Production of lipids in 10 strains of Chlorella and Parachlorella, and enhanced lipid productivity in Chlorella vulgaris

Springer Science and Business Media LLC - Tập 94 Số 2 - Trang 549-561 - 2012
Pavel Přibyl1, Vladislav Cepák1, Vilém Zachleder2
1Algological Centre and Centre for Bioindication and Revitalization, Institute of Botany, v.v.i., Academy of Sciences of the Czech Republic, Třeboň, Czech Republic
2Laboratory of the Cell Cycles of Algae, Institute of Microbiology, Academy of Sciences of the Czech Republic, Třeboň, Czech Republic

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Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

Chen W, Sommerfeld M, Hu Q (2011) Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae. Bioresour Technol 102:135–141. doi: 10.1016/j.biortech.2010.06.076

Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306. doi: 10.1016/j.biotechadv.2007.02.001

Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26:126–131. doi: 10.1016/j.tibtech.2007.12.002

Doucha J, Lívanský K (1995) Novel outdoor thin-layer high density microalgal culture system: productivity and operation parameters. Arch Hydrobiol/Algolog Stud 76:129–147

Doucha J, Lívanský K (2006) Productivity, CO2/O2 exchange and hydraulics in outdoor open high density microalgal (Chlorella sp.) photobioreactors operated in a Middle and Southern European climate. J Appl Phycol 18:811–826. doi: 10.1007/s10811-006-9100-4

Doucha J, Lívanský K (2009) Outdoor open thin-layer microalgal photobioreactor: potential productivity. J Appl Phycol 21:111–117. doi: 10.1007/s10811-008-9336-2

Doucha J, Straka F, Lívanský K (2005) Utilization of flue gas for cultivation of microalgae (Chlorella sp.) in an outdoor open thin-layer photobioreactor. J Appl Phycol 17:403–412. doi: 10.1007/s10811-005-8701-7

Doušková I, Doucha J, Lívanský K, Machát J, Novák P, Umysová D, Zachleder V, Vítová M (2009) Simultaneous flue gas bioremediation and reduction of microalgal biomass production costs. Appl Microbiol Biotechnol 82:179–185. doi: 10.1007/s00253-008-1811-9

Eltgroth ML, Watwood RL, Wolfe GV (2005) Production and cellular localization of neutral long-chain lipids in the haptophyte algae Isochrysis galbana and Emiliania huxleyi. J Phycol 41:1000–1009. doi: 10.1111/j.1529-8817.2005.00128.x

Feng YJ, Li C, Zhang DW (2011) Lipid production of Chlorella vulgaris cultured in artificial wastewater medium. Bioresour Technol 102:101–105. doi: 10.1016/j.biortech.2010.06.016

Henderson RJ, Sargent JR (1989) Lipid composition and biosynthesis in aging cultures of the marine cryptomonad Chroomonas salina. Phytochemistry 28:1355–1362. doi: 10.1016/S0031-9422(00)97745-8

Heredia-Arroyo T, Wei W, Hu B (2010) Oil accumulation via heterotrophic/mixotrophic Chlorella protothecoides. Appl Biochem Biotechnol 162:1978–1995. doi: 10.1007/s12010-010-8974-4

Ho SH, Chen WM, Chang JS (2010) Scenedesmus obliquus CNW-N as a potential candidate for CO2 mitigation and biodiesel production. Bioresour Technol 101:8725–8730. doi: 10.1016/j.biortech.2010.06.112

Illman AM, Scragg AH, Shales SW (2000) Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzym Microb Technol 27:631–635. doi: 10.1016/S0141-0229(00)00266-0

Khozin-Goldberg I, Cohen Z (2006) The effect of phosphate starvation on the lipid and fatty acid composition of the fresh water eustigmatophyte Monodus subterraneus. Phytochemistry 67:696–701. doi: 10.1016/j.phytochem.2006.01.010

Kosaric N, Velikonja J (1995) Liquid and gaseous fuels from biotechnology: challenge and opportunities. FEMS Microbiol Rev 16:111–142. doi: 10.1111/j.1574-6976.1995.tb00161.x

