Phân Tích Protein Học Của Mô Gan Ở Cá Dơm Cái Trưởng Thành (Danio rerio) Bị Phơi Bày Với Atrazine

Springer Science and Business Media LLC - Tập 62 - Trang 127-134 - 2011
Yuanxiang Jin1, Xiangxiang Zhang1, Dezhao Lu2, Zhengwei Fu1
1College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China
2College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China

Tóm tắt

Atrazine (ATZ), herbicide phổ biến nhất, là một chất ô nhiễm thường gặp trong các hệ sinh thái nước ngọt. Trong nghiên cứu này, phương pháp điện di gel hai chiều và khối phổ tách lớp laser hỗ trợ ma trận, kết hợp với phân tích mô bệnh lý, đã được sử dụng để phát hiện tổn thương gan ở cá dơm cái trưởng thành (Danio rerio) khi tiếp xúc với ATZ. Hơn 600 điểm protein gan đã được phát hiện trong mỗi gel bằng cách nhuộm bạc, và hầu hết các protein có kích thước từ 20 đến 70 kD và pH 4–9. Qua so sánh và phân tích, 7 protein đã được phát hiện là tăng biểu hiện hơn 2 lần, trong khi 6 điểm protein giảm biểu hiện hơn 2 lần sau khi tiếp xúc với 10 và 1000 μg/l ATZ trong vòng 14 ngày, điều này đã gây ra các tác động mô học ở gan cá dơm. Chúng tôi phát hiện rằng các protein thay đổi này liên quan đến nhiều quá trình sinh học tế bào, như phản ứng với stress oxy hóa, sự hình thành khối u, v.v. Kết quả cho thấy rằng ATZ ảnh hưởng toàn diện đến nhiều quá trình sinh học và tế bào trong cá dơm. Thông tin được trình bày trong nghiên cứu này sẽ hữu ích để hiểu rõ cơ chế của các tác động tiềm tàng do ATZ gây ra ở cá.

