Protease from Mucor subtilissimus UCP 1262: Evaluation of several specific protease activities and purification of a fibrinolytic enzyme

Thiago Pajeú Nascimento1, Amanda Emmanuelle Sales2, José Arion da Silva Moura3, Juanize Matias da Silva Batista1, Romero Marcos Pedrosa Brandão Costa4, Camila Souza Porto5, Galba Maria de Campos‐Takaki6, Tatiana Souza Porto7, Ana Lúcia Figueiredo Porto1
1Federal Rural University of Pernambuco, Department of Morphology and Animal Physiology, Dom Manuel de Medeiros, s/n, Dois Irmaos, 52171-900 Recife, PE, Brazil
2University of South Florida, Department of Molecular Medicine, College of Medicine, Bruce B. Downs Blvd, MDC 3518, 12901, Tampa, FL, U.S.A.
3Federal University of Pernambuco, Department of Pharmaceutics Sciences, Av. Prof. Moraes Rego, 1235, Cidade Universitaria, 50670-420 Recife, PE, Brazil
4University of Pernambuco, Institute of Biological Sciences, Arnobio Marques, 310, Santo Amaro, 50100-130 Recife, PE, Brazil
5Federal University of Alagoas, Unit of Penedo, s/n, Av. Duque de Caxias, 1074, 57200-000 Penedo, AL, Brazil
6Catholic University of Pernambuco, Center for Research in Environmental Sciences, R. do Principe, 526, Boa Vista, 50050-900 Recife, PE, Brazil
7Federal Rural University of Pernambuco, Academic Unit of Garanhuns, Av. Bom Pastor, s/n, Boa Vista, 55296-901 Garanhuns, PE, Brazil

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ADETUNJI AI, 2020, Statistical modelling and optimization of protease production by an autochthonous Bacillus aryabhattai Ab15-ES: A response surface methodology approach, Biocatal Agric Biotechnol, 10.1016/j.bcab.2020.101528

AL-DHABI NA, 2020, Isolation and screening of Streptomyces sp, Saudi J Biol Sci, 27, 474

ANBU P, 2005, Purification of keratinase from poultry farm isolate Scopulariopsis brevicaulis and statistical optimization of enzyme activity, Enzyme Microb Tech, 36, 639, 10.1016/j.enzmictec.2004.07.019

ASENJO JA, 2012, Aqueous two-phase systems for protein separation: phase separation and applications, J Chromatogr A, 18, 1, 10.1016/j.chroma.2012.03.049

BARZKAR N, 2020, Marine microbial alkaline protease: Arecent developments in biofilmn ideal choice for industrial application, Int J Biol Macromol, 161, 1216, 10.1016/j.ijbiomac.2020.06.072

BRADFORD MM, 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal Biochem, 72, 248, 10.1016/0003-2697(76)90527-3

CHANG CT, 2012, Purification and biochemical properties of a fibrinolytic enzyme from Bacillus subtilis-fermented red bean, Food Chem, 133, 1611, 10.1016/j.foodchem.2012.02.061

CHAVIRA RJ, 1984, Assaying proteinases with azocoll, Anal Biochem, 136, 446, 10.1016/0003-2697(84)90242-2

CHENG-GANG C, 2008, Purification and characterization of keratinase from a new Bacillus subtilis strain, J Zhejiang University Scie B, 9, 713, 10.1631/jzus.B0820128

CHIMBEKUJWO KI, 2020, Purification, characterization and optimization conditions of protease produced by Aspergillus brasiliensis strain BCW2, Scientific African, 8, 10.1016/j.sciaf.2020.e00398

DENG Y, 2018, Dual-function chymotrypsin-like serine protease with plasminogen activation and fibrinolytic activities from the GRAS fungus,, Int J Biol Macromol, 109, 1338, 10.1016/j.ijbiomac.2017.11.142

FATIMA S, 2008, Characterization of Fluoroalcohols-Induced Intermediates of Mucor miehei Lipase at Low pH, Protein Pept Lett, 15, 346, 10.2174/092986608784246425

FRIEDRICH J, 1999, Screening fungi for synthesis of keratinolytic enzymes, Lett Appl Microbiol, 28, 127, 10.1046/j.1365-2672.1999.00485.x

GARRO MS, 2020, Solid State Fermentation in Food Processing: Advances in Reactor Design and Novel Applications, IFSET, 1, 165

GINTHER CL, 1979, Sporulation and the production of serine protease and cephamycin C by, Antimicrob Agents Chemother, 15, 522, 10.1128/AAC.15.4.522

HAMADA S, 2017, Purification and characterization of a novel extracellular neutral metalloprotease from, J Gen Appl Microbiol, 63, 51, 10.2323/jgam.2016.07.006

KIM JD, 2003, Keratinolytic activity of five Aspergillus species isolated from poultry farming soil in Korea, Mycobiol, 31, 157, 10.4489/MYCO.2003.31.3.157

KIM JS, 2008, Purification and characterization of fibrinolytic metalloprotease from Perenniporia fraxinea mycelia, Mycol Res, 112, 990, 10.1016/j.mycres.2008.01.029

KIM SH, 1998, Fibrin zymography: a direct analysis of fibrinolytic enzymes on gels, Anal Biochem, 263, 115, 10.1006/abio.1998.2816

KIM W, 1996, Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang, Appl Environ Microb, 62, 2482, 10.1128/AEM.62.7.2482-2488.1996

KUMARAN S, 2011, Purification of an intracellular fibrinolytic protease from Ganoderma lucidum Vk12 and its susceptibility to different enzyme inhibitors, Trop J Pharmaceut Res, 10, 413

LAEMMLI UK, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0

LEE JS, 2006, Purification and characterization of two novel fibrinolytic proteases from mushroom,, J Microbiol Biotechn, 16, 264