Kvíderová J, Lukavský J (2005) The ecological characteristics of Stichococcus (Chlorophyta) strains isolated from polar and temperate regions. Arch Hydrobiol/Algolog Stud 118:127–140

Li XF, Xu H, Wu QY (2007) Large-scale biodiesel production from microalga Chlorella protothecoides through heterotrophic cultivation in bioreactors. Biotechnol Bioeng 98:764–771. doi: 10.1002/bit.21489

Li PL, Miao XL, Li RX, Zhong JJ (2011) In situ biodiesel production from fast-growing and high oil content Chlorella pyrenoidosa in rice straw hydrolysate. J Biomed Biotechnol. doi: 10.1155/2011/141207

Liu ZY, Wang GC, Zhou BC (2008) Effect of iron on growth and lipid accumulation in Chlorella vulgaris. Bioresour Technol 99:4717–4722. doi: 10.1016/j.biortech.2007.09.073

Liu J, Huang JC, Fan KW, Jiang Y, Zhong YJ, Sun Z, Chen F (2010) Production potential of Chlorella zofingienesis as a feedstock for biodiesel. Bioresour Technol 101:8658–8663. doi: 10.1016/j.biortech.2010.05.082

Liu J, Huang J, Sun Z, Zhong Y, Jiang Y, Chen F (2011) Differential lipid and fatty acid profiles of photoautotrophic and heterotrophic Chlorella zofingiensis: assessment of algal oils for biodiesel production. Bioresour Technol 102:106–110. doi: 10.1016/j.biortech.2010.06.017

Lukavský J (1982) Cultivation of chlorococcal algae in crossed gradients of temperature and light. Arch Hydrobiol/Algolog Stud 29:517–528

Meier RL (1955) Biological cycles in the transformation of solar energy into useful fuels. In: Daniels F, Duffie JA (eds) Solar energy research. University of Wisconsin Press, Madison, pp 179–184

Milner HW (1948) The fatty acids of Chlorella. J Biol Chem 176:813–817

Mock T, Kroon BMA (2002) Photosynthetic energy conversion under extreme conditions—I: important role of lipids as structural modulators and energy sink under N-limited growth in Antarctic sea ice diatoms. Phytochemistry 61:41–51. doi: 10.1016/S0031-9422(02)00216-9

Pal D, Khozin-Goldberg I, Cohen Z, Boussiba S (2011) The effect of light, salinity, and nitrogen availability on lipid production by Nannochloropsis sp. Appl Microbiol Biotechnol 90:1429–1441. doi: 10.1007/s00253-011-3170-1

Park JBK, Craggs RJ, Shilton AN (2011) Wastewater treatment high rate algal ponds for biofuel production. Bioresour Technol 102:35–42. doi: 10.1016/j.biortech.2010.06.158

Piorreck M, Baasch KH, Pohl P (1984) Biomass production, total protein, chlorophyll, lipids and fatty acids of freshwater green and blue algae under different nitrogen regimes. Phytochemistry 23:207–216. doi: 10.1016/S0031-9422(00)80304-0

Pittman JK, Dean AP, Osundeko O (2011) The potential of sustainable algal biofuel production using wastewater resources. Bioresour Technol 102:17–25. doi: 10.1016/j.biortech.2010.06.035

Přibyl P, Eliáš M, Cepák V, Lukavský J, Kaštánek P (2012) Zoosporogenesis, morphology, ultrastructure, pigment composition and phylogenetic position of Trachydiscus minutus (Eustigmatophyceae, Heterokontophyta). J Phycol 48:231–242 doi: 10.1111/j.1529-8817.2011.01109.x

Ramazanov A, Ramazanov Z (2006) Isolation and characterization of a starchless mutant of Chlorella pyrenoidosa STL-PI with a high growth rate, and high protein and polyunsaturated fatty acid content. Phycol Res 54:255–259. doi: 10.1111/j.1440-1835.2006.00416.x