Từ khóa


Tài liệu tham khảo

Anderson NL, Anderson NG (1998) Proteome and proteomics: new technologies, new concepts, and new words. Electrophoresis 19:1853–1861 Bogyo M, James HH (2003) Proteomics and genomics. Curr Opin Chem Biol 7:2–4 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram qualities of proteins utilizing the principle of dye binding. Anal Biochem 72:248–254 Cañestro C, Godoy L, Gonzàlez-Duarte R, Albalat R (2003) Comparative expression analysis of Adh3 during arthropod, urochordate, cephalochordate, and vertebrate development challenges its predicted housekeeping role. Evol Dev 5:157–162 Culine S, Honore N, Closson V, Droz JP, Extra JM, Marty M et al (1994) A small GTP-binding protein is frequently overexpressed in peripheral blood mononuclear cells from patients with solid tumours. Eur J Cancer 30:670–674 Czarnecka AM, Campanella C, Zummo G, Cappello F (2006) Heat shock protein 10 and signal transduction: a “capsula eburnean” of carcinogenesis? Cell Stress Chaperones 11:287–294 Dong X, Zhu L, Wang J, Wang J, Xie H, Hou X et al (2009) Effects of atrazine on cytochrome P450 enzymes of zebrafish (Danio rerio). Chemosphere 77:404–412 Drosten M, Dhawahir A, Sum EY, Urosevic J, Lechuga CG, Esteban LM et al (2010) Genetic analysis of Ras signalling pathways in cell proliferation, migration and survival. EMBO J 29:1091–1104 Emili AQ, Cageny G (2000) Large scale functional analysis using peptide or protein arrays. Nat Biotechnol 18:393–397 Estonius M, Hoog JO, Danielsson O, Jomvall H (1994) Residues specific for class I11 alcohol dehydrogenase. Site-directed mutagenesis of the human enzyme. Biochemistry 33:15080–15085 Fan W, Yanase T, Morinaga H, Gondo S, Okabe T, Nomura M et al (2007) Atrazine induced aromatase expression is SF-1 dependent: implications for endocrine disruption in wildlife and reproductive cancers in humans. Environ Health Perspect 115:720–727 Forné I, Abián J, Cerdà J (2010) Fish proteome analysis: model organisms and non-sequenced species. Proteomics 10:858–872 Furuhashi M, Hotamisligil GS (2008) Fatty acid-binding proteins: role in metabolic diseases and potential as drug targets. Nat Rev Drug Discov 7:489–503 Ge J, Cong J, Sun Y, Li G, Zhou Z, Qian C et al (2010) Determination of endocrine disrupting chemicals in surface water and industrial wastewater from Beijing, China. Bull Environ Contam Toxicol 84:401–405 Gfrerer M, Wenzl T, Quan X, Platzer B, Lankmayr E (2002) Occurrence of triazines in surface and drinking water of Liaoning Province in Eastern China. J Biochem Biophys Methods 53:217–228 Goodsell DS (1999) The molecular perspective: the ras oncogene. Oncologist 4:263–264 Iborra FJ, Renau-Piqueras J, Portoles M, Boleda MD, Guerri C, Pares X (1992) Immunocytochemical and biochemical demonstration of formaldhyde dehydrogenase (class III alcohol dehydrogenase) in the nucleus. J Histochem Cytochem 40:1865–1878 Izaki K, Kinouchi H, Watanabe K, Owada Y, Okubo A, Itoh H et al (2001) Induction of mitochondrial heat shock protein 60 and 10 mRNAs following transient focal cerebral ischemia in the rat. Mol Brain Res 88:4–25 Jin YX, Chen YY, Jiang YH, Xu MK (2006) Proteome analysis of the silkworm (Bombyx mori. L) colleterial gland during different development stage. Arch Insect Biochem Physiol 61:42–50 Jin YX, Wang WY, Xu C, Fu ZW, Liu WP (2008) Induction of hepatic estrogen-responsive gene transcription by permethrin enantiomers in male adult zebrafish. Aquat Toxicol 88:146–152 Jin YX, Zhang XX, Lu DZ, Sun LW, Qian HF, Liu WP et al (2010a) Histopathological and proteomic analysis of hepatic tissue from adult male zebrafish exposed to 17β-estradiol. Environ Toxicol Pharmacol 29:91–95 Jin YX, Zhang XX, Shu LJ, Chen LF, Sun LW, Qian HF et al (2010b) Oxidative stress response and gene expression with atrazine exposure in adult female zebrafish (Danio rerio). Chemosphere 78:846–852 Karrow NA, McCay JA, Brown RD, Musgrove DL, Guo TL, Germolec DR et al (2005) Oral exposure to atrazine modulates cell-mediated immune function and decreases host resistance to the B16F10 tumor model in female B6C3F1 mice. Toxicology 2009:15–28 Kling P, Norman A, Andersson PL, Norrgren L, Förlin L (2008) Gender-specific proteomic responses in zebrafish liver following exposure to a selected mixture of brominated flame retardants. Ecotoxicol Environ Saf 71:319–327 Liang Y, Minami H, Sato F (2008) Isolation of herbicide-resistant 4-hydroxyphenylpyruvate dioxygenase from cultured Coptis japonica cells. Biosci Biotechnol Biochem 72:3059–3062 Liu Y, Wang JS, Wei YH, Zhang HX, Xu