Phản ứng của phù du đối với bức xạ UV mặt trời và sự kết hợp với việc bổ sung dinh dưỡng ở hồ Fuxian oligotrophic trên cao nguyên: một thí nghiệm mesocosm

Springer Science and Business Media LLC - Tập 28 - Trang 29931-29944 - 2021
Yun Zhang1, Kaidi Li1,2, Qichao Zhou1,2, Le Chen1,2, Xuan Yang1, Hucai Zhang1
1Institute for Ecological Research and Pollution Control of Plateau Lakes, School of Ecology and Environmental Sciences, Yunnan University, Kunming, China
2Yunnan Key Laboratory of Pollution Process and Management of Plateau Lake-Watershed, Yunnan Research Academy of Eco-environmental Sciences, Kunming, China

Tóm tắt

Nhiều yếu tố stress liên quan đến biến đổi toàn cầu đang ảnh hưởng đến thành phần phân loại và sinh khối của phù du ở các hồ trên cao nguyên, chẳng hạn như mức độ bức xạ cực tím (UVR, 280–400 nm) cao hơn và nguy cơ thâm hụt dinh dưỡng. Mặc dù những ảnh hưởng hạn chế của UVR cao đối với phù du thường được công nhận, nhưng hiệu ứng này sẽ bị tác động bởi mức độ UVR biến đổi theo mùa và tình trạng dinh dưỡng. Trong nghiên cứu này, năng suất phù du tại chỗ được đo theo mùa dưới điều kiện bức xạ mặt trời đầy đủ và chỉ có ánh sáng nhìn thấy (bức xạ tích cực quang hợp, PAR) ở hồ Fuxian oligotrophic trên cao nguyên, vùng tây nam Trung Quốc. Để xác định các ảnh hưởng đơn lẻ của UVR và sự bổ sung dinh dưỡng cũng như ảnh hưởng kết hợp của chúng đến cộng đồng phù du, một thí nghiệm mesocosm (1 tháng) đã được thực hiện ngoài trời vào cuối mùa hè (mùa mưa). Thí nghiệm tương tác được thiết kế với hai điều trị bức xạ (UVR + PAR và PAR) và bốn điều trị dinh dưỡng (nước thô, bổ sung nitơ, bổ sung photpho, bổ sung cả nitơ và photpho). Kết quả của chúng tôi cho thấy rằng phản ứng của phù du đối với UVR phụ thuộc chủ yếu vào tính sẵn có của dinh dưỡng, điều này được thể hiện qua những phản ứng không đáng kể của năng suất phù du và tổng sinh khối đối với UVR trong nước thô oligotrophic từ hồ Fuxian, nhưng phản ứng tích cực của tổng sinh khối đối với UVR trong điều trị bổ sung dinh dưỡng. Hơn nữa, phản ứng của phù du đối với UVR cũng phụ thuộc vào loại taxa. Chlorophytes và dinoflagellates cho thấy phản ứng tích cực với UVR, trong khi chrysophytes lại khá nhạy cảm với UVR; diatoms không có phản ứng đáng kể đối với UVR trong tất cả các điều kiện dinh dưỡng, trong khi phản ứng của cyanobacteria đối với UVR phụ thuộc cao vào tình trạng dinh dưỡng. Nghiên cứu này nhấn mạnh rằng sự gia tăng nồng độ dinh dưỡng trong hồ oligotrophic không chỉ thúc đẩy trực tiếp sự phát triển của phù du mà còn kết hợp với UVR thấp trong mùa hè để có lợi cho sự phát triển của các taxa thâm hụt dinh dưỡng, dựa trên ảnh hưởng không tiêu cực của UVR đối với tổng sinh khối phù du và ảnh hưởng tích cực đối với các taxa thâm hụt dinh dưỡng.

