Proliferative compensation of residual radiation damage in the compartment of hematopoietic early progenitor cells of the mouse
Tóm tắt
The rate of cell entry from the compartment of hematopoietic early progenitor cells into differentiation was determined in sublethally irradiated mice. By use of the criterion of repopulating ability, transplantation of 5-(125I) iodo-2′-deoxyuridine labeled bone marrow cells into fatally irradiated syngeneic recipients allows to measure the relative number of early progenitor cells lodging in the spleen and the turnover of these cells in the donors. Following 450 rad the relative number of transplantable early progenitor cells in S-phase recovers to normal within 2 weeks and stabilizes after 5 weeks. At this time, the labeled progenitors turn over with a half-time of 1.4–2.2 days; the respective times for unirradiated mice are 1.5–1.8 days. Thus, quantitative and qualitative residual radiation damage that is known to exist in the compartment of CFU-S, is disguised within 2–5 weeks after irradiation by proliferative compensation in the entirety of early hemopoietic precursor cells which are here defined by their capacity of selfrenewal and delivery of differentiated cells and of seeding to spleens of lethally irradiated recipients.
Tài liệu tham khảo
Baum SJ, Varion MI, Vyant DE (1970) Radiation induced anemia in rats exposed repeatedly to mixed gamma-neutron radiation. Radiat Res 41:492–499
Becker AJ, McCulloch EA, Siminovitch L, Till JE (1965) The effect of different demand for blood cell production on DNA synthesis by hematopoietic colony forming cells. Blood 26:296–308
Chervenick PA, Boggs DR (1971) Patterns of proliferation and differentiation of hematopoietic stem cells after compartment depletion. Blood 37:568–580
Commerford SL, Joel DD (1979) Iododeoxyuridine administered to mice is de-iodinated and incorporated into DNA primarily as thymidylate. Biophys Res Commun 86:112–118
Cronkite EP, Carsten AL, Brecher G (1979) Hemopoietic stem cell niches, recovery from radiation and bone marrow transfusions. In: Okada S, Immamura M, Terasima T, Yamagushi H (eds) Proc. 6th Int. Congr. Radiat. Research. JARR, Tokyo, pp 648–656
Feinendegen LE, Bond VP, Cronkite EP, Hughes WL (1964) RNA turnover in normal rat bone marrow. Ann NY Acad Sci 113:727–741
Fliedner TM, Thomas ED, Meyer LM, Cronkite EP (1964) The fate of transfused H3 thymidine-labeled bone marrow cells in irradiated recipients. Ann NY Acad Sci 114:510–527
Fred SS, Smith WW (1968) Induced changes in transplantability of hemopoietic colony forming cells. Proc Soc Exp Biol Med 128:364–368
Gidali J, Lajtha LG (1971) Regulation of hemopoietic stem cell turnover in partially irradiated mice. Cell Tissue Kinet 4:31–45
Guzman E, Lajtha LG (1970) Some comparisons of the kinetic properties of femoral and splenic hemopoietic stem cells. Cell Tissue Kinet 3:91–98
Hübner G (1980) Proliferationskinetik der hämatopoietischen Stammzellen der Maus nach mehrwöchiger Rekonvaleszenz von einem Strahleninsult. Thesis. University of Köln
Hübner GE, v Wangenheim K-H, Feinendegen LE (1981) An assay for the measurement of residual damage of murine hematopoietic stem cells. Exp Hematol 9:111–117
Hughes WL, Commerford SL, Gitlin D, Krueger RC, Schultze B, Shah V, Reilly P (1965) Deoxyribonucleic acid metabolism in vivo: I. Cell proliferation and death as measured by incorporation and elimination of iododeoxyuridine. Fed Proc 23:640–648
Krueger RC, Gitlin D, Commerford SL, Stein J, Hughes WL (1960) Iododeoxyuridine (IDU) as a tracer of DNA metabolism in vivo. Fed Proc 19:307
Magli MC, Iscove NN, Odartchenco N (1982) Transient nature of early haematopoietic spleen colonies. Nature 295:527–529
Monette FC, DeMello JB (1979) The relationship between stem cell seeding efficiency and position in cell cycle. Cell Tiss Kinet 12:161–216
Part HM, Maloney MA, Lamela RA (1980) Hematopoietic stem cell proliferative behavior as revealed by bromodeoxyuridine labeling. Exp Hematol 8:1075–1079
Proukakis C, Coggle JE, Lindop PJ (1973) Some late effects of radiation in the bone marrow stem cells of the mouse. Radiol Clin Biol 42:492–499
Siegers MP, Feinendegen LE, Lahiri SK, Cronkite EP (1979) Relative number and proliferation kinetics of hemopoietic stem cells in the mouse. Blood Cells 5:211–236
Siegers MP, Feinendegen LE, Lahiri SK, Cronkite EP (1979) Proliferation kinetics of early hemopoietic precursor cells with self sustaining capacity in the mouse, studied with 125-I-labeled iodo-deoxyuridine. Exp Hematol 7:469–482
Siegers MP, v Wangenheim K-H, Hubner GE, Feinendegen LE (1981) Residual damage and discontinuity of recovery in the hematopoietic system of mice following gamma-irradiation. Exp Hematol 9:346–354
v Wangenheim K-H, Cronkite EP, Peterson HP, Hubner GE, Feinendegen LE (1982) Persisting radiation effect in the murine stem cell compartment (in preparation for Leukemia Research)
v Wangenheim K-H, Siegers MP, Feinendegen LE (1980) Repopulation ability and proliferation stimulus in the hematopoietic system of mice following gamma-irradiation. Exp Hematol 8:694–701