Polymorphism of storage proteins in accessions of pea wild taxa
Tóm tắt
Electrophoretic spectra of storage proteins in wild taxa of pea have been studied. Composition of protein bands essential for identification of biochemical markers of introgressive alleles with agronomic value has been described. Cluster analysis has been performed with use of the UPMGA algorithm. Two clusters have been determined on a dendrogram with high level of bootstrap index (BI = 99.3%). P. fulvum accessions I609881, I609885, 2523, and 6070 have been placed in different clusters. This fact points to heterogeneity of the species under study and possibility of interspecific introgressions during ancient times. Accessions of P. fulvum 2523 and 6070 have been characterized by a higher number of isoforms of convicilin. Differences between the accessions of P. fulvum should be taken into consideration during interspecific hybridization.
Tài liệu tham khảo
Gupta, A.J., Singh, Y.V., and Rarn, H.H., Seed protein profiles and cultivar identification in garden pea (Pisum sativum L.), Indian J. Genet. Plant Breed., 2008, vol. 8, no. 3, pp. 283–287.
Nisar, M., Ghafoor, A., Rashid Khan, M., and Asmatullah, First proteomic assay of Pakistani Pisum sativum L. germplasm relation to geographic pattern, Genetika (Moscow), 2009, vol. 45, no. 7, pp. 920–925.
Makasheva, R.Kh., Grain leguminous cultures, in Kul’turnaya flora SSSR (Cultured Flora of USSR), St. Petersburg: Kolos, 1979, pp. 45–49.
Fondevilla, S., et al., Response of Micosphaerella pinodes in a germplasm collection, Plant Breed., 2005, vol. 124, pp. 313–315.
Byrne, O.M. Hardie, D.C., Kahn, T.N., et al., Genetic analysis of pod and seed resistance to pea weevil in a Pisum sativum × P. fulvum interspecific cross, Aust. J. Agric. Res., 2008, vol. 59, pp. 854–862.
Fondevilla, S. Cubero, J.I., and Rubiales, D., Confirmation that the Er3 gene, conferring resistance to Erysiphe pisi in pea, is a different gene from er1 and er2 genes, Plant Breed., 2010.
Jha, S.S. and Ohri, D., Comparative study of seed protein profiles in the genus Pisum, Biol. Plant, 2002, vol. 45, no. 4, pp. 529–532.
Zelenov, A.N., Shchetinin, V.Yu., Kondykov, I.V., Uvarov, V.N., Zadorin, A.M., Borzenkova, G.A., Azarova, E.F., Naumkina, T.S., Bobkov, S.V., and Uvarova, O.V., Pea (Pisum sativum L.) — the forms with changed leaf structure, in Pasporta donorov i istochnikov selektsionno-tsennykh priznakov sel’skokhozyaistvennykh kul’tur (Donor’s Certificates and Sources of Valuable Breeding Features of Agricultural Plant Cultures), Orel, 2011, no. 9.
Konarev, V.G., et al., Identifikatsiya sortov i registratsiya genofonda kul’turnykh rastenii po belkam (Identification of the Cultivars and Registration of the Gene Pool of Agricultural Plants According to Seed Proteins), Konarev, V.G., et al., Eds., St. Petersburg: Vseross. Inst. Rastenievod., 2000.
van de Peer, Y. and de Wachter, Y., TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment, CABIOS, Comput. Appl. Biosci., 1994, vol. 10, pp. 569–570.
Tzitzikas, E.N., Vincken, J.P., Groot, J., et al., Genetic variation in pea seed composition, J. Agric. Food Chem., 2006, no. 54, pp. 425–433.
Bobkov, S.V. and Lazareva, T.N., Band composition of electrophoretic spectra of storage proteins in interspecific pea hybrids, Russ. J. Genet., 2012, vol. 48, no. 1, pp. 47–52.