Quantitative PCR for counting residual white blood cells in blood products

Fournel JJ, Zingsem J, Riggert J, Muylle L, Müller N, Köhler M, Beaumont JL, Baeten M, Eckstein R, van Waeg G. A multicenter evaluation of the routine use of a new white cell-reduction apheresis system for collection of platelets. Transfusion 1997;37:487–92 Riggert J, Schwartz DW, Wieding JU, Mayr WR, Köhler M. Prestorage inline filtration of whole blood for obtaining white cell-reduced blood components. Transfusion 1997;37:1039–1044 Dzik S. Principles of counting low numbers of leukocytes in leukoreduced blood components. Transfus Med Rev 1997;11:44–55 Rebulla P, Dzik WH. Multicenter evaluation of methods for counting residual white cells in leukocyte-depleted red blood cells. The Biomedical Excellence for Safer Transfusion (BEST) Working Party of the International Society of Blood Transfusion. Vox Sang 1994;66:25–32 Allain JP. The status of hepatitis C virus screening. Transfus Med Rev 1998;12:46–55 Prati D, Rawal BD, Dang C, Capelli C, Vyas GN. DNA enzyme immunoassay of the PCR-amplified HLA-DQ alpha gene for estimating residual leukocytes in filtered blood. Clin Diagn Lab Immunol 1995;2:182–85 Lee TH, Stromberg RR, Heitman J, Tran K, Busch MP. Quantitation of residual white cells in filtered blood components by polymerase chain reaction amplification of HLA DQ-A DNA. Transfusion 1994;34:986–94 Legler TJ, Köhler M, Mayr WR, Panzer S, Ohto H, Fischer GF. Genotyping of the human platelet antigen systems 1 through 5 by multiplex polymerase chain reaction and ligation-based typing. Transfusion 1996;36:426–31 White TJ, Madej R, Persing DH. The polymerase chain reaction: clinical applications. Adv Clin Chem 1992;29:161–196 Morris T, Robertson B, Gallagher M. Rapid reverse transcription-PCR detection of hepatitis C virus RNA in serum by using the TaqMan fluorogenic detection system. J Clin Microbiol 1996;34:2933–936