Process analysis of reduced specific productivity of TNFR-Fc in Chinese hamster ovary cells at high cell density

Mohler, 1993, Soluble tumor necrosis factor (TNF) receptors are effective therapeutic agents in lethal endotoxemia and function simultaneously as both TNF carriers and TNF antagonists, J Immunol, 151, 1548, 10.4049/jimmunol.151.3.1548 Ducharme, 2008, Etanercept, Expert Opin Biol Ther, 8, 491, 10.1517/14712598.8.4.491 Hwang, 2009, Effect of Akt overexpression on programmed cell death in antibody-producing Chinese hamster ovary cells, J Biotechnol, 139, 89, 10.1016/j.jbiotec.2008.09.008 Bibila, 1995, In pursuit of the optimal fed-batch process for monoclonal antibody production, Biotechnol Prog, 11, 1, 10.1021/bp00031a001 Borth, 2005, Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line, Biotechnol Prog, 21, 106, 10.1021/bp0498241 Munzert, 1996, Sialidase activity in culture fluid of Chinese hamster ovary cells during batch culture and its effect on recombinant human antithrombin integrity, Biotechnol Prog, 12, 559, 10.1021/bp9600086 Wlaschin, 2006, Fedbatch culture and dynamic nutrient feeding, Adv Biochem Eng Biotechnol, 101, 43 Kantardjieff, 2010, Transcriptome and proteome analysis of Chinese hamster ovary cells under low temperature and butyrate treatment, J Biotechnol, 145, 143, 10.1016/j.jbiotec.2009.09.008 Rodriguez, 2005, Enhanced production of monomeric interferon-β by CHO cells through the control of culture conditions, Biotechnol Prog, 21, 22, 10.1021/bp049807b Zhao, 2009, Responses of GS-NSO myeloma cells to osmolality: cell growth, intracellular mass metabolism, energy metabolism, and antibody production, Biotechnol Bioeng Eng, 14, 625, 10.1007/s12257-008-0223-0 Sun, 2004, Hyperosmotic stress in murine hybridoma cells: effects on antibody transcription, translation, posttranslational processing, and the cell cycle, Biotechnol Prog, 20, 576, 10.1021/bp0342203 Yoon, 2003, Effect of low culture temperature on specific productivity and transcription level of anti-4-1BB antibody in recombinant Chinese hamster ovary cells, Biotechnol Prog, 19, 1383, 10.1021/bp034051m Fan, 2010, Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase Chinese hamster ovary cells, Biotechnol Lett, 32, 1239, 10.1007/s10529-010-0318-5 Zeng, 1996, Quantitative assessment of cell density effect on the metabolism and antibody production rate of hybridoma cells at high cell density, J Biotechnol, 45, 243, 10.1016/0168-1656(95)00172-7 Taticek, 1997, Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system, Biotechnol Bioeng, 54, 142, 10.1002/(SICI)1097-0290(19970420)54:2<142::AID-BIT6>3.0.CO;2-L Doverskog, 1997, Physiology of cultured animal cells, J Biotechnol, 59, 408, 10.1016/S0168-1656(97)00172-7 Pendse, 1992, Effect of cloned gene dosage on cell growth and hepatitis B surface antigen synthesis and secretion in recombinant CHO cells, Biotechnol Bioeng, 40, 119, 10.1002/bit.260400117 Zhou, 1997, Alteration of mammalian cell metabolism by dynamic nutrient feeding, Cytotechnology, 24, 99, 10.1023/A:1007945826228 Renard, 1988, Evidence that monoclonal antibody production kinetics is related to the integral of viable cells in batch systems, Biotechnol Lett, 10, 91, 10.1007/BF01024632 Chomczynski, 1987, Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction, Anal Biochem, 162, 156, 10.1016/0003-2697(87)90021-2 Goodman, 1990 Khoo, 2009, Detailed understanding of enhanced specific antibody productivity in NSO myeloma cells, Biotechnol Bioeng, 102, 188, 10.1002/bit.22041 deZengotita, 2002, Characterization of hybridoma cell responses to elevated pCO2 and osmolality: intracellular pH, cell size, apoptosis, and metabolism, Biotechnol Bioeng, 77, 369, 10.1002/bit.10176 Bibila, 1991, A model of interorganelle monoclonal antibody transport and secretion in mouse hybridoma cells, Biotechnol Bioeng, 38, 767, 10.1002/bit.260380711 Bibila, 1992, Use of a structured kinetic model of antibody synthesis and secrection for optimization of antibody productions systems: i. Steady-state analysis, Biotechnol Bioeng, 39, 251, 10.1002/bit.260390302 Yu M, Hu Z, Pacis E, Vijayasankaran N, Shen A, Li F. Understanding the intracellular effect of enhanced nutrient feeding toward high titer antibody production process. Biotechnol Bioeng. doi:10.1002/bit.23031. Mazur, 1998, Higher productivity of growth-arrested Chinese hamster ovary cells expressing the cyclin-dependent kinase inhibitor p27, Biotechnol Prog, 14, 705, 10.1021/bp980062h Mazur, 1999, A novel autoregulated proliferation-controlled production process using recombinant CHO cells, Biotechnol Bioeng, 65, 144, 10.1002/(SICI)1097-0290(19991020)65:2<144::AID-BIT3>3.0.CO;2-Q Bibila, 1991, A structured model for monoclonal antibody synthesis in exponentially growing and stationary phase hybridoma cells, Biotechnol Bioeng, 37, 210, 10.1002/bit.260370304 Lee, 2001, Effect of hypoosmotic pressure on cell growth and antibody production in recombinant Chinese hamster ovary cell culture, Cytotechnology, 36, 61, 10.1023/A:1014032701800 Wang, 2007, Gene transcription acceleration: main cause of Hepatitis B surface antigen production improvement by dimethyl sulfoxide in the culture of Chinese hamster ovary cells, Biotechnol Bioeng, 97, 526, 10.1002/bit.21262 Ryu, 2001, Effects of clone gene dosage on the response of recombinant CHO cells to hyperosmotic pressure in regard to cell growth and antibody production, Biotechnol Prog, 17, 993, 10.1021/bp010116e Altman, 1971 Taticek, 1998, Effect of dissolved carbon dioxide bicarbonate on mammalian cell metabolism recombinant protein productivity in high density perfusion culture Mostafa, 2003, Strategies for improved dCO2 removal in lager-scale fed-batch cultures, Biotechnol Prog, 19, 45, 10.1021/bp0256263 Zhu, 2005, Effects of elevated pCO2 and osmolality on growth of CHO cells and production of antibody-fusion protein B1: a case study, Biotechnol Prog, 21, 70, 10.1021/bp049815s Thomas, 1995, Bicarbonate briefly CO2-free, Nature, 374, 597, 10.1038/374597a0 deZengotita, 1998, Effects of CO2 and osmolality on hybridoma cells: growth, metabolism and monoclonal antibody production, Cytotechnology, 28, 213, 10.1023/A:1008010605287 Goudar, 2007, Decreased pCO2 accumulation by eliminating bicarbonate addition to high cell-density cultures, Biotechnol Bioeng, 96, 1107, 10.1002/bit.21116