Purification, crystallization and preliminary crystallographic analysis of peroxidase from the palm tree Chamaerops excelsa

International Union of Crystallography (IUCr) - Tập 67 Số 12 - Trang 1641-1644 - 2011
Larissa C. Textor1, Jademilson C. Santos2, Nazaret Hidalgo Cuadrado3, Manuel G. Roig3, Galina G. Zhadan4, Valery L. Shnyrov4, Igor Polikarpov5
1[Instituto de Física de São Carlos, Universidade de Sáo Paulo, Sao Carlos, SP, Brazil]
2Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador São-carlense 400, 13560-970 São Carlos-SP, Brazil
3Departamento de Quı́mica Fı́sica, Facultad de Quı́mica, Universidad de Salamanca, 37008 Salamanca, Spain
4Departamento de Bioquímica y Biología Molecular Facultad de Biologia Universidad de Salamanca 37007 Salamanca, Spain
5University of São Paulo

Tóm tắt

Plant peroxidases are presently used extensively in a wide range of biotechnological applications owing to their high environmental and thermal stability. As part of efforts towards the discovery of appealing new biotechnological enzymes, the peroxidase from leaves of the palm tree Chamaerops excelsa (CEP) was extracted, purified and crystallized in its native form. An X-ray diffraction data set was collected at a synchrotron source and data analysis showed that the CEP crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 70.2, b = 100.7, c = 132.3 Å.

Từ khóa


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Thông tin xuất bản

International Union of Crystallography (IUCr)

Tập 67 Số 12

1641-1644

Thông tin tác giả