Prevalence and genotype identification of Toxoplasma gondii in suburban rodents collected at waste disposal sites

Parasite (Paris, France) - Tập 26 - Trang 27 - 2019
Vladimir Ivović1, Sandra Potušek1, Elena Bužan1
1Department for Biodiversity, Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, 6000 Koper, Slovenia

Tóm tắt

To assess the prevalence of Toxoplasma gondii infection in native and commensal rodents as indicators of environmental pollution, we analyzed brain tissue from small mammals collected on legal and illegal waste sites in the Slovenian and Croatian parts of Istria. A total of 136 animals and five species of the family Muridae were analyzed: black rat (Rattus rattus), domestic mouse (Mus musculus), wood mouse (Apodemus sylvaticus), striped field mouse (Apodemus agrarius), and yellow-necked mouse (Apodemus flavicollis). Using quantitative Polymerase Chain Reaction (qPCR), T. gondii DNA was detected in four homogenized brain tissue samples (2.94%), from all of the analyzed species, except black rat. Out of these, two samples, domestic mouse (Mus musculus) and wood mouse (Apodemus sylvaticus) had sufficient DNA for genotyping of T. gondii isolates in which we demonstrated the presence of clonal type II using RFLP PCR with four markers (SAG1, SAG2, GRA6 and GRA7). Three of four infected animals (75%) were collected on dumpsites.

Từ khóa


Tài liệu tham khảo

Aramini, 1999, Epidemiology and Infection, 122, 305, 10.1017/S0950268899002113

Berdoy, 2000, Proceedings of the Royal Society of London, 267, 1591, 10.1098/rspb.2000.1182

Berenreiterová, 2011, PLoS One, 6, e28925, 10.1371/journal.pone.0028925

Buzan, 2017, Applied Ecology and Environmental Research: International Scientific Journal, 15, 573, 10.15666/aeer/1501_573588

Dubey, 1995, Journal of Parasitology, 81, 723, 10.2307/3283961

Dubey, 2009, International Journal for Parasitology, 39, 877, 10.1016/j.ijpara.2009.01.005

Duh, 2017, Virology Journal, 14, 134, 10.1186/s12985-017-0798-1

Ferroglio, 2014, Parasites and Vectors, 7, 196, 10.1186/1756-3305-7-196

Galal, 2018, International Journal of Parasitology, 49, 267

Gilot-Fromont E, Lélu M, Dardé ML, Richomme C, Aubert D, Afonso E, Mercier A, Gotteland C, Villena I. 2012. The life cycle of Toxoplasma gondii in the natural environment, in Toxoplasmosis – Recent Advances, Djurkovic-Djakovic O., Editor, InTech, p. 3–36.

Homan, 2000, International Journal for Parasitology, 30, 69, 10.1016/S0020-7519(99)00170-8

Howe, 1997, Journal of Clinical Microbiology, 35, 1411, 10.1128/JCM.35.6.1411-1414.1997

Howe, 1995, Journal of Infectious Diseases, 172, 1561, 10.1093/infdis/172.6.1561

Ivovic, 2015, Acta Veterinaria, 65, 348, 10.1515/acve-2015-0029

Ivović V, Vujanić M, Živković T, Klun I, Djurković-Djaković O. 2012. Molecular detection and genotyping of Toxoplasma gondii from clinical samples, in Toxoplasmosis - Recent Advances, Djurkovic-Djakovic O., Editor, InTech. p. 103–120.

Jones, 2001, American Journal of Epidemiology, 154, 357, 10.1093/aje/154.4.357

Kijlstra, 2008, Veterinary Parasitology, 156, 183, 10.1016/j.vetpar.2008.05.030

Kokalj, 2005, Geografski Vestnik, 52, 12

Mazzanti, 2008, Resources, Conservation and Recycling, 52, 1221, 10.1016/j.resconrec.2008.07.003

Meerburg, 2012, Veterinary Parasitology, 184, 317, 10.1016/j.vetpar.2011.09.001

Pena, 2006, Research in Veterinary Science, 81, 58, 10.1016/j.rvsc.2005.09.007

Reischl, 2003, BMC Infectious Diseases, 3, 7, 10.1186/1471-2334-3-7

Rendón-Franco, 2014, Parasite, 21, 57, 10.1051/parasite/2014058

Rosenthal, 2009, Trends in Parasitology, 25, 67, 10.1016/j.pt.2008.10.004

Rusterholz, 2012, Applied Vegetation Science, 15, 329, 10.1111/j.1654-109X.2011.01175.x

Saki, 2014, BioMed Research International, 2014, 383859, 10.1155/2014/383859

Su, 2010, Parasitology, 137, 1, 10.1017/S0031182009991065

Tenter, 2000, International Journal for Parasitology, 30, 1217, 10.1016/S0020-7519(00)00124-7

Villena, 2004, Applied and Environmental Microbiology, 70, 4035, 10.1128/AEM.70.7.4035-4039.2004

Vujanić, 2011, Vector-Borne and Zoonotic Diseases, 11, 1209, 10.1089/vbz.2010.0119

Worth, 2014, Advances in Parasitology, 85, 109, 10.1016/B978-0-12-800182-0.00003-9