Quantitative Analysis of Colistin-Resistant Escherichia coli in Retail Meat from Local Vietnamese Markets

BioMed Research International - Tập 2021 - Trang 1-6 - 2021
Thắng Nguyễn1, Diep Thi Khong1, Ha Vu Le1, Hoa Tran1, Phan Ngoc Quang1, Huong Thi Thu Le1, Ryuji Kawahara2, Yoshimasa Yamamoto3
1Center of Medical-Pharmaceutical Science and Technology Services, Thai Binh University of Medicine and Pharmacy, Thai Binh, Vietnam
2Department of Microbiology, Osaka Institute of Public Health, Osaka 537-0025, Japan
3Life Science Research Center, Gifu University, Gifu 501-1194, Japan

Tóm tắt

The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the quantitative level of resistant bacteria in food, has not yet been well elucidated. In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene, mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median quantitative levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 10 4 and 4.2 × 10 3  CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli was a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

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BioMed Research International

Tập 2021

1-6

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