Pre-ischemic exercise reduces apoptosis in hippocampal CA3 cells after cerebral ischemia by modulation of the Bax/Bcl-2 proteins ratio and prevention of caspase-3 activation

The Journal of Physiological Sciences - Tập 65 - Trang 435-443 - 2015
Nahid Aboutaleb1, Nabi Shamsaei2, Mehdi Khaksari3, Sohaila Erfani4, Hamid Rajabi5, Farnaz Nikbakht1
1Physiology Research Center and Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
2Department of Physical Education and Sports Science, Faculty of Literature and Humanities, Ilam University, Ilam, Iran
3School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
4Department of Animal Physiology, Faculty of Biology, Kharazmi University, Tehran, Iran
5Faculty of Physical Education and Sports Science, Kharazmi University, Tehran, Iran

Tóm tắt

Ischemia induces physiological alterations in neurons that lead to cell death. This study investigated the effects of pre-ischemic exercise on CA3 neurons. Rats were divided into three groups. Animals in the exercise group were trained 5 days a week for 4 weeks. Ischemia was induced by occlusion of both common carotid arteries (CCAs) for 20 min. Apoptotic cell death was detected by TUNEL assay. Furthermore, expression of different proteins was determined by immunohistochemical staining. The number of TUNEL-positive cells was significantly increased in the ischemia group, but pre-ischemic exercise significantly reduced apoptotic cell death (P < 0.001). In addition, our results showed a significant increase in the Bax/Bcl-2 ratio in the ischemia group. Pre-ischemic exercise attenuated this ratio (P < 0.05). Furthermore, the number of active caspase-3-positive neurons was significantly increased in the ischemia group, which was reduced markedly by exercise preconditioning (P < 0.05). This study showed that pre-ischemic exercise can exert neuroprotective effects against ischemia in CA3 neurons.

Tài liệu tham khảo

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