Preparation of aptamer-linked gold nanoparticle purple aggregates for colorimetric sensing of analytes

Nature Protocols - Tập 1 Số 1 - Trang 246-252 - 2006
Juewen Liu1, Yi Lu1
1Department of Chemistry, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, USA

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Tài liệu tham khảo

Ellington, A.D. & Szostak, J.W. In vitro selection of RNA molecules that bind specific ligands. Nature 346, 818–822 (1990).

Tuerk, C. & Gold, L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249, 505–510 (1990).

Wilson, D.S. & Szostak, J.W. In vitro selection of functional nucleic acids. Annu. Rev. Biochem. 68, 611–647 (1999).

Famulok, M. Oligonucleotide aptamers that recognize small molecules. Curr. Opin. Struct. Biol. 9, 324–329 (1999).

Conrad, R.C., Giver, L., Tian, Y. & Ellington, A.D. In vitro selection of nucleic acid aptamers that bind proteins. Methods Enzymol. 267, 336–367 (1996).

Cox, J.C. & Ellington, A.D. Automated selection of anti-protein aptamers. Bioorg. Med. Chem. 9, 2525–2531 (2001).

Mendonsa, S.D. & Bowser, M.T. In vitro evolution of functional DNA using capillary electrophoresis. J. Am. Chem. Soc. 126, 20–21 (2004).

Berezovski, M. et al. Nonequilibrium capillary electrophoresis of equilibrium mixtures: a universal tool for development of aptamers. J. Am. Chem. Soc. 127, 3165–3171 (2005).

Nutiu, R. & Li, Y. Structure-switching signaling aptamers. J. Am. Chem. Soc. 125, 4771–4778 (2003).

Levy, M., Cater, S.F. & Ellington, A.D. Quantum-dot aptamer beacons for the detection of proteins. ChemBioChem 6, 2163 (2005).

Famulok, M., Mayer, G. & Blind, M. Nucleic acid aptamers—from selection in vitro to applications in vivo. Acc. Chem. Res. 33, 591–599 (2000).

Liu, J. & Lu, Y. Fast colorimetric sensing of adenosine and cocaine based on a general sensor design involving aptamers and nanoparticles. Angew. Chem. Int. Ed. Engl. 45, 90 (2006).

Stojanovic, M.N. & Landry, D.W. Aptamer-based colorimetric probe for cocaine. J. Am. Chem. Soc. 124, 9678–9679 (2002).

Pavlov, V., Xiao, Y., Shlyahovsky, B. & Willner, I. Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin. J. Am. Chem. Soc. 126, 11768–11769 (2004).

Huang, C.-C., Huang, Y.-F., Cao, Z., Tan, W. & Chang, H.-T. Aptamer-modified gold nanoparticles for colorimetric determination of platelet-derived growth factors and their receptors. Anal. Chem. 77, 5735–5741 (2005).

Liu, J. & Lu, Y. A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles. J. Am. Chem. Soc. 125, 6642–6643 (2003).

Liu, J. & Lu, Y. Stimuli-responsive disassembly of nanoparticle aggregates for light-up colorimetric sensing. J. Am. Chem. Soc. 127, 12677–12683 (2005).

Mirkin, C.A., Letsinger, R.L., Mucic, R.C. & Storhoff, J.J. A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 382, 607–609 (1996).

Hermann, T. & Patel, D.J. Adaptive recognition by nucleic acid aptamers. Science 287, 820–825 (2000).

Nutiu, R. & Li, Y. Structure-switching signaling aptamers: transducing molecular recognition into fluorescence signaling. Chem. Eur. J. 10, 1868–1876 (2004).

Storhoff, J.J., Elghanian, R., Mucic, R.C., Mirkin, C.A. & Letsinger, R.L. One-pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes. J. Am. Chem. Soc. 120, 1959–1964 (1998).

Handley, D.A. Methods for synthesis of colloidal gold. in Colloidal Gold Principles, Methods, and Applications, Vol. 1 (ed. Hayat, M.A.) 13–32 (Academic Press, San Diego, 1989).

Zuker, M. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 31, 3406–3415 (2003).