Chuẩn bị và đánh giá pha tĩnh phenylglycine zwitterionic liên kết bề mặt

Springer Science and Business Media LLC - Tập 410 - Trang 5941-5950 - 2018
Huanjun Peng1, Xiang Wang1, Jingdong Peng1, Yan He1, Yu Chen1, Fang Chen1, Shiyu Li1
1College of Chemistry and Chemical Engineering, Southwest University (SWU), Chongqing, China

Tóm tắt

4-Hydroxy-d-phenylglycine đã được chỉnh sửa bằng anhydride methacrylic và sau đó được cố định trên silica thông qua polymer hóa bề mặt khởi đầu bằng thiol; vật liệu đã chuẩn bị được sử dụng làm pha tĩnh cho HPLC. Pha tĩnh thu được đã được đặc trưng hóa bằng phân tích nguyên tố, phổ hồng ngoại và phân tích nhiệt khối lượng. Hiệu suất sắc ký của cột nhồi được đánh giá trong chế độ sắc ký lỏng đảo pha (RPLC) và sắc ký lỏng tương tác ưa nước (HILIC); cột này đã cho thấy độ chọn lọc xuất sắc đối với cả chất hòa tan ưa nước và ưa nước. Độ chọn lọc đối với các hydrocarbon thơm đa vòng so với alkylbenzenes được cột đã chuẩn bị thể hiện cao hơn độ chọn lọc tương ứng của cột C18 thương mại, điều này có thể được giải thích bằng tương tác π-π điện tử giữa phenylglycine và các vòng thơm giàu electron. Mặt khác, cột đã chuẩn bị cũng cho thấy độ chọn lọc tốt hơn cho các hợp chất phân cực, điều này dựa trên nhiều cơ chế tương tác và giữ lại. Nó cũng được sử dụng để tách sulfonamide và acid hữu cơ so với cột C18 và HILIC thương mại; kết quả cho thấy hiệu suất sắc ký tuyệt vời với độ chọn lọc đặc biệt. Tất cả các kết quả chỉ ra rằng cột đã chuẩn bị có khả năng ứng dụng trong nhiều lĩnh vực.

Từ khóa

#pha tĩnh #phenylglycine #sắc ký lỏng #tương tác ưa nước #hydrocarbon thơm đa vòng

Tài liệu tham khảo

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