LEE SY, 2005, Purification and characterization of fibrinolytic enzyme from cultured mycelia of Armillaria mellea, Protein Expres Purif, 43, 10, 10.1016/j.pep.2005.05.004

LIU XI, 2016, Purification and characterization of a fibrinolytic enzyme from the food-grade fungus, Neurospora sitophila, J Mol Catal B Enzym, 134, 98, 10.1016/j.molcatb.2016.10.006

MAHAJAN PM, 2012, Fibrinolytic enzyme from newly isolated marine bacterium Bacillus subtilis ICTF-1: media optimization, purification and characterization, J Biosci Bioeng, 113, 307, 10.1016/j.jbiosc.2011.10.023

MASUTTI DC, 2012, Production of enzymes from rice husks and wheat straw in solid state fermentation, Chem Eng Transact, 27, 133

MEENA P, 2013, Utilization of agro-industrial waste (wheat bran) for alkaline protease production by Pseudomonas aeruginosa in SSF using Taguchi (DOE) methodology, Biocatal Agr Biotechnol, 2, 210, 10.1016/j.bcab.2013.05.003

MESHRAM V, 2016, Production, Purification and Characterisation of a Potential Fibrinolytic Protease from Endophytic Xylaria curta by Solid Substrate Fermentation, Appl Biochem Biotechnol, 181, 1496, 10.1007/s12010-016-2298-y

MOON S, 2014, Purification and characterization of a novel fibrinolytic a chymotrypsin like serine metalloprotease from the edible mushroom, Lyophyllum shimeji, J Biosci Bioeng, 117, 544, 10.1016/j.jbiosc.2013.10.019

NASCIMENTO TP, 2015, Production and characterization of new fibrinolytic protease from Mucor subtilissimus UCP 1262 in solid-state fermentation, Adv Enzyme Res, 3, 81, 10.4236/aer.2015.33009

OLUKOMAIYA OO, 2020, Solid-state fermentation of canola meal with Aspergillus sojae, Aspergillus ficuum and their co-cultures: Effects on physicochemical, microbiological and functional properties, LWT, 1

PIROTA RDPB, 2014, Enzymatic hydrolysis of sugarcane bagasse using enzyme extract and whole solid-state fermentation medium of two newly isolated strains of Aspergillus oryzae, Chem Eng Transact, 38, 259

RAVAL VH, 2014, Biochemical and structural characterization of a detergent-stable serine alkaline protease from seawater haloalkaliphilic bacteria, Process Biochem, 49, 955, 10.1016/j.procbio.2014.03.014

ROVATI JI, 2010, Novel source of fibrinolytic activity: Bionectria sp, World J Microb Biot, 26, 55

SARKAR G, 2020, Extraction and characterization of alkaline protease from Streptomyces sp, Biocataly Agric Biotechnol, 25

SHAMSI TN, 2018, Trypsin Inhibitors from Cajanus cajan and Phaseolus limensis Possess Antioxidant, Anti-Inflammatory and Antibacterial Activity, J Dietary Supplem, 15, 939, 10.1080/19390211.2017.1407383

SEMENOVA TA, 2020, Extracellular peptidases of insect-associated fungi and their possible use in biological control programs and as pathogenicity markers, Fungal Biol, 1, 65, 10.1016/j.funbio.2019.11.005

SHARKOVA TS, 2015, Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes, Microbiol, 84, 359, 10.1134/S0026261715030182

SHIRASAKA N, 2012, Purification and characterization of a fibrinolytic protease from Aspergillus oryzae KSK-3, Mycoscience, 53, 354, 10.1007/S10267-011-0179-3

SILVA GMM, 2016, Screening, production and biochemical characterization of a new fibrinolytic enzyme produced by Streptomyces sp, Acta Amazonica, 46, 323, 10.1590/1809-4392201600022

SURYIA-PRABHA M, 2015, Statistical analysis of production of protease and esterase by a newly isolated Lysinibacillus fusiformis AU01: Purification and application of protease in sub-culturing cell lines, Microbiol, 65, 33

UEDA M, 2007, Purification and characterization of fibrinolytic alkaline protease from Fusarium sp, Appl Microbiol Biot, 74, 331, 10.1007/s00253-006-0621-1

WANDERLEY MCA, 2017, Purification and characterization of a collagenase from Penicillium sp, Protein Expres Purif, 133, 8

WANG SL, 2011, Purification and biochemical characterization of a nattokinase by conversion of shrimp shell with Bacillus subtilis TKU007, New Biotechnol, 28, 196, 10.1016/j.nbt.2010.09.003

WU B, 2009, Purification and characterization of a novel fibrinolytic protease from Fusarium sp, J Ind Microbiol Biot, 36, 451, 10.1007/s10295-008-0516-5

XIAO-LAN L, 2005, Purification and characterization of a novel fibrinolytic enzyme from Rhizopus chinensis 12, Appl Microbiol Biot, 67, 209, 10.1007/s00253-004-1846-5

YAO Z, 2018, Properties of a fibrinolytic enzyme secreted by Bacillus subtilis JS2 isolated from saeu (small shrimp) jeotgal, Food Sci Biotechnol, 27, 765, 10.1007/s10068-017-0299-4

YANG H, 2019, Genome sequencing, purification, and biochemical characterization of a strongly fibrinolytic enzyme from Bacillus amyloliquefaciens Jxnuwx-1 isolated from Chinese traditional Douchi, J Gen Appl Microbiol, 66, 153, 10.2323/jgam.2019.04.005

ZHENG L, 2020, Production and characterization of a novel alkaline protease from a newly isolated Neurospora crassa through solid-state fermentation, LWT