Preparation of primary monolayer cultures of adult rat hepatocytes

Berry, M. N.; Friend, D. S. High yield preparation of isolated rat liver parenchymal cells. A biochemical and fine structural study. J. Cell. Biol. 43: 506–520; 1969.

Engelmann, G. L.; Fierer, J. A. Use of exogenous heme to maintain cytochromep-450 content in primary cultures of adult rat hepatocytes. Harris, R. A.; Cornell, N. W. eds. Isolation, characterization and use of hepatocytes. Elsevier; New York. 1983: 117–122.

Bissell, D. M. Primary hepatocyte culture: Substratum requirements and production of matrix components. Fed. Proc. 40: 2469–2473; 1981.

Seglen, P. O.; Fossa, J. Attachment of rat hepatocytes in vitro to substrata of serum protein, collagen, or conconavalin A. Exp. Cell Res. 116: 119–206; 1978.

Schwarze, P. E.; Solheim, A. E.; Seglen, P. O. Amino acid and energy requirements for rat hepatocytes in primary culture. In Vitro 18: 43–54; 1982.

Seglen, P. O.; Solheim, A. E.; Grinde, B.; Gordon, P. B.; Schwarze, P. E.; Gjessing, R.; Pol, A. Amino acid control of protein synthesis and degradation in isolated rat hepatocytes. Ann. N. Y. Acad. Sci. 349: 1–17; 1980.

Decad, G. M.; Hsieh, D. P. H.; Byard, J. L. Maintenance of cytochromep-450 and metabolism of aflatoxin B1 primary hepatocyte cultures. Biochem. Biophys. Res. Commun. 78: 279–287; 1977.

Paine, A. J.; Williams, L. J.; Legg, R. F. Apparent maintenance of cytochromep-450 by nicotinamide in primary cultures of rat hepatocytes. Life Sci. 24: 2185–2192; 1979.

Omaye, S. T.; Turnbull, J. D. Effect of ascorbic acid on heme metabolism in hepatic microsomes. Life Sci. 27: 441–449; 1980.

Paine, A. J.; Hockin, L. J. Nutrient imbalance causes the loss of cytochromep-450 in liver cell culture: Formulation of culture media which maintains cytochromep-450 at in vivo concentrations. Biochem. Pharmacol. 29: 3215–3218; 1980.

Guzelian, P. S.; Bissell, D. M. Effect of cobalt on synthesis of heme and cytochromep-450 in the liver. Studies of adult rat hepatocytes in primary monolayer culture and in vitro. J. Biol. Chem. 251: 4421–4427; 1976.

Newman, S.; Guzelian, P. S. Stimulation of the de novo synthesis of cytochromep-450 by phenobarbital in primary non-proliferating cultures of adult rat hepatocytes. Proc. Natl. Acad. Sci. USA 79: 2922–2926; 1982.

Dougherty, K. K.; Spilman, S. D.; Green, C. E.; Steward, A. R.; Byard, J. L. Primary cultures of adult mouse and rat hepatocytes for studying the metabolism of foreign chemicals. Biochem. Pharmacol. 29: 2117–2124; 1980.