Production of membrane proteins for NMR studies using the condensed single protein (cSPP) production system
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Aramini JM, Rossi P, Anklin C, Xiao R, Montelione GT (2007) Microgram-scale protein structure determination by NMR. Nat Methods 4:491–493
Arora A, Abildgaard F, Bushweller JH, Tamm LK (2001) Structure of outer membrane protein A transmembrane domain by NMR spectroscopy. Nat Struct Biol 8:334–338
Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277–293
Fernandez C, Adeishvili K, Wuthrich K (2001) Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles. Proc Natl Acad Sci U S A 98:2358–2363
Goddard TD, Kneller DG SPARKY 3. University of California, San Francisco
Krueger-Koplin RD, Sorgen PL, Krueger-Koplin ST, Rivera-Torres IO, Cahill SM, Hicks DB, Grinius L, Krulwich TA, Girvin ME (2004) An evaluation of detergents for NMR structural studies of membrane proteins. J Biomol NMR 28:43–57
Pervushin K, Riek R, Wider G, Wuthrich K (1997) Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. Proc Natl Acad Sci U S A 94:12366–12371
Qing G, Ma LC, Khorchid A, Swapna GV, Mal TK, Takayama MM, Xia B, Phadtare S, Ke H, Acton T et al (2004) Cold-shock induced high-yield protein production in Escherichia coli. Nat Biotechnol 22:877–882
Schneider WM, Tang Y, Vaiphei ST, Mao L, Shen Y, Raman S, Baker D, Inouye M, Roth MJ, and Montelione GT (2009). Efficient production of perdeuterated soluble and membrane proteins for NMR studies. Nat Methods (Submitted)
Sorgen PL, Hu Y, Guan L, Kaback HR, Girvin ME (2002) An approach to membrane protein structure without crystals. Proc Natl Acad Sci U S A 99:14037–14040
Suzuki M, Mao L, Inouye M (2007) Single protein production (SPP) system in Escherichia coli. Nat Protoc 2:1802–1810
Suzuki M, Roy R, Zheng H, Woychik N, Inouye M (2006) Bacterial bioreactors for high yield production of recombinant protein. J Biol Chem 281:37559–37565
Suzuki M, Zhang J, Liu M, Woychik NA, Inouye M (2005) Single protein production in living cells facilitated by an mRNA interferase. Mol Cell 18:253–261
