Protective potential of L. acidophilus in murine giardiasis

Walter de Gruyter GmbH - 2010
Gaurav Shukla1, Tarveen Kaur1, Rakesh Sehgal2, Praveen Rishi1, Vijay Prabha1
11Department of Microbiology, Panjab University, Chandigarh, 160014, India
22Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India

Tóm tắt

AbstractThis study describes the in vivo activity of Lactobacillus acidophilus in Giardia lamblia infected BALB/c mice. Experimentally, it was observed that daily administration of lactobacilli 7 days before or in simultaneous inoculation with Giardia trophozoites efficiently reduced G. lamblia infection in mice. More specifically, excretion of Giardia cysts were reduced significantly in probiotic-treated groups, and resolution of infection was observed by day 21 post-inoculation. It was also observed that the lactobacillus count increased tremendously and continuously in faeces of all probiotic-fed mice, and was significantly higher as compared with that in control mice. Histological analysis of microvilli membrane integrity revealed that probiotic administration also protected mice against parasite-induced mucosal damage, whereas Giardia-infected mice had severe villous atrophy, oedema, vacuolation and ileitis. Immunologically, the anti-Giardia serum IgG level was not stimulated significantly by probiotic treatment administered both prior to and simultaneous with Giardia infection, but remained high after the infection peak. Taken together, the data demonstrates the anti-giardial effect of the probiotic in vivo by modulation of the intestinal epithelial cells, inhibiting the colonization of Giardia trophozoites and thereby reducing the severity of Giardia infection.

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Tài liệu tham khảo

Eckmann L., Mucosal defences against Giardia, Parasite Immunology, 2003, 25, 259–270

Upcroft P., Upcroft J. A., Drug targets and mechanisms and of resistance in the anaerobic protozoa, Clinical Microbiol. Rev., 2001,14, 150–164

Eckmanm L., Gillin F.D., Microbes and microbial toxis: paradigms for microbial-mucosal interactions. Pathophysiological aspects of enteric infections with the lumen dwelling protozoan Giardia lamblia, Am. J. Physio. Gestroenterology, 2001, 280, G1–G6

Khanna R., Vinayak V.K., Mehta F.S., Kumkum R.K., Nain C.K., Giardia lamblia infection in immunosuppressed animals causes severe alterations to brush border membrane enzymes, Dig. Dis. Sci., 1998,33, 1147–1152

Walia B.N., Ganguly N.K., Mahajan R.C., Kimar D., Madan I.J., Gambhir S.K., Kanwar S.S., Morbidity in pre school giardia cyst excretors, Trop. Geograp. Med., 1986, 38(4), 367–370

Gardner T.B., Hill D.R., Treatment of Giardiasis, Clin. Microbiol. Rev., 2001,14: 114–128

Calzada F., Meckes M., Cedillo-Rivera R., Antiamoebic and antigiardial activity of plant flavonoids, Planta. Med., 1999, 65, 78–80

Aggarwal A.K., Singh M., Gupta N., Management of giardiasis by an immuno-modulatory herbal drug Pippali rasayana, J. Ethropharmacol., 1994, 44,143–146

Kaneda Y., Torii M., Tanaka T., Aikawa M., In vitro effects of berberine sulphate on the growth and structure of Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis, Ann. Trop. Med. Parasitol., 1991, 85, 417–425

Oyetayo V.O., Adetuyi F.C., Akinyosoye F.A., Safety and protective effect of Lactobacillus acidophilus and Lactobacillus casei used as probiotic agent in vivo, Afr. J. Biotechnol. 2003, 2 (11), 448–452

Aattouri N., Bouras M., Tome D., Marcos A., Lemonnier D., Oral ingestion of lactic acid bacteria by rats increases lymphocyte proliferation and interferon — production, British. J., 2001, 87:367–373

Koop-Hoolihan L., Prophylactic and therapeutic uses of probiotics: A review, J. Am. Dietetic. Assoc., 2001, File: //G:/ Intern 1

Diamond L.S., Technique of axenic culture of E. histolytica schaudinn, 1903 and E. histolytica like amoebae, J. Parasitol., 1963, 64, 1047–1056

Benyacoub J., Perez P.F., Rochat F., Saudan K.Y., Reuteler G., Antille N., Humen M., De Antoni G.L., Cavadini C., Blum S., Schiffrin E.J., Enterococcus faecium SF68 enhances the immune response to Giardia intestinalis in mice, J. of Nutrition, 2005, 135, 1171–1176

Tiwari R.P., Hoondal G.S., Tewari R., Laboratory Techniques in Microbiology and Biotechnology, 2001, Abhishek Publication

Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J., Protein measurement with folin’s phenol reagent, J. Bio. Chem. 1951, 193, 265–275

Hudson L., Hay F.C., The lymphocyte: its role and function, Practical Immunology, 1980, Third edition, Blackwell Scientific Publication, Oxford, London

Oyetayo V.O., Oyetayo F.L., Potential of probiotics as biotherapeutic agents targeting the innate immune system, Afr J Biotechnol 2005, 4 (2), 123–127

Singer S.M., Nash T.E., T-cell dependent control of acute Giardia lamblia infections in mice, Infect. Immunity, 2000, 68, 170–175

Humen M.A.L., De Antoni J., Benyacoud M.B., Costas M.I., Cardozo L., Kozubsky K., Saudan A., Doenzli-Bruand S., Blum E.J., Schiffrin P.F., Perez Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo, Infect. Immun., 2005, 73, 1265–1269

Perdigon G., Vintini E., Alvarez S., Medina M., Medici M., Study of the possible mechanisms involved in the mucosal immune system activation by lactic acid bacteria, J. Dairy Sci., 1999, 82, 1108–1114

Salminen S., Bouley C. Boutron-Ruault M.C., Functional food science and gastrointestinalphysiology and function, Br. J. Nutr., 1998, (suppl) s, 147–71

Isolauri E., Kalia M., Arvola T., Diet during rotavirus enteritis affects jejunal permeability to macromolecules in suckling rats, Pediatr. Res., 1993, 33, 548–53

Farthing M.J.G., Diarrhoeal disease: current concepts and future challenges (pathogenesis of giardiasis), Trans. R. Soc. Trop. Med. Hyg., 1993, 87, S17–S21

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