Từ khóa

#phù du #bức xạ UV #dinh dưỡng #hồ Fuxian #thí nghiệm mesocosm

Tài liệu tham khảo

Anderson MJ, Willis TJ (2003) Canonical analysis of principal coordinates: a useful method of constrained ordination for ecology. Ecology 84:511–525 Archer SD, Stefels J, Airs RL, Lawson T, Smyth TJ, Rees AP, Geider RJ (2018) Limitation of dimethylsulfoniopropionate synthesis at high irradiance in natural phytoplankton communities of the Tropical Atlantic. Limnol Oceanogr 63:227–242 Ardiles V, Alcocer J, Vilaclara G, Oseguera LA, Velasco L (2012) Diatom fluxes in a tropical, oligotrophic lake dominated by large-sized phytoplankton. Hydrobiologia 679:77–90 Banaszak AT (2003) Photoprotective physiological and biochemical responses of aquatic organisms. In: Helbling EW, Zagarese HE (eds) UV effects in aquatic organisms and ecosystems. The Royal Society of Chemistry, Cambridge, pp 329–356 Barbieri ES, Villafañe VE, Helbling EW (2002) Experimental assessment of UV effects upon temperate marine phytoplankton when exposed to variable radiation regimes. Limnol Oceanogr 47:1648–1655 Bricker SB, Longstaff B, Dennison W, Jones A, Boicourt K, Wicks C, Woerner J (2008) Effects of nutrient enrichment in the nation's estuaries: A decade of change. Harmful Algae 8:21–32 Cabrerizo MJ, Carrillo P, Villafañe VE, Helbling EW (2014) Current and predicted global change impacts of UVR, temperature and nutrient inputs on photosynthesis and respiration of key marine phytoplankton groups. J Exp Mar Biol Ecol 461:371–380 Calbó J, Pagès D, González JA (2005) Empirical studies of cloud effects on UV radiation: a review. Rev Geophys 43:1–28 Carrillo P, Delgado-Molina JA, Medina-Sánchez JM, Bullejos FJ, Villar-Argaiz M (2008) Phosphorus inputs unmask negative effects of ultraviolet radiation on algae in a high mountain lake. Glob Chang Biol 14:423–439 Clarke KR (1993) Non-parametric multivariate analyses of changes in community structure. Aust J Ecol 18:117–143 Clerk S, Hall R, Quinlan R, Smol JP (2000) Quantitative inferences of past hypolimnetic anoxia and nutrient levels from a Canadan Precambrian shield lake. J Paleolimnol 23:319–336 D'Agostino PM, Woodhouse JN, Liew HT, Sehnal L, Pickford R, Wong HL, Burns BP, Neilan BA (2019) Bioinformatic, phylogenetic and chemical analysis of the UV-absorbing compounds scytonemin and mycosporine-like amino acids from the microbial mat communities of Shark Bay, Australia. Environ Microbiol 21:702–715 Delgado-Molina JA, Carrillo P, Medina-Sánchez JM, Villar-Argaiz M, Bullejos FJ (2009) Interactive effects of phosphorus loads and ambient ultraviolet radiation on the algal community in a high-mountain lake. J Plankton Res 31:619–634 Demmig-Adams B, Adams WW (2006) Photoprotection in an ecological context: the remarkable complexity of thermal energy dissipation. New Phytol 172:11–21 Dong J, Li GB, Song LR (2014) Historical changes of phytoplankton functional groups in Lake Fuxian, Lake Erhai and Lake Dianchi since 1960s. J Lake Sci 26:735–742 (in Chinese with English abstract) Doyle SA, Saros JE (2005) Interactive effects of temperature and nutrient limitation on the response of alpine phytoplankton growth to ultraviolet radiation. Limnol Oceanogr 50:1362–1367 Garcia-Gomez C, Gordillo FJ, Palma A, Lorenzo MR, Segovia M (2014) Elevated CO2 alleviates high PAR and UV stress in the unicellular chlorophyte Dunaliella tertiolecta. Photochem Photobiol Sci 13:1347–1358 Goss R, Jakob T (2010) Regulation and function of xantophyll cycle-dependent photoprotection in algae. Photosynth Res 106:103–122 Gregor J, Geriš R, Maršálek B, Heteša J, Marvan P (2005) In situ quantification of phytoplankton in reservoirs using a submersible spectrofluorometer. Hydrobiologia 548:141–151 Guan WC, Lu SH (2010) The short- and long-term response of Scrippsiella trochoidea (Pyrrophyta) to solar ultraviolet radiation. Photosynthetica 48:287–293 Häder DP, Kumar HD, Smith RC, Worrest RC (2007) Effects of solar UV radiation on aquatic ecosystems and interactions with climate change. Photochem Photobiol Sci 6:267–285 Häder DP, Helbling EW, Williamson CE, Worrest RC (2011) Effects of UV radiation on aquatic ecosystems and interactions with climate change. Photochem Photobiol Sci 10:242–260 Harrison JW, Smith REH (2009) Effects of ultraviolet radiation on the productivity and composition of freshwater phytoplankton communities. Photochem Photobiol Sci 8:12–18 Harrison JW, Smith RE (2013) Effects of nutrients and irradiance on PSII variable fluorescence of lake phytoplankton assemblages. Aquat Sci 75:399–411 Helbling EW, Barbieri ES, Marcoval MA, Gonçalves RJ, Villafañe VE (2005) Impact of solar ultraviolet radiation on marine phytoplankton of Patagonia, Argentina. Photochem Photobiol 81:807–818 Helbling EW, Farías ME, Zenoff MVF, Villafañe VE (2006) In situ responses of phytoplankton from the subtropical Lake La Angostura (Tucumán, Argentina) in relation to solar ultraviolet radiation exposure and mixing conditions. Hydrobiologia 559:123–134 Hu H, Wei Y (2006) The freshwater algae of China: systematics, taxonomy and ecology. Science press, Beijing Järvinen M, Drakare S, Free G, Lyche-Solheim A, Phillips G, Skjelbred B, Mischke U, Ott I, Poikane S, Søndergaard M, Pasztaleniec A, Wichelen JV, Portielje R (2013) Phytoplankton indicator taxa for reference conditions in Northern and Central European lowland lakes. Hydrobiologia 704:97–113 Jones RI (2000) Mixotrophy in planktonic protists: an overview. Freshw Biol 45:219–226 Kennish MJ, Brush MJ, Moore KA (2014) Drivers of change in shallow coastal photic systems: an introduction to a special issue. Estuar Coasts 37:3–19 Kobayashi T, Ralph TJ, Ryder DS, Hunter SJ (2013) Gross primary productivity of phytoplankton and planktonic respiration in inland floodplain wetlands of southeast Australia: habitat-dependent patterns and regulating processes. Ecol Res 28:833–843 Korbee N, Mata MT, Figueroa FL (2010) Photoprotection mechanisms against ultraviolet radiation in Heterocapsa sp. (Dinophyceae) are influenced by nitrogen availability: Mycosporine-like amino acids vs. xanthophyll cycle. Limnol Oceanogr 55:899–908 Korbee N, Carrillo P, Mata MT, Rosillo S, Medina-Sánchezc JM, Figueroa FL (2012) Effects of ultraviolet radiation and nutrients on the structure–function of phytoplankton in a high mountain lake. Photochem Photobiol Sci 11:1087–1098 Krammer K, Lange-Bertalot H (1991) Bacillariophyceae. 2/3. Centrales, Fragilariaceae, Eunotiaceae. Gustav Fischer Verlag, Jena, 576 pp Krawczyk WE, Ford DC (2007) Correlating specific conductivity with total hardness in gypsum karst waters. Earth Surf Process Landf 32:612–620 Lee WM, An YJ (2013) Effects of zinc oxide and titanium dioxide nanoparticles on green algae under visible, UVA, and UVB irradiations: no evidence of enhanced algal toxicity under UV pre-irradiation. Chemosphere 91:536–544 Li G, Wu Y, Gao K (2009) Effects of Typhoon Kaemi on coastal phytoplankton assemblages in the South China Sea, with special reference to the effects of solar UV radiation. J Geophys Res Biogeosci 114:G04029 Li W, Yang Y, Li ZZ, Xu JT, Gao KS (2016) Effects of seawater acidification on the growth rates of the diatom Thalassiosira (conticribra) weissflogii, under different nutrient, light, and UV radiation regimes. J Appl Phycol 29:1–10 Litchman E, Neale PJ, Banaszak AT (2002) Increased sensitivity to ultraviolet radiation in nitrogen-limited dinoflagellates: photoprotection and repair. Limnol Oceanogr 47:86–94 Liu G, Hu Z (2012) Flora algarum sinicarum aquae dulcis. Tomus 15. Chlorophyta: Chlorooccales (II): Tetrasporales, Dichotomosiphonales, Cladophorales. Science