Proteomics analysis for the identification of three species of Thunnus

Veterinary Research Communications - Tập 34 - Trang 153-155 - 2010
T. Pepe1, M. Ceruso1, A. Carpentieri2, I. Ventrone1, A. Amoresano2, A. Anastasio1
1Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Sezione di Ispezione, Napoli, Italy
2Dipartimento di Chimica organica e Biochimica Università degli Studi di Napoli “Federico II”, Napoli, Italy

Tóm tắt

The genus Thunnus comprises many species, some of higher quality and commercial value for their excellent organoleptic features, while others of lower quality and value. Consequently, these species are subjected to frequent fraudulent substitution. Increasing trade in fillet and minced fish makes the identification of external anatomical and morphological features of fish impossible. Proteomics was used for the identification of three Thunnus species. Muscle extracts were evaluated by both mono- and two-dimensional electrophoresis and mass spectrometric techniques. Preliminary results demonstrate that the tested species displays a high degree of polymorphism, making possible an accurate identification.

Tài liệu tham khảo

Acuña G, Ortiz-Riaño E, Vinagre J, García L, Kettlun AM, Puente J, Collados L, Valenzuela MA (2008) Application of capillary electrophoresis for the identification of Atlantic salmon and rainbow trout under raw and heat treatment. J Capill Electrophor Microchip Technol 10:93–99 Carrera M, Cañas B, Piñeiro C, Vázquez J, Gallardo JM (2007) De novo mass spectrometry sequencing and characterization of species-specific peptides from nucleoside diphosphate kinase B for the classification of commercial fish species belonging to the family Merlucciidae. J Proteome Res 6:3070–3080 Monti G, De Napoli L, Mainolfi P, Barone R, Guida M, Marino G, Amoresano A (2005) Monitoring food quality by microfluidic electrophoresis, gas chromatography, and mass spectrometry techniques: Effects of aquaculture on the sea bass (Dicentrarchus labrax). Anal Chem 77:2587–2594 Pandey A, Mann M (2000) Proteomics to study genes and genomes. Nature 405:837–846 Pepe T, Trotta M, Di Marco I, Anastasio A, Bautista JM, Cortesi ML (2007) Fish species identification in surimi-based products. J Agric Food Chem 55:3681–3685 Piñeiro C, Barros-Velázquez J, Stelo CG, Gallardo JM (1999) The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species. Z Lebensm Unters Forsch 208:342–348 Rehbein H, Kündiger R, Pineiro C, Perez-Martin RI 2000. Fish muscle parvalbumins as marker proteins for native and urea isoelectric focusing. Electrophoresis 21:1458–1463