Potassium transporter KUP9 participates in K+ distribution in roots and leaves under low K+ stress

Taro Yamanashi1, Takeshi Uchiyama1, Shunya Saito1, Tomoko Higashi1, Hayato Ikeda2, H. Kikunaga2, Mutsumi Yamagami3, Yasuhiro Ishimaru1, Nobuyuki Uozumi1
1Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan
2Research Center for Electron Photon Science, Tohoku University, Sendai, 980-0826, Japan
3Institute for Environmental Sciences, Rokkasho, Kamikita, Aomori, 039-3212, Japan

Tóm tắt

AbstractPotassium (K) is a major essential element in plant cells, and KUP/HAK/KT-type K+ transporters participate in the absorption of K+ into roots and in the long-distance transport to above-ground parts. In Arabidopsis thaliana, KUP9 is involved in the transport of K+ and Cs+ in roots. In this study, we investigated KUP9 function in relation to the K+ status of the plant. The expression of KUP9 was upregulated in older leaves on K+-depleted medium, compared to the expression of the other 12 KUP genes in the KUP/HAK/KT family in Arabidopsis. When grown on low K+ medium, the kup9 mutant had reduced chlorophyll content in seedlings and chlorosis in older rosette leaves. Tissue-specific expression of KUP9 determined by KUP9 promoter:GUS assay depended on the K+ status of the plants: In K+ sufficient medium, KUP9 was expressed in the leaf blade towards the leaf tip, whereas in K+ depleted medium expression was mainly found in the petioles. In accordance with this, K+ accumulated in the roots of kup9 plants. The short-term 43K+ tracer measurement showed that 43K was transferred at a lower rate in roots and shoots of kup9, compared to the wild type. These data show that KUP9 participates in the distribution of K+ in leaves and K+ absorption in roots under low K+ conditions.

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