QTL mapping for germination of seeds obtained from previous wheat generation under drought

Walter de Gruyter GmbH - 2014
Ilona Czyczyło-Mysza1, Izabela Marcińska1, Edyta Skrzypek1, Katarzyna Cyganek1, Katarzyna Juzoń1, Małgorzata Karbarz
11The F. Górski Institute of Plant Physiology, Polish Academy of Sciences, 30-239, Kraków, Poland

Tóm tắt

AbstractThe QTLs controlling germination and early seedling growth were mapped using seeds acquired from mapping population and parental lines of Chinese Spring and SQ1 grown under water-limited conditions, severe drought (SDr) and well-watered plants (C). Germination ability was determined by performing a standard germination test based on the quantification of the germination percentage (GP24) of seeds incubated for 24 h at 25°C in the dark. Early seedling growth was evaluated on the basis of the length of the root and leaf at the 6th day of the experiment. QTLs were identified by composite interval mapping method using Windows QTLCartographer 2.5 software. For the traits studied, a total of thirty eight additive QTLs were identified. Seventeen QTLs were mapped in C on chromosomes: 1A, 2A, 7A, 1B, 2B, 3B, 4B, 5B, 6B, 7B, 2D, 3D, 4D and 6D, while twenty one QTLs were identified in SDr on chromosomes: 1A, 2A, 5A, 2B, 3B, 4B, 5B, 6B, 7B, 3D, 5D and 6D. Most of the QTLs for GP and early leaf growth parameters were clustered on chromosome 4B (associated with the Rht-B1 marker) both in C and SDr plants. The results indicate the complex and polygenic nature of germination.

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Tài liệu tham khảo

Paterson, 1991, DNA markers in plant improvement http dx org, Adv Agron, 38, 39, 10.1016/S0065-2113(08)60578-7

CzyczyŁo, 2013, Quantitative trait loci for leaf chlorophyll fluorescence parameters chlorophyll and carotenoid contents in relation to biomass and yield in bread wheat and their chromosome deletion bin http dx org, Mol Breed, 24, 189, 10.1007/s11032-013-9862-8

Rebetzke, 2007, Molecular mapping of genes for coleoptile growth in bread wheat ( Triticum aestivum http dx org, Theor Appl Genet, 17, 1, 10.1007/s00122-007-0509-1

Dekkers, 2002, The use of molecular genetics in the improvement of agricultural populations http dx org, Nat Rev Genet, 40, 22, 10.1038/nrg701

Young, 1999, A cautiously optimistic vision for marker - assisted selection http dx org, Mol Breed, 39, 505, 10.1023/A:1009684409326

Edney, 2004, Quantitative trait loci affecting germination traits and malt friability in a two - rowed by six - rowed barley cross http dx org, Cereal Sci, 12, 283, 10.1016/j.jcs.2003.10.008

Marcińska, 2013, Impact of osmotic stress on physiological and biochemical characteristics in drought susceptible and drought resistant wheat genotypes http dx org, Acta Physiol Plant, 26, 451, 10.1007/s11738-012-1088-6

Landjeva, 2010, Genetic mapping within the wheat D genome reveals QTL for germination seed vigour and longevity and early seedling growth http dx org, Euphytica, 45, 129, 10.1007/s10681-009-0016-3

Bultynck, 2004, ter From individual leaf elongation to whole shoot leaf area expansion ; a comparison of three Aegilops and two Triticum species http dx org, Ann Bot, 42, 99, 10.1093/aob/mch110

Börner, 2002, Mapping of quantitative trait loci determining agronomically important characters in hexaploid wheat ( Triticum aestivum http dx org, Theor Appl Genet, 47, 921, 10.1007/s00122-002-0994-1

Bettey, 2000, Quantitative genetic analysis of seed vigor and pre - emergence seedling growth traits in Brassica oleracea http dx org, New Phytol, 7, 148, 10.1046/j.1469-8137.2000.00760.x

Quarrie, 2005, A high density genetic map of hexaploid wheat ( Triticum aestivum from the cross Chinese Spring SQ and its use to compare QTLs for grain yield across a range of environments http dx org, Theor Appl Genet, 23, 865, 10.1007/s00122-004-1902-7

Tranbarger, 2000, Regulation of NADPH - cytochrome reductase expressed during Douglas - fir germination and seedling development http dx org, Plant Mol Biol, 43, 450, 10.1023/A:1006425025702

Hrstková, 2006, Estimation of barley seed vigour with respect to variety and provenance effects, Genet Plant Breed, 34, 44, 10.17221/3653-CJGPB

Landjeva, 2008, Molecular mapping of genomic regions associated with growth response to osmotic stress in wheat seedling http dx org, Biol Plant, 10, 259, 10.1007/s10535-008-0056-x

