Programmed cell death features in apple suspension cells under low oxygen culture
Tóm tắt
Suspension-cultured apple fruit cells (Malus pumila Mill. cv. Braeburn) were exposed to a low oxygen atmosphere to test whether programmed cell death (PCD) has a role in cell dysfunction and death under hypoxic conditions. Protoplasts were prepared at various times after low oxygen conditions were established, and viability tested by triple staining with fluorescein diacetate (FDA), propidium iodide (PI) and Hoechst33342 (HO342). DNA breakdown and phosphatidylserine exposure on the plasma membrane were observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and annexin V binding. About 30% of protoplasts from cells after 48 h under low oxygen showed an increased accumulation of HO342, indicating increased membrane permeability. Positive TUNEL and annexin V results were also only obtained with protoplasts from cells under low oxygen. The results suggest that apple cell death under low oxygen is at least partially PCD mediated, and may explain tissue breakdown under controlled atmosphere (low oxygen) conditions in apple fruit.
Tài liệu tham khảo
Araya, R., Uehara, T., Nomura, Y., 1998. Hypoxia induces apoptosis in human neuroblastoma SK-N-MC cells by caspase activation accompanying cytochromec release from mitochondria.FEBS Letters,439:168–172.
Balk, J., Leaver, C.J., McCabe, P.F., 1999. Translocation of cytochromec from the mitochondria to the cytosol occurs during heat-induced programmed cell death in cucumber plants.FEBS Letters,463:151–154.
De Jong, A.J., Hoeberichts, F.A., Yakimova, E.T., Maximova, E., Woltering, E.J., 2000. Chemical-induced apoptotic cell death in tomato cells: involvement of caspase-like proteases.Planta,211:656–662.
Drew, M.C., He, C.J., Morgan, P.W., 2000. Programmed cell death and aerenchyma formation in roots.Trends in Plant Science,5:123–127.
Frearson, E.M., Power, J.B., Cocking, E.C., 1973. The isolation, culture and regeneration ofPetunia leaf protoplasts.Developmental Biology,33:130–137.
Gavrieli, Y., Sherman, Y., Ben-Sasson, S.A., 1992. Identification of programmed cell deathin situ via specific labeling of nuclear DNA fragmentation.Journal of Cell Biology,119:493–501.
Gunawardena, A.H.A.L.N., Pearce, D.M., Jackson, M.B., Hawes, C.R., Evans, D.E., 2001. Characterisation of programmed cell death during aerenchyma formation induced by ethylene or hypoxia in roots of maize (Zea mays L.).Planta,212:205–214.
Jacobson, M.D., Raff, M.C., 1995. Programmed cell death and Bcl-2 protection in very low oxygen.Nature,374: 814–816.
Jones, A., 2000. Does the plant mitochondrion integrate cellular stress and regulate programmed cell death?Trends in Plant Science,5:225–230.
Koukaková, B., Kovarík, A., Fajkus, J., Siroký, J., 1997. Chromatin fragmentation associated with apoptotic changes in tobacco cells exposed to cold stress.FEBS Letters,414:289–292.
Langebartels, C., Wohlgemuth, H., Kschieschan, S., Grun, S., Sandermann, H., 2002. Oxidative burst and cell death in ozone-exposed plants.Plant Physiology and Biochemistry,40:567–575.
Lau, O.L., 1998. Effect of growing season, harvest maturity, waxing, low O2 and elevated CO2 on flesh browning disorders in ‘Braeburn’ apples.Postharvest Biology and Technology,14:131–141.
McCabe, P.F., Leaver, C.L., 2000. Programmed cell death in cell cultures.Plant Molecular Biology,44:359–368.
McCabe, P.F., Levine, A., Meijer, P.J., Tapon, N.A., Pennell, R.I., 1997. A programmed cell death pathway activated in carrot cells cultured at low cell density.Plant Journal,12:267–280.
O’Brien, I.E.W., Reutelingsperger, C.P.M., Holdaway, K.M., 1997. Annexin-V and TUNEL use in monitoring the progression of apoptosis in plants.Cytometry,29:28–33.
O’Brien, I.E.W., Baguley, B.C., Murray, B.G., Morris, B.A.M., Ferguson, I.B., 1998. Early stages of the apoptotic pathway in plant cells are reversible.Plant Journal,13:803–814.
Pasqualini, S., Piccioni, C., Reale, L., Ederli, L., Torre, G.D., Ferranti, F., 2003. Ozone-induced cell death in tobacco cultivar Bel W3 plants. The role of programmed cell death in lesion formation.Plant Physiology,133: 1122–1134.
Pennell R.I., Lamb, C., 1997. Programmed cell death in plants.Plant Cell,9:1157–1168.
Schmid, I., Uittenbogaart, C.H., Giorgi, J.V., 1994. Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry.Cytometry,15:12–20.
Wang, C.Y., Bowen, J.H., Weir, I.E., Allan, A.C., Ferguson, I.B., 2001. Heat-induced protection against death of suspension-cultured apple fruit cells exposed to low temperature.Plant, Cell and Environment,24:1199–1207.
Woltering, E.J., van der Bent, A., Hoeberichts, F.A., 2002. Do plant caspases exist?Plant Physiology,130:1764–1769.
Xu, C.J., Chen, K.S., Ding, H., Weir, I.E., Ferguson, I.B., 2004. A review of methods for measuring plant programmed cell death (PCD).Hereditas, in press (in Chinese with English abstract).