Prothoracicotropic hormone — like activity in the embryonated eggs of gypsy moth, Lymantria dispar (L.)

Springer Science and Business Media LLC - Tập 161 - Trang 37-41 - 1991
Edward P. Masler1, Robert A. Bell1, Belgaum S. Thyagaraja2, Thomas J. Kelly1, Alexej B. Borkovec1
1Insect Neurobiology and Hormone Laboratory, Plant Sciences Institute, Agricultural Research Service, USDA, Beltsville, USA
2Department of Zoology, University of Maryland, College Park, USA.

Tóm tắt

Prothoracicotropic hormone (PTTH)-like activity was obtained from embryonated eggs of the gypsy moth, Lymantria dispar. Activity was detected using an in vitro prothoracic gland stimulation bioassay. Doseresponse kinetics of crude extract revealed a 4-fold activation range with a maximum activation of 35-fold. Nearly 70% of the activity was sensitive to denaturation by heat or organic solvent extraction. Heat and organic solvent-stable activity is due to a protein. Dose-response kinetics suggest the presence of a small molecular weight PTTH with pre-hatch eggs providing a rich source of the hormone.

Tài liệu tham khảo

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