MQ, Dai JY (2008) Induction of time-dependent oxidative stress and related transcriptional effects of perfluorododecanoic acid in zebrafish liver. Aquat Toxicol 89:242–250 McElroy JA, Gangnon RE, Newcomb PA, Kanarek MS, Anderson HA, Brook JV et al (2007) Risk of breast cancer for women living in rural areas from adult exposure to atrazine from well water in Wisconsin. J Expo Sci Environ Epidemiol 17:207–214 Moran GR (2005) 4-Hydroxyphenylpyruvate dioxygenase. Arch Biochem Biophys 433:117–128 Nabby-Hansen S, Waterfield MD, Cramer R (2001) Proteomics-post-genomic cartography to understand gene function. Trends Pharmacol Sci 22:376–384 Oka T, Tooi O, Mitsui N, Miyahara M, Ohnishi Y, Takase M et al (2008) Effect of atrazine on metamorphosis and sexual differentiation in Xenopus laevis. Aqua Toxicol 87:215–226 Osborne N, Sherry J, Rendell JL, Currie S (2007) The role of hsp90 in 17a-ethynylestradiol-induced endocrine disruption in rainbow trout hepatocytes. Ecotoxicol Environ Saf 68:13–19 Rhee JS, Raisuddin S, Lee KW, Seo JS, Ki JS, Kim IC et al (2009) Heat shock protein (Hsp) gene responses of the intertidal copepod Tigriopus japonicus to environmental toxicants. Comp Biochem Phys C 149:104–112 Rusiecki JA, De Roos A, Lee WJ, Dosemeci M, Lubin JH, Hoppin JA et al (2004) Cancer incidence among pesticide applicators exposed to atrazine in the Agricultural Health Study. J Natl Cancer Inst 96:1375–1382 Salaberria I, Hansen BH, Asensio V, Olsvik PA, Andersen RA, Jenssen BM (2009) Effects of atrazine on hepatic metabolism and endocrine homeostasis in rainbow trout (Oncorhynchus mykiss). Toxicol Appl Pharmacol 234:98–106 Sanchez BC, Ralston-Hooper KJ, Kowalski KA, Inerowicz HD, Adamec J, Sepúlveda MS (2009) Liver proteome response of largemouth bass (Micropterus salmoides) exposed to several environmental contaminants: potential insights into biomarker development. Aquat Toxicol 95:52–59 Sanderson JT, Seinen W, Giesy JP, van den Berg M (2000) 2-Chloros-triazine herbicides induce aromatase (CYP19) activity in H295R human adrenocortical carcinoma cells: a novel mechanism for estrogenicity? Toxicol Sci 54:121–127 Sanghani PC, Robinson H, Bennett-Lovsey R, Hurley TD, Bosron WF (2003) Structure-function relationships in human class III alcohol dehydrogenase (formaldehyde dehydrogenase). Chem Biol Interact 143(44):195–200 Sass JB, Colangelo A (2006) European Union bans atrazine, while the United States negotiates continued use. Int J Occup Environ Health 12:260–267 Shaw M, Furnas MJ, Fabricius K, Haynes D, Carter S, Eaglesham G et al (2010) Monitoring pesticides in the Great Barrier Reef. Mar Pollut Bull 60:113–122 Slipicevic A, Jørgensen K, Skrede M, Katrine A, Rosnes R, Trøen G, Davidson B, Flørenes VA (2008) The fatty acid binding protein 7 (FABP7) is involved in proliferation and invasion of melanoma cells. BMC Cancer 8:276 Song Y, Zhu LS, Xie H, Wang J, Wang JH, Liu W et al (2009) Effects of atrazine on DNA damage and antioxidative enzymes in Vicia faba. Environ Toxicol Chem 28:1055–1062 Suzawa M, Ingraham HA (2008) The herbicide atrazine activates endocrine gene networks via non-steroidal NR5A nuclear receptors in fish and mammalian cells. PloS One 3:e2117 Thornton BJ, Elthon TE, Cerny RL, Siegfried BD (2010) Proteomic analysis of atrazine exposure in Drosophila melanogaster (Diptera: Drosophilidae). Chemosphere 81:235–241 United States Department of Agriculture National Agricultural Statistical Service (2008) Agricultural chemical use database. http://www.pestmanagment.info/nass/ Unwin RD, Smith DL, Blinco D, Wilson CL, Miller CJ, Evans CA et al (2006) Quantitative proteomics reveals posttranslational control as a regulatory factor in primary hematopoietic stem cells. Blood 107:4687–4694 Wennerberg K, Rossman KL, Channing J, Der CJ (2005) The Ras superfamily at a glance. J Cell Sci 118:843 (National Agricultural Statistical Service 846) Wit MD, Keil D, Remmerie N, van der Ven K, van den Brandhof EJ, Knapen D et al (2008) Molecular targets of TBBPA in zebrafish analysed through integration of genomic and proteomic approaches. Chemosphere 74:96 (National Agricultural Statistical Service 105) Yang C, Pflugrath JW, Camper DL, Foster ML, Pernich DJ, Walsh TA (2004) Structural basis for herbicidal inhibitor selectivity revealed by comparison of crystal structures of plant and mammalian 4-hydroxyphenylpyruvate dioxygenases. Biochemistry 43:10414 (National Agricultural Statistical Service 10423) Zheng Y, Quilliam LA (2003) Activation of the Ras superfamily of small GTPases. EMBO Rep 4:463–468