Qualitative and Quantitative Analyses of Flavonoids in Spirodela polyrrhiza by High‐performance Liquid Chromatography Coupled with Mass Spectrometry

Phytochemical Analysis - Tập 22 Số 6 - Trang 475-483 - 2011
Xue Qiao1, Wenni He2,1, Xiang Cheng1, Jian Han1, WU Li-jun2, De‐an Guo1, Yi Kuang1
1The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China
2School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China

Tóm tắt

ABSTRACTIntroductionSpirodela polyrrhiza (L.) Schleid. is a traditional Chinese herbal medicine for the treatment of influenza. Despite its wide use in Chinese medicine, no report on quality control of this herb is available so far.ObjectiveTo establish qualitative and quantitative analytical methods by high‐performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) for the quality control of S. polyrrhiza.MethodologyThe methanol extract of S. polyrrhiza was analysed by HPLC/ESI‐MSn. Flavonoids were identified by comparing with reference standards or according to their MSn (n = 2–4) fragmentation behaviours. Based on LC/MS data, a standardised HPLC fingerprint was established by analysing 15 batches of commercial herbal samples. Furthermore, quantitative analysis was conducted by determining five major flavonoids, namely luteolin 8‐C‐glucoside, apigenin 8‐C‐glucoside, luteolin 7‐O‐glucoside, apigenin 7‐O‐glucoside and luteolin.ResultsA total of 18 flavonoids were identified by LC/MS, and 14 of them were reported from this herb for the first time. The HPLC fingerprints contained 10 common peaks, and could differentiate good quality batches from counterfeits. The total contents of five major flavonoids in S. polyrrhiza varied significantly from 4.28 to 19.87 mg/g.ConclusionQualitative LC/MS and quantitative HPLC analytical methods were established for the comprehensive quality control of S. polyrrhiza. Copyright © 2011 John Wiley & Sons, Ltd.

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Phytochemical Analysis

Tập 22 Số 6

475-483

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