Promoter analysis and immunolocalisation show that puroindoline genes are exclusively expressed in starchy endosperm cells of wheat grain

Plant Molecular Biology - Tập 64 - Trang 125-136 - 2007
Paul R. Wiley1,2, Paola Tosi1, Alexandre Evrard3,4, Alison Lovegrove1, Huw D. Jones1, Peter R. Shewry1
1Rothamsted Research, Hertfordshire, UK
2CSIRO Plant Industry, Canberra, Australia
3INRA, UMR 1096PIA, Montpellier Cedex 01, France
4Department of Botany and Plant Sciences, University of California, Riverside, USA

Tóm tắt

The purolindolines are small cysteine-rich proteins which are present in the grain of wheat. They have a major impact on the utilisation of the grain as they are the major determinants of grain texture, which affects both milling and baking properties. Bread and durum wheats were transformed with constructs comprising the promoter regions of the Puroindoline a (Pina) and Puroindoline b (Pinb) genes fused to the uidA (GUS) reporter gene. Nine lines showing 3:1 segregation for the transgene and comprising all transgene/species combinations were selected for detailed analysis of transgene expression during grain development. This showed that transgene expression occurred only in the starchy endosperm cells and was not observed in any other seed or vegetative tissues. The location of the puroindoline proteins in these cells was confirmed by tissue printing of developing grain, using a highly specific monoclonal antibody for detection and an antibody to the aleurone-localised 8S globulin as a control. This provides clear evidence that puroindolines are only synthesised and accumulated in the starchy endosperm cells of the wheat grain.

Tài liệu tham khảo

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