Press, Beijing Liu T, Li YC, Liu QS, Wang B, Huang RQ (2001) Investigation on solar ultraviolet radiation of Yunnan, China. J Yunnan Norm Univ 21:37–42 (in Chinese with English abstract) Luo W, Bock C, Li HR, Padisák J, Krienitz L (2011) Molecular and microscopic diversity of planktonic eukaryotes in the oligotrophic Lake Stechlin (Germany). Hydrobiologia 661:133–143 Ma JR, Qin BQ, Paerl HW, Brookes JD, Wu P, Zhou J, Deng JM, Guo JS, Li Z (2015) Green algal over cyanobacterial dominance promoted with nitrogen and phosphorus additions in a mesocosm study at lake Taihu, China. Environ Sci Pollut Res 22:5041–5049 Maileht K, NõGes T, NõGes P, Ott I, Mischke U, Carvalho L, Dudley B (2013) Water colour, phosphorus and alkalinity are the major determinants of the dominant phytoplankton species in European lakes. Hydrobiologia 704:115–126 Marcoval MA, Villafañe VE, Helbling EW (2007) Interactive effects of ultraviolet radiation and nutrient addition on growth and photosynthesis performance of four species of marine phytoplankton. J Photochem Photobiol B 89:78–87 Marcoval MA, VillafañE VE, Helbling EW (2008) Combined effects of solar ultraviolet radiation and nutrients addition on growth, biomass and taxonomic composition of coastal marine phytoplankton communities of Patagonia. J Photochem Photobiol B 91:157–166 McKenzie R, Aucamp PJ, Bais A, Björn LO, Ilyas M, Madronich S (2011) Ozone depletion and climate change: impacts on UV radiation. Photochem Photobiol Sci 10:182–198 Niu Y, Shen H, Chen J, Xie P, Yang X, Tao M, Ma ZM, Qi M (2011) Phytoplankton community succession shaping bacterioplankton community composition in Lake Taihu, China. Water Res 45:4169–4182 Nürnberg GK (1996) Trophic state of clear and colored, soft-and hardwater lakes with special consideration of nutrients, anoxia, phytoplankton and fish. Lake Reserv Manag 12:432–447 R Development Core Team (2019) R: a language and environment for statistical computing. R Foundation for Statistical Computing. Available online: http://www.r-project.org/ Richards TA, Vepritskiy AA, Gouliamova DE, Nierzwicki-Bauer SA (2005) The molecular diversity of freshwater picoeukaryotes from an oligotrophic lake reveals diverse, distinctive and globally dispersed lineages. Environ Microbiol 7:1413–1425 Rose KC, Williamson CE, Saros JE, Sommaruga R, Fischer JM (2009) Differences in UV transparency and thermal structure between alpine and subalpine lakes: implications for organisms. Photochem Photobiol Sci 8:1244–1256 Sandmann G (2019) Antioxidant protection from UV-and light-stress related to carotenoid structures. Antioxidants 8:219 Segovia M, Teresa M, Armando P, García-Gómez C, Lorenzo R, Rivera A, Figueroa FL (2015) Dunaliella tertiolecta (Chlorophyta) avoids cell death under ultraviolet radiation (UVR) by triggering alternative photoprotective mechanisms. Photochem Photobiol 91:1389–1402 Shi Z (2013) Flora algarum sinicarum aquae dulcis, tomus 16, Bacillariophyta: Cymbellaceae. Science Press, Beijing Shi Z, Wang Q, Xie S, Dai J (1999) Flora Algarum Sinicarum Aquae Dulcis, Tomus 6, Euglenophyta. Science Press, Beijing Shi XL, Li SN, Li HB, Chen FZ, Wu QL (2019) The community structure of picophytoplankton in Lake Fuxian, a deep and oligotrophic mountain lake. Front Microbiol 10:2016. https://doi.org/10.3389/fmicb.2019.02016 Singh SP, Häder DP, Sinha RP (2010) Cyanobacteria and ultraviolet radiation (UVR) stress: mitigation strategies. Ageing Res Rev 9:79–90 Sinha RP, Häder DP (2008) UV-protectants in cyanobacteria. Plant Sci 174:278–289 Sobrino C, Neale PJ (2007) Short-term and long-term effects of temperature on photosynthesis in the diatom Thalassiosira pseudonana under UVR exposures. J Phycol 43:426–436 State Environmental Protection Administration of China (2002) Water and