Groos, 2003, Genetic analysis of grain protein - content grain yield and thousand - kernel weight in bread wheat, Theor Appl Genet, 48, 1032, 10.1007/s00122-002-1111-1

Stefani, 2000, Low temperature storage of caryopses of Triticum durum : viability and longevity http dx org, Ann Bot, 33, 403, 10.1006/anbo.1999.1050

Collard, 2005, An introduction to markers quantitative trait loci QTL mapping and marker - assisted selection for crop improvement : the basic concepts http dx org, Euphytica, 9, 142, 10.1007/s10681-005-1681-5

Rebetzke, 2004, Genetic analysis of coleoptile length and diameter of wheat http dx org, Agric Res, 15, 733, 10.1071/AR04037

Habash, 2007, The genetics of nitrogen use in hexaploid wheat : N utilisation development and yield http dx org, Theor Appl Genet, 50, 403, 10.1007/s00122-006-0429-5

Angaji, 2008, Mapping QTLs for submergence tolerance during germination in rice, Afr J Biotechnol, 19, 2551

Cui, 2002, Molecular dissection of seedling - vigor and associated physiological traits in rice http dx org, Theor Appl Genet, 11, 745, 10.1007/s00122-002-0908-2

Zhang, 2005, Mapping quantitative trait loci QTLs ) for seedling - vigor using recombinant inbred lines of rice ( Oryza sativa Field http dx org, Crop Res, 8, 161, 10.1016/j.fcr.2004.06.004

Limami, 2002, Genetic and physiological analysis of germination efficiency in maize in relation to nitrogen metabolism reveals the importance of cytosolic glutamine synthetase http dx org, Plant Physiol, 22, 130, 10.1104/pp.009647

Imai, 2004, Spermidine synthase genes are essential for survival of Arabidopsis http dx org, Plant Physiol, 44, 135, 10.1104/pp.104.041699

Aparicio, 2002, Seedling development and biomass as affected by seed size and morphology in durum wheat http dx org, Agric Sci, 35, 139, 10.1017/S0021859602002411

Al Chaarani, 2005, Identification of QTLs for germination and seeding development in sunflower ( Helianthus annus http dx org, Plant Sci, 13, 169, 10.1016/j.plantsci.2005.03.016

Rebetzke, 1999, Breeding long coleoptile reduced height wheats http dx org, Euphytica, 14, 159, 10.1023/A:1003518920119

Pieta Filho, 1991, The development of seed quality in spring barley in four environments Germination and longevity, Seed Sci Res, 31, 1

ter Steege, 2005, den Genetic and physiological architecture of early vigor in Aegilops tauschii the D - genome donor of hexaploid wheat A quantitative trait loci analysis http dx org, Plant Physiol, 30, 1078, 10.1104/pp.105.063263

Dubcovsky, 2004, Marker - assisted selection in public breeding programs : the wheat experience http dx org, Crop Sci, 37, 1895, 10.2135/cropsci2004.1895

Baretto Dias, 2011, QTL analysis of seed germination and pre - emergence growth at extreme temperatures in Medicago truncatula http dx org, Theor Appl Genet, 20, 122, 10.1007/s00122-010-1458-7

Zagdańska, 1997, Mechanisms of grains resistance to soil drought : energy metabolism of spring wheat in the acquisition of resistance [ Mechanizmy odporności zbóż na suszę glebową : metabolizm energetyczny pszenicy jarej w nabywaniu odporności in Polish, IHAR, 2, 203

Foolad, 1999, Comparison of QTLs for seed germination under non - stress cold stress and salt stress in tomato Plant http dx org, Breed, 21, 167, 10.1046/j.1439-0523.1999.118002167.x

Quarrie, 2006, Dissecting a wheat QTL for yield present in a range of environments : from the QTL to candidate genes http dx org, Exp Bot, 46, 2627, 10.1093/jxb/erl026

Rebetzke, 2007, Genotypic increase in coleoptiles length improves wheat establishment early vigour and grain yield with deep sowing Field http dx org, Crop Res, 16, 10, 10.1016/j.fcr.2006.05.001

Villar, 1998, Relative growth rate and biomass allocation in Aegilops species http dx org, New Phytol, 41, 425, 10.1046/j.1469-8137.1998.00286.x

Eagles, 2002, Estimation and utilization of glutenin gene effects from the analysis of unbalanced data from wheat breeding programs http dx org, Agric Res, 36, 367, 10.1071/AR01074

Nawroz Abdul, 2010, Germination characteristics and molecular characterization of some wheat varieties in Sulaimanyah by SSR Marker of Biology, Turkish Journal, 18, 109

McCartney, 2005, Mapping quantitative trait loci controlling agronomic traits in the spring wheat cross RL AC Domain http dx org, Genome, 49, 870, 10.1139/g05-055

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