Preliminary assessment of protein associated with airborne particles in Mexico City
Tóm tắt
The protein associated with airborne particles was measured during 1991 as an indicator of airborne biological material in different outdoor urban environments. Fifty air samples were collected simultaneously at three sampling sites, located in the north, south and downtown Mexico City, using a PM10 high-volume sampler (particles<10 μm). The air filters were weighed and protein extracted using a phosphate buffer. Protein concentrations were determined by Lowry assay. The extracts were also analysed by SDS electrophoresis and IEF using a Phastsystem. High concentrations of airborne particles were recorded at the sampling sites with a geometric mean of 70.2 μg/m3 in the south (residential area), 95.5 μg/m3 in the center (urban-commercial area), and with the highest value of 108.9 μg/m3 in the north (urban-industrial area). No statistically significant difference (P>0.05) was observed among the protein concentrations from the sampling sites and the concentrations ranged from non-detectable to 2.54 μg/m3. However, the protein concentrations presented significant difference (P<0.05) with respect to rainy and dry seasons. The Spearman correlation coefficient between protein concentration and airborne particles concentration was statistically significant (r=0.50). The molecular weights (MW) and isoelectric points (pI) for the proteins present in some of the extracts were determined. The values ranged from approximately 8000 to 106 000 Da and the pI values from nearly 4.0 to 5.85. This is important because the major allergens from inhalants are mostly acidic proteins with molecular weights in the range of 20 000–40 000 Da.
Tài liệu tham khảo
Agarwal, M., Yunginger, J., Swanson, M. and Reed, C. (1981) An immunochemical method to measure atmospheric allergens. J. Allergy Clin. Immunol. 68, 194–200.
Ansari, A., Shenbagamurthi, P. and Marsh, D. (1989) Complete amino acid sequence of aLolium perenne (perennial rye grass) pollen allergen, Lol p II. J. Biol. Chem. 264, 11181–11183.
Benjamin, D., Berzofsky, J., East, J., Gurd, F., Hannum, C., Leach, S., Margoliash, E., Michael, J., Miller, A., Prager, E., Reichlin, M., Sercarz, E., Smith-Gill, S., Todd, P. and Wilson, A. (1984) The antigenic structure of proteins: a reappraisal. Annu. Rev. Immunol. 2, 67–101.
Báez, A., Padilla, H. and Belmont, R. (1993) Seavenging of atmospheric formaldehyde by wet precipitation. Environ. Pollut. 79, 271–275.
Calderón-Garcidueñas, L., Osorno-Velázquez, A., Bravo, H., Delgado-Chávez, R. and Barrios-Márquez, R. (1992) Histopathologic changes on the nasal mucosa in southwest metropolitan Mexico City inhabitants. Am. J. Pathol. 140, 225–232.
Calderón, M., Lacey, C. and Rosas, I. (1993) Seasonal variation of basidiospores in the atmosphere of México City. Aerobiology 1993, Symposium of the Pan-American Aerobiology Association, Program and summary, 19–21 May 1993.
Chapman, J. (1986) Aeroallergens of southeastern Missouri, USA. Grana 25, 235–246.
González-Macias, C., González-Lozano, C., Salazar, L. and Rosas, I., (1993) A one year pollen monitoring in the atmosphere of the México City Metropolitan area. Aerobiology 1993, Symposium of the Pan-American Aerobiology Association, Program and summary, 19–21 May 1993.
Hamilton, R., Chapman, M., Platts-Mills, T. and Adkinson, N. (1992) House dust aeroallergen measurements in clinical practice: a guide to allergen-free home and work environments. Immunol. Allergy Pract. 14, 96–112.
Hoaglin, D., Mosteller, F. and Tukey, J. (1983) Understanding Robust and Exploratory Data Analysis. Wiley, New York.
Hyde, H. (1973) Atmospheric pollen grains and spores in relation to allergy. II. Clin. Allergy 3, 109–126.
King, T. (1976) Chemical and biological properties of some atopic allergens. Adv. Immunol. 23, 77–105.
Lowry, O., Rosebrough, N., Farr, A. and Randall, R. (1951) Protein measurements with the Folin reagent. J. Biol. Chem. 93, 265–275.
McFarland, A., Ortiz, C. and Bertch, Jr., R. (1984) A 10 μm cutpoint size selective inlet for Hi-Vol Samplers. J. Air Pollut. Contr. Asoc. 34, 544–547.
Oke, T. and Zeuner, G. (1992) The surface energy balance in México City. Atmos. Environ. 26B, 433–444.
O’Rourke, M., Quackenboss, J. and Lebowitz, M. (1989) An epidemiological approach investigating respiratory disease response in sensitive individuals to indoor and outdoor pollen exposure in Tucson, Arizona. Aerobiologia 5, 104–110.
Pharmacia-LKB Biotechnology, IEF and Electrophoretic Titration Curves. Phast System Separation Technique File No. 100. Pharmacia-LKB Biotechnology, Uppsala, Sweden.
Pope III, C.A. (1991) Respiratory hospital admissions associated with PM10 pollution in Utah, Salt Lake and Cache Valleys. Arch. Environ. Health 46, 90–97.
Preller, L., Hollander, A., Heederik, D. and Brunekreet, B. (1989) Potentially allergenic airborne particles in the vicinity of the yeast and Penicillium production plant. J. Air Pollut. Contr. Assoc. 39, 1094–1097.
Rapoport, H., Díaz-Betancurt, E. and López-Moreno, R. (1983) Aspectos de la ecología urbana de la ciudad de México. Ed. Limusa, México City.
Salazar, S., Lemoyne, L. and Rosas, I. (1992) Preliminary results of size distribution airborne particles in Mexico City. Water Air Soil Pollut. 64, 649–658.
Schneeberger, E. (1974) The permeability of the alveolar capillary membrane to ultrastructural protein tracers. Ann. New York Acad. Sci. 221, 238–244.
Schumacher, M. (1980) Characterization of allergens from urine and pelts of laboratory mice. Mol. Immunol. 17, 1087–1095.
Solomon, A., Fall, T., Salmon, L. and Cass, G. (1989) Chemical characteristics of PM10 aerosol collected in the Los Angeles area. J. Air Pollut. Contr. Assoc. 29, 154–163.
Taylor, A., Longbottom, J. and Pepys, J. (1977) Respiratory allergy to urine proteins of rats and mice. Lancet 2, 847–849.
Tovey, E., Chapman, M. and Platts-Mills, T. (1981) Mite faeces are a major source of house dust allergens. Nature 289, 592–593.
Twiggs, J., Agarwal, M., Dahlberg, M. and Yunginger, J. (1982) Immunochemical measurement of airborne mouse allergens in a laboratory animal facility. J. Allergy Clin. Immunol. 69, 522–526.
Yunginger, J., Jones, R., Nesheim, M. and Geller, M. (1980) Studies onAlternaria allergens. III. Isolation of a major allergenic fraction (Alt-l). J. Allergy Clin. Immunol. 66, 138–147.