wastewater monitoring and analysis methods, 4th edn. China Environmental Science Press, Beijing, p 783 (in Chinese) Stebbing ARD (1982) Hormesis — the stimulation of growth by low levels of inhibitors. Sci Total Environ 22:213–234 Torremorell A, Llames ME, PÉRez GL, Escaray R, Bustingorry J, Zagarese H (2009) Annual patterns of phytoplankton density and primary production in a large, shallow lake: the central role of light. Freshw Biol 54:437–449 Villafañe VE, Andrade M, Lairana V, Zaratti F, Helbling EW (1999) Inhibition of phytoplankton photosynthesis by solar ultraviolet radiation: studies in Lake Titicaca, Bolivia. Freshw Biol 42:215–224 Wang WL, Yang X, Huang LC, Qin J, Zhou QC (2020) Attenuation of ultraviolet radiation and photosynthetically active radiation in six Yunnan Plateau lakes of China based on seasonal field investigations. J Limnol 79:151-163 https://doi.org/10.4081/jlimnol.2020.1951. Wasmund N, Nausch G, Voss M (2012) Upwelling events may cause cyanobacteria blooms in the Baltic Sea. J Mar Syst 90:67–76 Wei Y (2017) Flora algarum sinicarum aquae dulcis. Tomus 17. Chlorophyta: Desmidiales, Desmidiaceae (II). Science Press, Beijing Wei Y (2018) Flora algarum sinicarum aquae dulcis. Tomus 21. Chrysophyta (II). Science Press, Beijing Weyhenmeyer GA, Willén E, Sonesten I (2004) Effects of an extreme precipitation event on water chemistry and phytoplankton in the Swedish Lake Mälaren. Boreal Environ Res 9:409–420 Williamson CE, Overholt EP, Pilla RM, Leach TH, Brentrup JA, Knoll LB, Mette EM, Moeller RE (2016) Ecological consequences of long-term browning in lakes. Sci Rep-UK 5:18666 Wolfe AP, Siver PA (2013) A hypothesis linking chrysophyte microfossils to lake carbon dynamics on ecological and evolutionary time scales. Glob Planet Chang 111:189–198 Xu J, Gao K (2010) Use of UV-A energy for photosynthesis in the red macroalga Gracilaria lemaneiformis. Photochem Photobiol 86:580–585 Xu H, Paerl HW, Qin B, Zhu G, Gao G (2010) Nitrogen and phosphorus inputs control phytoplankton growth in eutrophic lake Taihu, China. Limnol Oceanogr 55:420–432 Zhang ZS, Huang XF (1995) Research methods of freshwater plankton. Science Press, Beijing, pp 342–344 (in Chinese) Zhang YL, Yin Y, Zhang EL, Zhu GW, Liu ML, Feng LQ, Qin BQ, Liu XH (2011) Spectral attenuation of ultraviolet and visible radiation in lakes in the Yunnan Plateau, and the middle and lower reaches of the Yangtze River, China. Photochem Photobiol Sci 10:469–482 Zhang Y, Peng CR, Wang J, Huang S, Hu Y, Zhang JL, Li DH (2019) Temperature and silicate are significant driving factors for the seasonal shift of dominant diatoms in a drinking water reservoir. J Oceanol Limnol 37:568–579 Zhang YL, Shi K, Zhou QC, Zhou YQ, Zhang YB, Qin BQ, Deng JM (2020) Decreasing underwater ultraviolet radiation exposure strongly driven by increasing ultraviolet attenuation in lakes in eastern and southwest China. Sci Total Environ 720:137694. https://doi.org/10.1016/j.scitotenv.2020.137694. Zhou QC, Zhang YL, Li KD, Huang LC, Yang FL, Zhou YY, Chang JJ (2018) Seasonal and spatial distributions of euphotic zone and long-term variations in water transparency in a clear oligotrophic lake Fuxian, China. J Environ Sci-China 72:185–197 Zhou QC, Wang WL, Huang LC, Zhang YL, Qin J, Li KD, Chen L (2019) Spatial and temporal variability in water transparency in Yunnan Plateau lakes, China. Aquat Sci 81:36 Zhu H (2007) Flora algarum sinicarum aquae dulcis. Tomus 9. Cyanophyta: Hormogonophyceae. Science Press, Beijing Zhu Z, Fu FX, Qu PP, Mak EWK, Jiang HB, Zhang RF, Zhu ZY, Gao KS, Hutchins DA (2020) Interactions between ultraviolet radiation exposure and phosphorus limitation in the marine nitrogen-fixing cyanobacteria Trichodesmium and Crocosphaera. Limnol Oceanogr 65:363–376 https://doi.org/10.1002/lno.11304