Protocol for electrical modulation of ERK signaling dynamics in cell lines

STAR Protocols - Tập 3 - Trang 101752 - 2022
Kan Zhu1, Liang Guo1,2, Preena Patel1, John Albeck3, Quan Qing4, Wolfgang Losert5, Min Zhao1
1Department of Ophthalmology & Vision Science, Department of Dermatology, Institute for Regenerative Cures, University of California, Davis, Sacramento, CA 95817, USA
2College of Intelligent Systems Science and Engineering, Harbin Engineering University, Harbin, Heilongjiang, 150001, China
3Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA
4Department of Physics, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA
5Institute for Physical Science and Technology, Department of Physics, University of Maryland, College Park, MD 20742, USA

Tài liệu tham khảo

Guo, 2021, Electrically synchronizing and modulating the dynamics of ERK activation to regulate cell fate, iScience, 24, 103240, 10.1016/j.isci.2021.103240 Pargett, 2017, Single-cell imaging of ERK signaling using fluorescent biosensors, Methods Mol. Biol., 1636, 35, 10.1007/978-1-4939-7154-1_3 Song, 2007, Application of direct current electric fields to cells and tissues in vitro and modulation of wound electric field in vivo, Nat. Protoc., 2, 1479, 10.1038/nprot.2007.205 Sparta, 2015, Receptor level mechanisms are required for epidermal growth factor (EGF)-stimulated extracellular signal-regulated kinase (ERK) activity pulses, J. Biol. Chem., 290, 24784, 10.1074/jbc.M115.662247
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STAR Protocols

Tập 3

101752

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