Purification, sequence, and cellular localization of a novel chromosomal protein that binds to Methylated DNA

Cell - Tập 69 Số 6 - Trang 905-914 - 1992
Joe Lewis1,2, Richard R. Meehan1,2, William J. Henzel3, Ingrid Maurer-Fogy4, Peter Jeppesen5, Franz Klein6, Adrian Bird1,2
1Institute of Cell and Molecular Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR. Scotland
2Research Institute of Molecular Pathology, Dr. Bohr Gasse 7, 1030 Vienna, Austria
3Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California 94080, USA
4Ernst Boehringer Institute Dr. Boehringer Gasse 5–11 A-1121 Vienna Austria
5Medical Research Council, Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, Scotland
6Botanisches Institut der Universitat Rennweg 14 1030 Vienna Austria

Tóm tắt

Từ khóa


Tài liệu tham khảo

Antequera, 1989, Specific protection of methylated CpGs in mammalian nuclei, Cell, 58, 509, 10.1016/0092-8674(89)90431-5

Antequera, 1990, High levels of de novo methylation and altered chromatin structure at CpG islands in cell lines, Cell, 62, 503, 10.1016/0092-8674(90)90015-7

Ashley, 1989, Isolation and sequencing of cDNA clones encoding Drosophila chromosomal protein D1, J. Biol. Chem., 264, 8394, 10.1016/S0021-9258(18)83195-7

Bell, 1991, Physical mapping across the fragile X: hypermethylation and clinical expression of the fragile X syndrome, Cell, 64, 861, 10.1016/0092-8674(91)90514-Y

Bird, 1986, CpG rich islands and the function of DNA methylation, Nature, 321, 209, 10.1038/321209a0

Boyes, 1991, DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein, Cell, 64, 1123, 10.1016/0092-8674(91)90267-3

Boyes, 1992, Repression of genes by DNA methylation depends upon CpG density and promoter strength: evidence for involvement of a methyl-CpG binding protein, EMBO J., 11, 327, 10.1002/j.1460-2075.1992.tb05055.x

Buschhausen, 1985, Inhibition of herpes simplex thymidine kinase gene expression by DNA methylation is an indirect effect, Nucl. Acids Res., 13, 5503, 10.1093/nar/13.15.5503

Churchill, 1989, “SPKK” motifs prefer to bind to DNA at A/T-rich sites, EMBO J., 8, 4189, 10.1002/j.1460-2075.1989.tb08604.x

Comings, 1973, The mechanism of C and G banding of chromosomes, Exp. Cell Res., 77, 469493, 10.1016/0014-4827(73)90601-0

Gorski, 1986, Tissue-specific in vitro transcription from the mouse albumin promoter, Cell, 47, 767, 10.1016/0092-8674(86)90519-2

Groudine, 1981, Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation, Nature, 292, 311, 10.1038/292311a0

Henikoff, 1984, Unidirectional digestion with exonuclease III creates targetted breakpoints for DNA sequencing, Gene, 28, 351, 10.1016/0378-1119(84)90153-7

Hojoh, 1990, Selective cloning and sequence analysis of the human Lt (LINE-1) sequences which transposed in the relatively recent past, Nucl. Acids Res., 18, 4099, 10.1093/nar/18.14.4099

Horz, 1981, Nucleotide sequence of mouse satellite DNA, Nucl. Acids Res., 9, 683, 10.1093/nar/9.3.683

Hubscher, 1987, Double replica Southwestern, Nucl. Acids Res., 15, 5486, 10.1093/nar/15.13.5486

Jeppesen, 1991, Antibodies to defined histone epitopes reveal variations in chromatin conformation and underacetylation of centric heterochromatin in human metaphase chromosomes, Chromosome, 101, 322, 10.1007/BF00346011

Johnson, 1989, Alternative processing of mRNAs encoding mammalian chromosomal high mobility group proteins HMG I and HMG Y, Mol. Cell. Biol., 9, 2114, 10.1128/MCB.9.5.2114

Jones, 1970, Chromosomal and nuclear location of mouse satellite DNA in individual cells, Nature, 225, 912, 10.1038/225912a0

Joseph, 1989, The organization of the mouse satellite DNA at centromeres, Exp. Cell Res., 183, 494, 10.1016/0014-4827(89)90408-4

Keshet, 1985, Effect of regional DNA methylation on gene expression, 82, 2560

Kit, 1961, Equilibrium centrifugation in density gradients of DNA preparations from animal cells, J. Mol. Biol., 3, 711, 10.1016/S0022-2836(61)80075-2

Laemmli, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0

Levine, 1991, Inhibition of promoter activity by methylation: possible involvement of protein mediators, 88, 6515

