Profiling microRNA expression in fracture nonunions

Bone and Joint Journal - Tập 97-B Số 8 - Trang 1144-1151 - 2015
Takahiro Waki1, S. Y. Lee2, Takahiro Niikura2, Takashi Iwakura1, Yoshihiro Dogaki1, Etsuko Okumachi1, Ryosuke Kuroda3, Masahiro Kurosaka4
1Orthopaedic Surgeon, Department of Orthopaedic SurgeryKobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
2Assistant Professor, Department of Orthopaedic SurgeryKobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
3Associate Professor, Department of Orthopaedic SurgeryKobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
4Professor and Chairman, Department of Orthopaedic SurgeryKobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.

Tóm tắt

MicroRNAs (miRNAs ) are small non-coding RNAs that regulate gene expression. We hypothesised that the functions of certain miRNAs and changes to their patterns of expression may be crucial in the pathogenesis of nonunion. Healing fractures and atrophic nonunions produced by periosteal cauterisation were created in the femora of 94 rats, with 1:1 group allocation. At post-fracture days three, seven, ten, 14, 21 and 28, miRNAs were extracted from the newly generated tissue at the fracture site. Microarray and real-time polymerase chain reaction (PCR) analyses of day 14 samples revealed that five miRNAs, miR-31a-3p, miR-31a-5p, miR-146a-5p, miR-146b-5p and miR-223-3p, were highly upregulated in nonunion. Real-time PCR analysis further revealed that, in nonunion, the expression levels of all five of these miRNAs peaked on day 14 and declined thereafter. Our results suggest that miR-31a-3p, miR-31a-5p, miR-146a-5p, miR-146b-5p and miR-223-3p may play an important role in the development of nonunion. These findings add to the understanding of the molecular mechanism for nonunion formation and may lead to the development of novel therapeutic strategies for its treatment. Cite this article: Bone Joint J 2015; 97-B:1144–51.

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Tài liệu tham khảo

10.1302/0301-620X.89B12.19671

10.1302/0301-620X.88B7.17639

10.1002/jor.20182

10.2106/00004623-200307000-00010

10.1016/S0092-8674(04)00045-5

10.1038/nature03702

10.1007/s11914-013-0143-6

10.1038/nm.3026

10.1002/jbmr.2175

10.1002/jor.1100020115

10.1016/S0736-0266(02)00209-7

10.1006/meth.2001.1262

10.1097/00003086-199810001-00003

10.1038/nrrheum.2012.1

10.1359/jbmr.2003.18.9.1584

10.1159/000047893

10.1016/j.ccr.2011.12.015

10.1038/33429

10.1073/pnas.0605298103

10.1111/joim.12099

10.1371/journal.pone.0042971

10.1016/j.bbrc.2014.02.058

10.1002/jcb.23106

10.1016/j.gene.2013.06.021

10.1371/journal.pone.0038648

David NB, 2002, Development, 129, 4457, 10.1242/dev.129.19.4457

10.1038/ni1251

10.1002/art.24330

10.1038/ncb1741

10.1093/nar/gkt666

10.1038/sj.mt.6300311

Janssen HL, 2013, N Engl J Med, 369, 878

10.1016/S0736-0266(01)00142-5

10.1302/2046-3758.26.2000165

10.1302/0301-620X.94B7.27370

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Thông tin xuất bản

Bone and Joint Journal

Tập 97-B Số 8

1144-1151

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