Production of indole alkaloids by selected hairy root lines of Catharanthus roseus

Biotechnology and Bioengineering - Tập 41 Số 5 - Trang 581-592 - 1993
Rajiv Bhadra1, Sundeep Vani1, Jacqueline V. Shanks1
1Department of Chemical Engineering, Institute of the Biosciences and Bioengineering, Rice University, Houston, TX 77251

Tóm tắt

Abstract

Hairy root cultures of Catharanthus roseus were established by infection of seedlings with Agrobacterium rhizogenes 15834. Approximately 150 transformants from four different. C. roseus cultivars were screened for desirable traits in growth and indole alkaloid production. Five hairy root clones grew well in liquid culture with doubling times similar to those reported for cell suspensions. Fast growing clones had similar morphologies, characterized by thin, straight, and regular branches with thin tips. The levels of key alkaloids, ajmalicine, serpentine, and catharanthine, in these five clones, also compared well with literature data from cell suspensions, yet HPLC and GC‐MS data indicate the presence of vindoline in two clones at levels over three orders of magnitude greater than the minute amounts reported in cell culture. These results suggest that further optimization may result in hairy roots as a potential source of vindoline and catharanthine, the two monomers necessary to synthesize that antineoplastic drug, vinblastine. © 1993 John Wiley & Sons, Inc.

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Biotechnology and Bioengineering

Tập 41 Số 5

581-592

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