Các quá trình pro-oxidant và antioxidant trong tuyến khí và các mô khác của cá tuyết (Gadus morhua)

Springer Science and Business Media LLC - Tập 163 - Trang 477-486 - 1993
P. Lemaire1, A. Viarengo2, L. Canesi2, D. R. Livingstone1
1NERC Plymouth Marine Laboratory, Plymouth, UK
2Istituto di Fisiologia Generale, University of Genoa, Genoa, Italy

Tóm tắt

Sự khử một phần phân tử oxy sinh ra các gốc oxyradicals phản ứng, bao gồm các gốc anion superoxide (O - 2 ) và gốc hydroxyl (·OH). Tuyến khí hoạt động dưới các điều kiện hyperoxic và tính axit, do đó có khả năng chịu đựng căng thẳng oxy hóa tăng cường. Các khía cạnh của quá trình pro- và antioxidant trong tuyến khí đã được so sánh với các mô khác có khả năng phải chịu các mức độ sản xuất oxyradical khác nhau, chẳng hạn như gan (nơi sản xuất oxyradical qua môi trường hóa học), mang và cơ vân. Hoạt động của các enzyme antioxidant (superoxide dismutase, catalase, glutathione peroxidase phụ thuộc vào selen và tổng số) tính trên mỗi gam trọng lượng ẩm là cao nhất ở gan và thấp nhất ở cơ. Hoạt động của catalase và glutathione peroxidase tính trên mỗi gam trọng lượng ẩm thì cao hơn ở mang so với tuyến khí, trong khi điều ngược lại được quan sát đối với superoxide dismutase. Hoạt động của superoxide dismutase cytosolic tính trên mỗi mg protein cao gấp hai và chín lần ở tuyến khí so với gan và mang. Các đặc tính pH của các enzyme antioxidant thường tương tự nhau ở tất cả các mô. Nồng độ glutathione, vitamin E và lipid không bão hòa (có khả năng bị peroxid hóa) thường cao nhất ở gan, tiếp theo là tuyến khí. Sự peroxid hóa lipid (tương đương malonaldehyde) được phát hiện ở tất cả các mô ngoại trừ tuyến khí. Hydrogen peroxide và O - 2 có liên quan đến quá trình hình thành ·OH qua trung gian ferric/EDTA phụ thuộc vào NAD(P)H (như được đo bằng sự oxy hóa 2-keto-4-methiolbutyrate) bởi các phân đoạn tiền mitochondrial và sau mitochondrial của tuyến khí. Tiềm năng tối đa của mô cho sự sản xuất ·OH tương ứng với hoạt động của superoxide dismutase nhưng không phải với hoạt động của catalase hay glutathione peroxidase. Tổng thể, các kết quả xác nhận sự hiện diện của các hàng rào phòng thủ antioxidant hiệu quả trong tuyến khí và hỗ trợ những khẳng định trước đó về vai trò trung tâm của superoxide dismutase trong quá trình này.

Từ khóa

#tuyến khí #gốc oxyradicals #superoxide dismutase #antioxidant #cá tuyết #Gan #peroxid hóa lipid

Tài liệu tham khảo

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