
3USDA-ARS, Plant, Soil, and Nutrition Lab., Dep. of Plant Breeding and Genetics, Cornell Univ., Ithaca, NY 14853
4Dep. of Soil & Crop Science Texas A&M Univ. 2474 TAMU College Station TX 77843‐2474
5Dep. of Agronomy, 2004A Throckmorton Hall Kansas State Univ. Manhattan KS 66506
6Institute for Genomic Diversity, 158 Biotechnology Bldg. Cornell Univ. Ithaca NY 14853

Tóm tắt

Vitamin A deficiency affects approximately 250 million people in semiarid regions of Africa and Asia, where sorghum (Sorghum bicolor Moench) is a major staple crop. Yellow endosperm sorghums contain carotenoids, some of which can be transformed by humans into vitamin A. Our objective was to study the genetic basis of variation in carotenoid levels in sorghum endosperm by mapping quantitative trait loci (QTL) associated with carotenoid content and endosperm color, as a putative predictor of carotenoid concentration. A recombinant inbred line population developed from a yellow (‘KS115’) by a white endosperm (‘Macia’) parental cross was evaluated in two locations in 2005. A genetic map was generated using 112 molecular markers including nine carotenoid candidate genes. Lutein, zeaxanthin, and β‐carotene were the major carotenoids identified. Several QTL were detected for each compound as well as for color and total carotenoids. Color was significantly correlated with the levels of all compounds, and color QTL co‐localized with carotenoid QTL. For β‐carotene (provitamin A), five QTL were localized on chromosomes 1, 2, and 10. One of them, on chromosome 2, was stable across both environments, had positive additive effects (1.179 and 1.379), explained large proportions of the phenotypic variance (11.6% and 15.15%), and was associated with a new phytoene synthase gene (Psy3). This first report of QTL for carotenoid content in sorghum grain provides a starting point for breeding high‐provitamin A sorghums.

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Wiley

Tập 48 Số 5

1732-1743

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