Preliminary Assessment of Resistance of Potato Varieties to Potato latent virus

American Potato Journal - Tập 87 - Trang 78-82 - 2009
Xianzhou Nie1
1Potato Research Centre, Agriculture and Agri-Food Canada, Fredericton, Canada

Tóm tắt

Sixty-one potato cultivars were assessed for their infectability with Potato latent virus (PotLV). Fifty eight were detected positive for PotLV by reverse transcription-polymerase chain reaction upon one or two mechanical inoculations. Virus was not detected in cultivars Caribe, Jemseg and Saco even after being mechanically inoculated three times. Secondary infection, which was assessed on the progeny plants by enzyme-linked immunosobent assay, was detected in all cultivars but Caribe, Jemseg and Saco. The three cultivars were then subjected to graft-inoculation with PotLV. Although detectable in the recipient rootstocks at 21 days post inoculation in all three cultivars, PotLV titer was lower in rootstocks than in scions. The same was true for tubers harvested from the graft-inoculated plants. Interestingly, progeny plants emerged from these tubers were PotLV-free. The results suggest that host resistance to PotLV exists in Caribe, Jemseg and Saco.

Tài liệu tham khảo

Akeley, E.V., F.J. Stevenson, E.S. Schultz, R. Bonde, K.F. Nielsen, and A. Hawkins. 1955. Saco: A new late-maturing variety of potato, immune from common race of the late blight fungus, highly resistant to, if not immune from net necrosis, and immune from mild and latent mosaics. American Potato Journal 32: 41–48. Brattey, C., J.L. Badge, R. Burns, G.D. Foster, E. George, H.A. Goodfellow, V. Mulholland, J.G. McDonald, and C.J. Jeffries. 2002. Potato latent virus: A proposed new species in the genus Carlavirus. Plant Pathology 51: 495–505. Goth, R.W. and E.W. Goins. 1998. Distribution of Carlavirus Red LaSoda (RLSV). American Journal of Potato Research 75: 277. Goth, R.W., P.J. Ellis, G. de Villiers, E.W. Goins, and N.S. Wright. 1999. Characteristics and distribution of potato latent carlavirus (Red LaSoda virus) in North America. Plant Disease 83: 751–753. Massa, G.A., M.E. Segretin, M. Colavita, M.F. Riero, F. Bravo-Almonacid, and S. Feingold. 2006. Biological and sequence data suggest that potato rough dwarf virus (PRDV) and potato virus P (PVP) are strains of the same species. Archives of Virology 151: 1243–1247. NAPPO. 2003. Regional Standard for Phytosanitary Measures (RSPM): RSPM No. 3 - Requirements for importation of potatoes into a NAPPO member country. Nie, X. 2009. The complete nucleotide sequence and genome structure of Potato latent virus. Archives of Virology 154: 361–364. Nie, X., Y. Bai, T.A. Molen, and D.C. Desjardins. 2008. Development of universal primers for detection of potato carlaviruses by RT-PCR. Journal of Virology of Methods 149: 209–216. Nisbet, C., I. Butzonitch, M. Colavita, J. Daniels, J. Martin, R. Burns, E. George, M.A.Y. Akhond, V. Mulholland, and C.J. Jeffries. 2006. Characterization of potato rough dwarf virus and potato virus P: Distinct strains of the same viral species in the genus Carlavirus. Plant Pathology 55: 803–812. Singh, R.P., X. Nie, M. Singh, R. Coffin, and P. Duplessis. 2002. Sodium sulphite inhibition of potato and chery polyphenolics in nucleic acid extraction for virus detection by RT-PCR. Journal of Virological Methods 99: 123–131. Young, D.A. and H. De Jong. 1984. Caribe: a smooth, oblong, purple-skinned potato variety of early maturity. American Potato Journal 61: 281–286. Young, D.A., H.T. Davies, and G.R. Johnston. 1979. Jemseg: A new, early high-yielding potato variety with high resistance to virus Y and immune to virus X. American Potato Journal 56: 325–328.