Promotion of respiration by auxin in the induction of cell division in suspension culture
Tóm tắt
Auxins (IAA, NAA, p-CPA) caused 20–30% promotion of respiration of auxin-dependent cell cultures ofNicotiana tabacum, Glycine max, Taraxacum mongolica, andAtriplex sp. and had small if any effect on respiration of auxin-independent cell cultures ofRubus sp. andScorzonera hispanica. Antiauxin (p-CPIBA) did not affect the respiration. The auxin effect on the respiration of tobacco cells was revealed 10 min after its addition to the suspension and reached a maximum value in 60 min. This stimulation preceded the induction of cell division by auxin. Mitochondria isolated from auxin-treated tobacco cells had greater oxidative and phosphorylative activity than mitochondria from untreated cells. However, isolated mitochondria did not respond to auxin. The inhibitors of respiration (cyanide, monoiodoacetate, malonate, and 2,4-dinitrophenol) eliminated auxin effect on the respiration and cell division. It is concluded that the promotion of respiration is a common event for the auxin effects both on cell extension and on cell division. This promotion is necessary for the induction of cell division and is exerted via direct activation of mitochondriain situ.
Tài liệu tham khảo
Anderson PC, Lovrien RE, Brenner ML (1981) Energetics of the response of maize coleoptile tissue to indoleacetic acid. Planta 151:499–505
Auerswald H, Günter G (1972) Der Einfluss von MCPA auf Atmung und Enzymaktivität vonMatricaria- undChenopodium-Kallus. Wiss A Pädag Hochschule Potsdam 16:71–74
Audus LJ (1960) Effect of growth regulating substances on respiration. In: Ruhland W (ed) Encyclopedia of plant physiology XII, pt 2. Springer-Verlag, Berlin, pp 360–387
Bonner J, Bandurski RS (1952) Studies on the physiology, pharmacology and biochemistry of auxin. Annu Rev Plant Physiol 3:59–86
Gamburg KZ (1982) Regulation of cell division by auxin in isolated cultures. In: Wareing PF (ed) Plant growth substances 1982. Academic Press, New York, pp 59–67
Gamburg KZ, Osharova LM (1973) Effect of auxin on the mitotic cycle of tobacco cells grown in suspension culture (in Russian). Tsitologia 15:681–689
Key JL, Hanson JB, Bils RF (1960) Effect of 2,4-dichlorophenoxy-acetic acid application on activity and composition of mitochondria from soybean. Plant Physiol 35:177–183
Komamine AY, Shimizu T (1975) Changes in some enzymic activities and respiration in the early stage of callus formation in a carrot-root tissue culture. Physiol Plant 33:47–52
Le Tran Binh, Köhler KH, Wolf B (1977) Atmungsuntersuchungen an Geweben vonZea mays—Keimlingen im Verlauf der Kallusbildung. Biol Zbl 96:321–333
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–276
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Poljakoff-Mayber A (1955) The effect of IAA on the oxidative activity of mung bean mitochondria. J Exp Bot 6:321–326
Romani RJ, Ros TJ, Pech JC (1981) Cycloheximide stimulation of cyanide-resistant respiration in suspension cultures of senescent pear fruit cells. Plant Physiol 68:823–826
Shvetsov SG, Gamburg KZ (1981) Uptake of 2,4-dichlorophenoxyacetic acid by suspension-cultured maize cells. Biochem Physiol Pflanzen 176:676–686
Shvetsov SG, Gamburg KZ (1982) IAA uptake by isolated plant cells (in Russian). Fisiol Biochim Kult Rast (Kiev) 14:17–22
Switzer CM (1957) Effects of herbicides and related chemicals on oxidation and phosphorylation by isolated soybean mitochondria. Plant Physiol 32:42–44
Van der Plas LHW, Wagner MJ (1980) Changes in alternative oxidase activity and other mitochondrial parameters with callus formation by potato tuber tissue discs. Plant Sci Lett 17:207–213
Vojnikov VK (1979) The effects of protein synthesis inhibitors on the activity of mitochondria from winter triticale seedlings after cooling (in Russian). Fisiol Rast 26:408–414