Purification and Characterization of Antioxidant Peptides of Pseudosciaena crocea Protein Hydrolysates

Springer Science and Business Media LLC - Tập 22 Số 1 - Trang 57
Ningning Zhang1, Chong Zhang2, Yuanyuan Chen1, Baodong Zheng1
1College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2Fuzhou Municipal Finance Office, Fuzhou 350002, China

Tóm tắt

Two peptides with antioxidant activity were isolated from Pseudosciaena crocea proteins. Pseudosciaena crocea muscle was hydrolyzed with neutral protease to obtain Pseudosciaena crocea protein hydrolysates (PCPH). After ultrafiltration through molecular weight cut-off membranes of 10, 5 and 3 kDa and assessment of free radical scavenging ability, the fraction (PCPH-IV) with the highest antioxidant activity was obtained. Several purification steps, i.e., ion exchange chromatography, gel filtration chromatography and reversed phase high performance liquid chromatography, were applied to further purify PCPH-IV. Two antioxidant peptides with the amino acid sequences Ser-Arg-Cys-His-Val and Pro-Glu-His-Trp were finally identified by LC-MS/MS.

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