Preparation of radioidinated insulin and thyroid stimulating hormone using 1,3,4,6-tetrachloro-3α, 6α-diphenylglycouril (Iodogen) for radioimmunoassay

Radioiodinated insulin and thyroid stimulating hormones were prepared using 1,3,4,6-tetrachloro-3α, 6α-diphenylglycouril (Iodogen). Conditions of iodination like concentration of iodogen, reaction time, etc., were optimized to get maximum yield. Stability studies of iodogen coated tubes were carried out over a period of time. The dependence of iodination yield on varying amounts of activity and protein concentration were investigated. Iodination yield over a range of pH was also studied. The radiolabelled hormones prepared by this method were used in radioimmunoassay and were compared with tracers prepared by the Chloramine-T method.