Reitan KI, Rainuzzo JR, Olsen Y (1994) Effect of nutrient limitation on fatty acid and lipid content of marine microalgae. J Phycol 30:972–979. doi: 10.1111/j.0022-3646.1994.00972.x

Richardson B, Orcutt DM, Schwertner HA, Martinez CL, Wickline HE (1969) Effects of nitrogen limitation on the growth and composition of unicellular algae in continuous culture. Appl Microbiol 18:245–250

Rodolfi L, Chini Zitelli G, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR (2009) Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102:100–112. doi: 10.1002/bit.22033

Roessler PG (1990) Environmental control of glycerolipid metabolism in microalgae: commercial implications and future research directions. J Phycol 26:393–399. doi: 10.1111/j.0022-3646.1990.00393.x

Sheehan J, Dunahay T, Benemann J, Roessler P (1998) A look back at the U.S. Department of Energy’s Aquatic Species Program—biodiesel from algae, close out report TP-580-24190. National Renewable Energy Laboratory, Golden

Shen Y, Yuan W, Pei Z, Mao E (2010) Heterotrophic culture of Chlorella protothecoides in various nitrogen sources for lipid production. Appl Biochem Biotechnol 160:1674–1684. doi: 10.1007/s12010-009-8659-z

Shifrin NS, Chisholm SW (1981) Phytoplankton lipids: interspecific differences and effects of nitrate, silicate and light-dark cycles. J Phycol 17:374–384. doi: 10.1111/j.0022-3646.1981.00374.x

Sirisansaneeyakul S, Singhasuwan S, Choorit W, Phoopat N, Garcia JL, Chisti Y (2011) Photoautotrophic production of lipids by some Chlorella strains. Mar Biotechnol 13:928–941. doi: 10.1007/s10126-010-9355-2

Spoehr HA, Milner HW (1949) The chemical composition of Chlorella; effect of environmental conditions. Plant Physiol 24:120–149. doi: 10.1104/pp.24.1.120

Stauffer E (2005) A review of the analysis of vegetable oil residues from fire debris samples: spontaneous ignition, vegetable oils, and the forensic approach. J Forensic Sci 50:1–10. doi: 10.1111/j.1556-4029.2006.00220.x

Tang HY, Chen M, Garcia MED, Abunasser N, Ng KYS, Salley SO (2011) Culture of microalgae Chlorella minutissima for biodiesel feedstock production. Biotechnol Bioeng 108:2280–2287. doi: 10.1002/bit.23160

Wan M, Liu P, Xia J, Rosenberg JN, Oyler GA, Betenbaugh MJ, Nie Z, Qiu G (2011) The effect of mixotrophy on microalgal growth, lipid content, and expression levels of three pathway genes in Chlorella sorokiniana. Appl Microbiol Biotechnol 91:835–844. doi: 10.1007/s00253-011-3399-8

Wood BJB (1988) Lipids of algae and protozoa. In: Ratledge C, Wilkinson SG (eds) Microbial lipids, vol 1. Academic, London, pp 807–867

Wright L (2006) Worldwide commercial development of bioenergy with a focus on energy crop-based projects. Biomass Bioenerg 30:706–714. doi: 10.1016/j.biombioe.2005.08.008

Xiong W, Li XF, Xiang JY, Wu QY (2008) High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production. Appl Microbiol Biotechnol 78:29–36. doi: 10.1007/s00253-007-1285-1

Zachleder V, Šetlík I (1982) Effect of irradiance on the course of RNA synthesis in the cell cycle of Scenedesmus quadricauda. Biol Plantarum 24:341–353. doi: 10.1007/BF02909100

Zheng HL, Yin JL, Gao Z, Huang H, Ji XJ, Dou C (2011) Disruption of Chlorella vulgaris cells for the release of biodiesel-producing lipids: a comparison of grinding, ultrasonication, bead milling, enzymatic lysis, and microwaves. Appl Biochem Biotechnol 164:1215–1224. doi: 10.1007/s12010-011-9207-1