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Cảm biến sinh học dựa trên nanocomposite polymer – đất sét acetylcholinesterase cho việc phát hiện thuốc trừ sâu phospho hữu cơ
Tóm tắt
Trong nghiên cứu này, một nanocomposite chitosan (CH) – đất sét montmorillonite (MT) đã được chuẩn bị thông qua sự phân tán đồng nhất các hạt MT trong dung dịch CH đồng nhất và được lắng đọng lên bề mặt kính phủ oxit indium thiếc (ITO). Các kỹ thuật nhiễu xạ tia X (XRD), kính hiển vi điện tử truyền qua (TEM), kính hiển vi điện tử quét, phổ hồng ngoại biến đổi Fourier và các kỹ thuật điện hóa đã được sử dụng để đặc trưng hóa nanocomposite CH–MT. Kết quả XRD và TEM xác nhận sự tách lớp và chèn MT vào ma trận CH. Nanocomposite CH–MT này đã được sử dụng để chế tạo cảm biến sinh học enzym thông qua việc cố định acetylcholinesterase (AChE) cho việc phát hiện thuốc trừ sâu phospho hữu cơ (chlorpyriphos). Kết quả cho thấy hệ số khuếch tán, tốc độ truyền điện tử và nồng độ bề mặt đã tăng lên sau khi cố định AChE lên điện cực CH–MT/ITO. Điện cực sinh học AChE/CH–MT/ITO cho thấy khoảng tuyến tính từ 0,5 đến 1000 µg L−1 với giới hạn phát hiện (LOD) là 0,448 µg L−1 và độ nhạy là 69,6 µA mM−1 cm2. Hằng số Michaelis–Menten biểu kiến (
$$K_{\text{m}}^{\text{app}}$$
) thu được là 0,219 mM như một hàm của chlorpyriphos. Điện cực sinh học được chế tạo có thể là một nền tảng tuyệt vời cho việc phát hiện chlorpyriphos.
Từ khóa
#nanocomposite #chitosan #montmorillonite #acetylcholinesterase #cảm biến sinh học #thuốc trừ sâu #chlorpyriphosTài liệu tham khảo
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