Precise droplet volume measurement and electrode-based volume metering in digital microfluidics

Microfluidics and Nanofluidics - Tập 17 Số 2 - Trang 295-303 - 2014
Liu, Yuguang1, Banerjee, Ananda1, Papautsky, Ian1
1Department of Electrical Engineering and Computing Systems, University of Cincinnati, Cincinnati, USA

Tóm tắt

In this work, for the first time, we demonstrate nanoscale droplet generation from a continuous electrowetting microchannel using a simple and precise image-based droplet volume metering technique. One of the most popular ways of droplet generation in electrowetting devices is to split a droplet from a preloaded volume as a fluid reservoir. This method is effective, but lowers volume consistency after multiple droplets are generated. Impedance- and capacitance-based methods of volume metering have been successfully used in digital microfluidics, but require complex circuitry and feedback signal processing. In this work, we demonstrate nanoliter droplet generation from a continuous electrowetting channel used as a replenishable fluid reservoir which compensates for the loss of reservoir volume as droplets are sequentially split. This improves volume consistency especially for applications requiring multi-droplet generation. Based on the area of the electrode, the volume of each droplet split from the electrowetting channel can be obtained by a simple and precise image processing technique with no need for additional hardware and measurement errors of ±0.05 %. This simple technique can be used in a wide range of applications that require precise volume metering, such as immunoassay.

Tài liệu tham khảo

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