Pollination intensity and nutrient availability interactively shape directional selection on flower size in an orchid herb

Ashman T-L, Morgan MT (2004) Explaining phenotypic selection on plant attractive characters: male function, gender balance or ecological context? P Roy Soc B 271:553–559 Ashman T-L, Knight TM, Steets JA et al (2004) Pollen limitation of plant reproduction: ecological and evolutionary causes and consequences. Ecology 85:2408–2421 Benkman CW (2013) Biotic interaction strength and the intensity of selection. Ecol Lett 16:1054–1060 Bergman P, Molar U, Holmgren B (1996) Micrometeorological impacts on insect activity and plant reproductive success in an alpine environment, Swedish Lapland. Arct Alp Res 28:196–202 Brookes RH, Jesson LK, Burd M (2008) A test of simultaneous resource and pollen limitation in Stylidium armeria. New Phytol 179:557–565 Campbell DR, Bischoff M (2013) Selection for a floral trait is not mediated by pollen receipt even though seed set in the population is pollen-limited. Funct Ecol 27:1117–1125 Campbell DR, Halama KJ (1993) Resource and pollen limitations to lifetime seed production in a natural plant population. Ecology 74:1043–1051 Campbell DR, Powers JM (2015) Natural selection on floral morphology can be influenced by climate. P Roy Soc B 282:20150178 Caruso CM, Peterson SB, Ridley CE (2003) Natural selection on floral traits of Lobelia (Lobeliaceae): spatial and temporal variation. Am J Bot 90:1333–1340 Caruso CM, Remington DLD, Ostergren KE (2005) Variation in resource limitation of plant reproduction influences natural selection on floral traits of Asclepias syriaca. Oecologia 146:68–76 Chapurlat E, Ågren J, Sletvold N (2015) Spatial variation in pollinator-mediated selection on phenology, floral display and spur length in the orchid Gymnadenia conopsea. New Phytol 208:1264–1275 Crow JF (1958) Some possibilities for measuring selection intensities in man. Hum Biol 30:1–13 Cuartas-Domínguez M, Medel R (2010) Pollinator-mediated selection and experimental manipulation of the flower phenotype in Chloraea bletioides. Funct Ecol 24:1219–1227 Donner A, Eliasziw M (1987) Sample size requirements for reliability studies. Stat Med 6:441–448 Downhower JF, Blumer LS, Brown L (1987) Opportunity for selection: an appropriate measure for evaluating variation in the potential for selection? Evolution 41:1395–1400 Emel SL, Franks SJ, Spigler RB (2017) Phenotypic selection varies with pollination intensity across populations of Sabatia angularis. New Phytol 215:813–824 Friberg M, Waters MT, Thompson JN (2017) Nutrient availability affects floral scent much less than other floral and vegetative traits in Lithophragma bolanderi. Ann Bot 120:471–478 Haig D, Westoby M (1988) On limits to seed production. Am Nat 131:757–759 Hodgins KA, Barrett SCH (2008) Natural selection on floral traits through male and female function in wild populations of the heterostylous daffodil Narcissus triandrus. Evolution 62:1751–1763 Iwata T, Nagasaki O, Ishii HS et al (2012) Inflorescence architecture affects pollinator behaviour and mating success in Spiranthes sinensis (Orchidaceae). New Phytol 193:196–203 Lande R, Arnold SJ (1983) The measurement of selection on correlated characters. Evolution 37:1210–1226 Mattila E, Kuitunen MT (2000) Nutrient versus pollination limitation in Platanthera bifolia and Dactylorhiza incarnata (Orchidaceae). Oikos 89:360–366 Minnaar C, Anderson B, de Jager ML et al (2019) Plant-pollinator interactions along the pathway to paternity. Ann Bot 123:225–245 Mitchell RJ, Karron JD, Holmquist KG et al (2004) The influence of Mimulus ringens floral display size on pollinator visitation patterns. Funct Ecol 18:116–124 O’Donald P (1970) Change in fitness by selection for a quantitative character. Theor Popul Biol 1:219–232 Øien DI, Pedersen B (2003) Seasonal pattern of dry matter allocation in Dactylorhiza lapponica (Orchidaceae) and the relation between tuber size and flowering. Nord J Bot 23:441–451 Parachnowitsch AL, Kessler A (2010) Pollinators exert natural selection on floral size and floral display in Penstemon digitalis. New Phytol 188:393–402 Quinn GP, Keough MJ (2002) Experimental design and data analysis for biologists. Cambridge University Press, New York R Core Team (2019) R: a language and environment for statistical computing, R Foundation for Statistical Computing, Vienna, http://www.r-project.org Sandring S, Ågren J (2009) Pollinator-mediated selection on floral display and flowering time in the perennial herb Arabidopsis lyrata. Evolution 63:1292–1300 Sandring S, Riihimäki M-A, Savolainen O et al (2007) Selection on flowering time and floral display in an alpine and a lowland population of Arabidopsis lyrata. J Evol Biol 20:558–567 Sletvold N, Ågren J (2016) Experimental reduction in interaction intensity strongly affects biotic selection. Ecology 97:3091–3098 Sletvold N, Tye M, Ågren J (2017) Resource- and pollinator-mediated selection on floral traits. Funct Ecol 31:135–141 Trunschke J, Sletvold N, Ågren J (2017) Interaction intensity and pollinator-mediated selection. New Phytol 214:1381–1389 Vanhoenacker D, Ågren J, Ehrlén J (2013) Non-linear relationship between intensity of plant-animal interactions and selection strength. Ecol Lett 16:198–205 Weis AE, Abrahamson WG, Andersen MC (1992) Variable selection on Eurosta’s gall size, I: the extent and natural of variation in phenotypic selection. Evolution 46:1674–1697 Wilson P (1995) Variation in the intensity of pollination in Drosera tracyi: selection is strongest when resources are intermediate. Evol Ecol 9:382–396 Wu Y, Duan XY, Xiang Y et al (2020) Pollinator-dependent evolution of floral trait combinations in an orchid herb. J Plant Ecol 13:450–459 Wu Y, Duan XY, Tong ZL et al (2022) Pollinator-mediated selection on floral traits of Primula tibetica differs between sites with different soil water contents and among different levels of nutrient availability. Front Plant Sci 13:807689