Plant stress granules and mRNA processing bodies are distinct from heat stress granules

Plant Journal - Tập 56 Số 4 - Trang 517-530 - 2008
Christian Weber1, Lutz Nover2, Markus Fauth3
1Present address: Institute of Molecular Biosciences, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany.
2Department of Molecular Cell Biology, Johann Wolfgang Goethe-University Frankfurt, Max von Laue Straße 9, 60438 Frankfurt, Germany
3Present address: Markus Fauth, Institute of Molecular Biosciences, Johann-Wolfgang Goethe University Frankfurt, Siesmayerstraße 70, 60323 Frankfurt, Germany.

Tóm tắt

SummarySimilar to the situation in mammalian cells and yeast, messenger ribonucleo protein (mRNP) homeostasis in plant cells depends on rapid transitions between three functional states, i.e. translated mRNPs in polysomes, stored mRNPs and mRNPs under degradation. Studies in mammalian cells showed that whenever the dynamic exchange of the components between these states is disrupted, stalled mRNPs accumulate in cytoplasmic aggregates, such as stress granules (SGs) or processing bodies (PBs). We identified PBs and SGs in plant cells by detection of DCP1, DCP2 and XRN4, as marker proteins for the 5′→3′ mRNA degradation pathway, and eIF4E, as well as the RNA binding proteins RBP47 and UBP1, as marker proteins for stored mRNPs in SGs. Cycloheximide‐inhibited translation, stress treatments and mutants defective in mRNP homeostasis were used to study the dynamic transitions of mRNPs between SGs and PBs. SGs and PBs can be clearly discriminated from the previously described heat stress granules (HSGs), which evidently do not contain mRNPs. Thus, the role of HSGs as putative mRNP storage sites must be revised.

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Plant Journal

Tập 56 Số 4

517-530

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