Manson, 1981, An examination of the relative resistances to aflatoxin 131 and susceptibilities to gamma-glutamyl and p-phenylene diamine mustard of gammaglutamyl transferase negative and positive cell lines, Carcinogenesis, 2, 661, 10.1093/carcin/2.7.661

Manuelidis, 1981, Consensus sequence of mouse satellite DNA indicates it is derived from tandem 116 base pair repeats, FEBS Lett., 129, 25, 10.1016/0014-5793(81)80746-6

Matsudaira, 1987, Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes, J. Biol. Chem., 262, 10035, 10.1016/S0021-9258(18)61070-1

Meehan, 1989, Identification of a mammalian protein that binds specifically to DNA containing methylated CpGs, Cell, 58, 499, 10.1016/0092-8674(89)90430-3

Miller, 1974, 5-Methylcytosine localised in mammalian constitutive heterochromatin, Nature, 251, 636, 10.1038/251636a0

Miskimins, 1985, Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene, 32, 6741

Murray, 1987, Site specific demethylation in the promoter of human gamma globin gene does not alleviate methylation mediated suppression, EMBO J., 6, 2329, 10.1002/j.1460-2075.1987.tb02508.x

Oberlé, 1991, Instability of a 550-base pair DNA segment and abnormal methylation in fragile X syndrome, Science, 252, 1097, 10.1126/science.252.5009.1097

Pardue, 1970, Chromosomal localization of mouse satellite DNA, Science, 168, 1356, 10.1126/science.168.3937.1356

Pawlak, 1991, An avian 40 kd nucleoprotein binding preferentially to a promoter sequence containing one single pair of methylated CpG, Nucl. Acids Res., 19, 1029, 10.1093/nar/19.5.1029

Pfeifer, 1990, In vivo footprint and methylation analysis by PCR-aided genomic sequencing: comparison of active and inactive X chromosomal DNA at the CpG island and promoter of human pgk-1, Genes Dev., 4, 1277, 10.1101/gad.4.8.1277

Reeves, 1990, The AT-DNA-binding domain of mammalian high mobility group I chromosomal proteins, J. Biol. Chem., 265, 8573, 10.1016/S0021-9258(19)38926-4

Renbaum, 1990, Cloning, characterization, and expression in Escherichia coli of the gene coding for the CpG DNA methylase from Spiroplasma sp. strain MQ1(M. Sssl), Nucl. Acids Res., 18, 1145, 10.1093/nar/18.5.1145

Rickwood, 1975, Improved techniques for the fractionation of non-histone proteins of chromatin on hydroxyapatite, Eur. J. Biochem., 51, 593, 10.1111/j.1432-1033.1975.tb03961.x

Rigby, 1977, Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase, J. Mol. Biol., 113, 237, 10.1016/0022-2836(77)90052-3

Sanger, 1977, DNA sequencing with chain terminating inhibitors, 74, 5463

Schmid, 1984, 5-Azacytidine-induced undercondensations in human chromosomes, Hum. Genet., 67, 257, 10.1007/BF00291352

Solage, 1978, Organization of 5-methylcytosine in chromosomal DNA, Biochemistry, 17, 2934, 10.1021/bi00607a036

Stanley, 1984, Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins, EMBO J., 3, 1429, 10.1002/j.1460-2075.1984.tb01988.x

Stein, 1982, In vitro methylation of the hamster adenine phosphoribosyltransferase gene inhibits its expression in mouse L cells, 79, 4418

Stewart, 1982, De novo methylation and infectivity of retroviral genomes introduced into embryonal carcinoma cells, 79, 4098

Sutter, 1980, Methylation of integrated adenovirus type 12 DNA sequences in transformed cells is inversely correlated with viral gene expression, 77, 253

van der Ploeg, 1980, DNA methylation in the human γσβ-globin locus in erythroid and nonerythroid tissues, Cell, 19, 947, 10.1016/0092-8674(80)90086-0

Viegas-Pequignot, 1976, Segmentation of human chromosomes induced by 5-ACR (5-azacytidine), Hum. Genet., 34, 247, 10.1007/BF00295287

Vinson, 1988, In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage, Genes Dev., 2, 801, 10.1101/gad.2.7.801

Wang, 1986, Methylated DNA-binding protein from human placenta recognizes specific methylated sites on several prokaryotic DNAs, Nucl. Acids Res., 14, 9843, 10.1093/nar/14.24.9843

Weih, 1991, Analysis of CpG methylation and genomic footprinting at the tyrosine aminotransferase gene: DNA methylation alone is not sufficient to prevent protein binding in vivo, EMBO J., 10, 2559, 10.1002/j.1460-2075.1991.tb07796.x

Wilks, 1982, Estrogen induces a demethylation at the 5' end region of the chicken vitellogenin gene, 79, 4252