Pyrene actin: documentation of the validity of a sensitive assay for actin polymerization

John A. Cooper1, Simon B. Walker1, Thomas D. Pollard1
1Department of Cell Biology & Anatomy, Johns Hopkins University School of Medicine, Baltimore, USA

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BONDER, E. M. & MOOSEKER, M. S. (1981) The acrosomal process: a new tool in studying actin assembly and structure.J. Cell Biol. 91, 306a.

BRADFORD, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analyt. Biochem. 72, 248?54.

COOPER, J. A. & POLLARD, T. D. (1982) Methods to measure actin polymerization.Meth. Enzymol. 85, 182?210.

CRAIG, S. W. & POWELL, L. D. (1980) Regulation of actin polymerization by villin, a 95 000 dalton cytoskeleton component of intestinal brush border.Cell 22, 739?46.

DETMERS, P., WEBER, A. ELZINGA, M. & STEPHENS, R. E. (1981) 7-Chloro-4-nitrobenzeno-2-oxa-1,3-diazole actin as a probe for actin polymerization.J. biol. Chem. 256, 99?105.

HIGASHI, S. & OOSAWA, F. (1965) Conformational changes associated with polymerization and nucleotide binding in actin molecules.J. molec. Biol. 12, 843?65.

HOUK, T. W., JR & UE, K. (1974) The measurement of actin concentration in solution: a comparison of methods.Analyt. Biochem. 62, 66?74.

KOUYAMA, T. & MIHASHI, K. (1981) Fluorimetry study ofN-(1-pyrenyl)iodoacetamide-labelled F-actin.Eur. J. Biochem. 114, 33?8.

KORN, E. D. (1982) Actin polymerization and its regulation by proteins from non-muscle cells.Physiol. Rev. 62, 672?737.

LEE, S., COOPER, J. A. & POLLARD, T. D. (1982) Fluorescence measurement of Acanthamoeba profilin binding to pyrene-labeled actin monomers.J. Cell Biol. 95, 297a.

LOWRY, O. H., ROSENBROUGH, N. J., FARR, A. L. & RANDALL, R. J. (1951) Protein measurement with the Folin phenol reagent.J. biol. Chem. 193, 265?75.

OOSAWA, F. & ASAKURA, S. (1975)Thermodynamics of the Polymerization of Protein. New York: Academic Press.

POLLARD, T. D. & CRAIG, S. W. (1982) Mechanism of actin polymerization.TIBS 7, 55?8.

POLLARD, T. D. & MOOSEKER, M. S. (1981) Direct measurement of actin polymerization rate constants by electron microscopy of actin filaments nucleated by isolated microvillus cores.J. Cell Biol. 88, 654?9.

TAYLOR, D. L., REIDLER, J. SPUDICH, J. & STRYER, L. (1981) Detection of actin assembly by fluorescence energy transfer.J. Cell Biol. 89, 362?7.

TELLAM, R. & FRIEDEN, C. (1982) Cytochalasin D and platelet gelsolin accelerate actin polymer formation. A model for regulation of the extent of actin polymer formationin vivo.Biochemistry 21, 3207?14.

TILNEY, L. G., BONDER, E. M. & DEROSIER, D. J. (1981) Actin filaments elongate from their membrane-associated ends.J. Cell Biol. 90, 485?94.

TSENG, P. & POLLARD, T. D. (1982) Mechanism of action ofAcanthamoeba profilin.J. Cell Biol. 94, 213?8.

WALSH, T. P. & WEGNER, A. (1980) Effect of the state of oxidation of cysteine 190 of tropomyosin on the assembly of the actin-tropomyosin complex.Biochim. Biophys. Acta 626, 79?87.

WEGNER, A. (1976) Head to tail polymerization of actin.J. molec. Biol. 108, 139?50.

WEGNER, A. & ENGEL, J. (1975) Kinetics of the cooperative association of actin to actin filaments.Biophys. Chem. 3, 215?25.