Prevalence of Bartonella species, Rickettsia felis, haemoplasmas and the Ehrlichia group in the blood of cats and fleas in eastern Australia

Australian Veterinary Journal - Tập 88 Số 5 - Trang 160-165 - 2010
Vanessa R. Barrs1,2, Julia A. Beatty1,2, Bethany Wilson1,2, Nicholas R. Evans3, Richard A Gowan4, Randolph M. Baral5, Amy E Lingard1,2, G Perkovic1,2, Jennifer Hawley6, Michael R. Lappin6
1Valentine Charlton Cat Centre, Faculty of Veterinary Science, The University of Sydney, New South Wales 2006, Australia
2[email protected]
3The Cat Clinic Mount Gravatt, Mt Gravatt, QLD, Australia
4The Cat Clinic, Prahran, VIC, Australia
5Paddington Cat Hospital, Paddington, NSW, Australia
6Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA

Tóm tắt

Objectives  To define the prevalence of Bartonella spp., Rickettsia felis, Mycoplasma haemofelis, ‘Candidatus Mycoplasma haemominutum’ (Mhm) and ‘Candidatus Mycoplasma turicensis’ (Mtc) in cats and their fleas in eastern Australia.

Design and procedure  Conventional PCR assays that detect Bartonella spp., M. haemofelis, Mhm, Mtc, Rickettsia spp., Ehrlichia spp., Anaplasma spp. and Neorickettsia spp. were performed on DNA extracted from blood and fleas collected from 111 cats. Cat sera were assayed by ELISA for IgG of Bartonella spp.

Results  DNA of M. haemofelis, Mtc and Mhm was amplified from 1 (0.9%), 1 (0.9%) and 17 cats (15.3%), respectively. Only DNA of Mhm was amplified from the 62 of 111 pooled flea samples (flea sets; 55.9%). Overall, the prevalence rates for Bartonella spp. DNA in the cats and the flea sets was 16.2% (18 cats) and 28.8% (32 flea sets), respectively. Bartonella spp. IgG was detected in 42 cats (37.8%), of which 11 (26.2%) were positive for Bartonella spp. DNA in their blood. R. felis DNA was amplified from 22 flea sets (19.8%), but not from cats. Overall, DNA of one or more of the organisms was amplified from 27% (30) of cats and 67.6% (75) of the flea sets.

Conclusions  This is the first Australian study to determine the prevalence of R. felis and B. clarridgeiae in both fleas and the cats from which they were collected. Flea‐associated infectious agents are common in cats and fleas in eastern Australia and support the recommendation that stringent flea control be maintained on cats.

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Australian Veterinary Journal

Tập 88 Số 5

160-165

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